Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8O61_RS06255 Genome accession   NZ_CP083804
Coordinates   1459597..1460634 (+) Length   345 a.a.
NCBI ID   WP_003471666.1    Uniprot ID   A0A0K2ZRY0
Organism   Xanthomonas cerealis pv. cerealis strain ICMP 16317     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1454597..1465634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O61_RS06230 (K8O61_06255) soxR 1455281..1455718 (+) 438 WP_058195935.1 redox-sensitive transcriptional activator SoxR -
  K8O61_RS06235 (K8O61_06260) - 1455851..1456306 (-) 456 WP_237655807.1 DUF4426 domain-containing protein -
  K8O61_RS06240 (K8O61_06265) proC 1456321..1457163 (-) 843 WP_039005509.1 pyrroline-5-carboxylate reductase -
  K8O61_RS06245 (K8O61_06270) - 1457293..1458345 (-) 1053 WP_230812424.1 hypothetical protein -
  K8O61_RS06250 (K8O61_06275) - 1458820..1459470 (-) 651 WP_237656051.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O61_RS06255 (K8O61_06280) pilT 1459597..1460634 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O61_RS06260 (K8O61_06285) pilU 1460795..1461925 (+) 1131 WP_039005505.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8O61_RS06265 (K8O61_06290) - 1462352..1462891 (-) 540 WP_039005504.1 DNA-3-methyladenine glycosylase I -
  K8O61_RS06270 (K8O61_06295) - 1462907..1464712 (-) 1806 WP_058195940.1 DUF4153 domain-containing protein -
  K8O61_RS06275 (K8O61_06300) - 1464820..1465386 (+) 567 WP_039005503.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38599.24 Da        Isoelectric Point: 6.6650

>NTDB_id=607993 K8O61_RS06255 WP_003471666.1 1459597..1460634(+) (pilT) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQARDYAKDKRVFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=607993 K8O61_RS06255 WP_003471666.1 1459597..1460634(+) (pilT) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGGATATCGCTGAACTATTGGCGTTCTCGGTCAAGAACAAAGCGTCCGACCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATCCGGGTCGATGGCGACGTGCGCCGCATCAATATCCCGGCACTGGACCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAAGAGTTCCTCGAAGTGGACTTCTCGTTCGAGATTCCCAGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATCCCGTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTTCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGACCCGGATATCATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACTATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCGTGGGAGATCATGGTCGGCACCCCGGCGATCCGCAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCCAGCAGTACGGCATGCAGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGGGTCTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K2ZRY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

78.779

99.71

0.786
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397