Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAG74_RS18610 Genome accession   NZ_CP083461
Coordinates   4071358..4072392 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain SW3814     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4066358..4077392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAG74_RS18580 (LAG74_18610) glnK 4066939..4067277 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  LAG74_RS18585 (LAG74_18615) arfB 4067479..4067895 (+) 417 WP_224968835.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LAG74_RS18590 (LAG74_18620) - 4067928..4068419 (+) 492 WP_224968837.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LAG74_RS18595 (LAG74_18625) - 4068779..4069204 (-) 426 WP_005340360.1 hypothetical protein -
  LAG74_RS18600 (LAG74_18630) yaaA 4069399..4070172 (-) 774 WP_224968839.1 peroxide stress protein YaaA -
  LAG74_RS18605 (LAG74_18635) pilU 4070220..4071329 (-) 1110 WP_224970231.1 type IVa pilus ATPase TapU Machinery gene
  LAG74_RS18610 (LAG74_18640) pilT 4071358..4072392 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  LAG74_RS18615 (LAG74_18645) - 4072432..4073133 (+) 702 WP_224968841.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAG74_RS18620 (LAG74_18650) proC 4073275..4074099 (+) 825 WP_224968843.1 pyrroline-5-carboxylate reductase -
  LAG74_RS18625 (LAG74_18655) - 4074111..4074671 (+) 561 WP_224968845.1 YggT family protein -
  LAG74_RS18630 (LAG74_18660) yggU 4074671..4074973 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  LAG74_RS18635 (LAG74_18665) - 4075017..4075436 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  LAG74_RS18640 (LAG74_18670) - 4075538..4076734 (-) 1197 WP_160881766.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=606024 LAG74_RS18610 WP_005340354.1 4071358..4072392(-) (pilT) [Aeromonas veronii strain SW3814]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=606024 LAG74_RS18610 WP_005340354.1 4071358..4072392(-) (pilT) [Aeromonas veronii strain SW3814]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATTGCCGAGTTCCCGCGTGGTCTGGTGTTGGTGACCGGCCCGACCGGCT
CGGGTAAGTCGACCACTTTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAGGAC
CCTATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGGGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTTTGG
CCATGACCGCCGCCGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAGGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCAGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATTC
GGGAGGACAAGATTGCCCAGCTTTACTCGGTGATCCAAACCGGGATGACCCACGGTATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTGGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407