Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LCH18_RS13605 Genome accession   NZ_CP083947
Coordinates   2798108..2799229 (+) Length   373 a.a.
NCBI ID   WP_224467307.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain CSUSB1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2793108..2804229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH18_RS13590 (LCH18_13590) - 2795308..2796042 (-) 735 WP_224467302.1 hypothetical protein -
  LCH18_RS13595 (LCH18_13595) - 2796232..2796921 (-) 690 WP_224467303.1 YggS family pyridoxal phosphate-dependent enzyme -
  LCH18_RS13600 (LCH18_13600) pilT 2797044..2798081 (+) 1038 WP_224467305.1 type IV pilus twitching motility protein PilT Machinery gene
  LCH18_RS13605 (LCH18_13605) pilU 2798108..2799229 (+) 1122 WP_224467307.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LCH18_RS13610 (LCH18_13610) fur 2799289..2799726 (-) 438 WP_004694866.1 ferric iron uptake transcriptional regulator -
  LCH18_RS13615 (LCH18_13615) - 2799839..2800237 (+) 399 WP_087528300.1 outer membrane protein assembly factor BamE -
  LCH18_RS13620 (LCH18_13620) - 2800284..2800607 (-) 324 WP_224467308.1 RnfH family protein -
  LCH18_RS13625 (LCH18_13625) - 2800607..2801689 (-) 1083 WP_224467310.1 hypothetical protein -
  LCH18_RS13630 (LCH18_13630) - 2801832..2802287 (-) 456 WP_078389448.1 bacteriohemerythrin -
  LCH18_RS13635 (LCH18_13635) - 2802562..2803410 (-) 849 WP_224467311.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41876.14 Da        Isoelectric Point: 6.6664

>NTDB_id=608765 LCH18_RS13605 WP_224467307.1 2798108..2799229(+) (pilU) [Acinetobacter johnsonii strain CSUSB1]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGDRLLHASANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=608765 LCH18_RS13605 WP_224467307.1 2798108..2799229(+) (pilU) [Acinetobacter johnsonii strain CSUSB1]
ATGGACTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCTTC
CATGAAAATTAACGGTCAAATTGTGCCCGTGGCTAAGGGTAAGCTGACCGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGAAAAACAGCGTAAAGAATTTGCAGATACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCATTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGACGAGTTAAAACTCCCGCCTGTACTCAAAGAATTAGCCATGACCAAACGTGGCATCATCATCTTTGTGGGTGCAACAG
GTACGGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGCCATATCATTACCATTGAA
GACCCAATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACTGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAAGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCGATTGCCTTTGCTGAAACAGGTCACTTGGTATTTGCAACGCTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTTTTTGAAGCAGACCGTCATAACCAATTGTTTATGGACTTGTCTTTAAACCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCTGATGGTAATTCACGTCGCGCTGCAATTGAGATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCATGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTATTCGATTTGTACAAAGCTAAGCAGATTAGCTATAAAGATGCGCTGAAACACGCCGATTCCCCGAATGACTTACG
TTTACAAATCAAGTTGTCCGAAGAAGGCGGTGATCGTCTATTGCATGCCAGTGCAAACATCACGTTTGACGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.984

100

0.78
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.464

0.678
  pilU Vibrio cholerae strain A1552

55.405

99.196

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.124

90.617

0.373
  pilT Legionella pneumophila strain ERS1305867

41.124

90.617

0.373
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365