Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8O61_RS06260 Genome accession   NZ_CP083804
Coordinates   1460795..1461925 (+) Length   376 a.a.
NCBI ID   WP_039005505.1    Uniprot ID   A0A514E9W0
Organism   Xanthomonas cerealis pv. cerealis strain ICMP 16317     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1455795..1466925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O61_RS06235 (K8O61_06260) - 1455851..1456306 (-) 456 WP_237655807.1 DUF4426 domain-containing protein -
  K8O61_RS06240 (K8O61_06265) proC 1456321..1457163 (-) 843 WP_039005509.1 pyrroline-5-carboxylate reductase -
  K8O61_RS06245 (K8O61_06270) - 1457293..1458345 (-) 1053 WP_230812424.1 hypothetical protein -
  K8O61_RS06250 (K8O61_06275) - 1458820..1459470 (-) 651 WP_237656051.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O61_RS06255 (K8O61_06280) pilT 1459597..1460634 (+) 1038 WP_003471666.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O61_RS06260 (K8O61_06285) pilU 1460795..1461925 (+) 1131 WP_039005505.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8O61_RS06265 (K8O61_06290) - 1462352..1462891 (-) 540 WP_039005504.1 DNA-3-methyladenine glycosylase I -
  K8O61_RS06270 (K8O61_06295) - 1462907..1464712 (-) 1806 WP_058195940.1 DUF4153 domain-containing protein -
  K8O61_RS06275 (K8O61_06300) - 1464820..1465386 (+) 567 WP_039005503.1 YqgE/AlgH family protein -
  K8O61_RS06280 (K8O61_06305) ruvX 1465379..1465876 (+) 498 WP_237655808.1 Holliday junction resolvase RuvX -
  K8O61_RS06285 (K8O61_06310) - 1465896..1466843 (+) 948 WP_237655809.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41943.28 Da        Isoelectric Point: 6.8811

>NTDB_id=607994 K8O61_RS06260 WP_039005505.1 1460795..1461925(+) (pilU) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
MSTIDFTSFLKLMAHQKASDLFITAGMPPAIKVHGKITPITQTPLTAPQSRDLVLNVMTPAQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRVINFFPEDRRSQLLMDLSLNMRGVVAQQLIPTPDGKSRRVAMEIMLGTPLIQDYIREGEIHKLKDVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRHADSQNEVRLRIKLAQGGDARTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=607994 K8O61_RS06260 WP_039005505.1 1460795..1461925(+) (pilU) [Xanthomonas cerealis pv. cerealis strain ICMP 16317]
ATGAGCACCATCGACTTCACCTCGTTCCTGAAACTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCGCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCACCCCGATCACGCAGACGCCGCTGACCGCGCCGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACCCATGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAGACGCGCATCCC
CACCGTCGACGAACTGAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATTATCATCTTCGTCGGTG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGATACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCTCCTTCGCCGAGACTGGCCATCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGGGTCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTGTCGTTGAACATGCGCGGCGTGGT
CGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGAGCCGGCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGATAC
AGGATTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGACGTGATGAAGGAATCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCGCCACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCGCCCAGGGCGGCGACGCCAGGACCCTGGCGCAGGGCATGGACGGGGTGGAGATCGCGG
AGGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A514E9W0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.312

98.138

0.641
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42

93.085

0.391
  pilT Acinetobacter baumannii strain A118

40.698

91.489

0.372
  pilT Acinetobacter nosocomialis M2

40.698

91.489

0.372
  pilT Acinetobacter baumannii D1279779

40.698

91.489

0.372
  pilT Pseudomonas aeruginosa PAK

41.298

90.16

0.372