Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LAG74_RS18605 Genome accession   NZ_CP083461
Coordinates   4070220..4071329 (-) Length   369 a.a.
NCBI ID   WP_224970231.1    Uniprot ID   -
Organism   Aeromonas veronii strain SW3814     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4065220..4076329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAG74_RS18575 (LAG74_18605) - 4065673..4066680 (-) 1008 WP_005357960.1 Fe(3+) ABC transporter substrate-binding protein -
  LAG74_RS18580 (LAG74_18610) glnK 4066939..4067277 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  LAG74_RS18585 (LAG74_18615) arfB 4067479..4067895 (+) 417 WP_224968835.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  LAG74_RS18590 (LAG74_18620) - 4067928..4068419 (+) 492 WP_224968837.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  LAG74_RS18595 (LAG74_18625) - 4068779..4069204 (-) 426 WP_005340360.1 hypothetical protein -
  LAG74_RS18600 (LAG74_18630) yaaA 4069399..4070172 (-) 774 WP_224968839.1 peroxide stress protein YaaA -
  LAG74_RS18605 (LAG74_18635) pilU 4070220..4071329 (-) 1110 WP_224970231.1 type IVa pilus ATPase TapU Machinery gene
  LAG74_RS18610 (LAG74_18640) pilT 4071358..4072392 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  LAG74_RS18615 (LAG74_18645) - 4072432..4073133 (+) 702 WP_224968841.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAG74_RS18620 (LAG74_18650) proC 4073275..4074099 (+) 825 WP_224968843.1 pyrroline-5-carboxylate reductase -
  LAG74_RS18625 (LAG74_18655) - 4074111..4074671 (+) 561 WP_224968845.1 YggT family protein -
  LAG74_RS18630 (LAG74_18660) yggU 4074671..4074973 (+) 303 WP_021230942.1 DUF167 family protein YggU -
  LAG74_RS18635 (LAG74_18665) - 4075017..4075436 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41060.12 Da        Isoelectric Point: 6.5043

>NTDB_id=606023 LAG74_RS18605 WP_224970231.1 4070220..4071329(-) (pilU) [Aeromonas veronii strain SW3814]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANFAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=606023 LAG74_RS18605 WP_224970231.1 4070220..4071329(-) (pilU) [Aeromonas veronii strain SW3814]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGTGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGTTATATCCGCACCAAGGAGGCCAACTTCGCGATTTATCGCGAGGCACTTGGCCGT
TTTCGGGTCAGTGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCTACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACCCAAGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAC
CCGGTGGAGTTCGTTCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGTGAGATCCGCAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAGCACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATTGTCGCCCAGCA
GCTGGTGCCAAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACATTTGATCAGGCG
CTTTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCCCTCGCCCACGCCGACTCGGCCAACGACCTGCGATT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGCACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

60.684

95.122

0.577
  pilU Acinetobacter baylyi ADP1

57.507

95.664

0.55
  pilT Legionella pneumophila strain Lp02

41.108

92.954

0.382
  pilT Legionella pneumophila strain ERS1305867

41.108

92.954

0.382
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.118

91.87

0.369
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363