Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAD35_RS02450 Genome accession   NZ_CP083451
Coordinates   537675..538718 (-) Length   347 a.a.
NCBI ID   WP_224151151.1    Uniprot ID   -
Organism   Comamonas odontotermitis strain WLL     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 532675..543718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAD35_RS02425 (LAD35_02425) - 533163..533762 (+) 600 WP_224151147.1 SIS domain-containing protein -
  LAD35_RS02430 (LAD35_02430) - 533762..534580 (+) 819 WP_396022785.1 BON domain-containing protein -
  LAD35_RS02435 (LAD35_02435) - 534669..535688 (-) 1020 WP_224151148.1 NAD(P)-dependent oxidoreductase -
  LAD35_RS02440 (LAD35_02440) pilU 535692..536828 (-) 1137 WP_224151149.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LAD35_RS02445 (LAD35_02445) - 536913..537521 (-) 609 WP_224151150.1 cyclic nucleotide-binding domain-containing protein -
  LAD35_RS02450 (LAD35_02450) pilT 537675..538718 (-) 1044 WP_224151151.1 type IV pilus twitching motility protein PilT Machinery gene
  LAD35_RS02455 (LAD35_02455) - 538757..539485 (+) 729 WP_224151152.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAD35_RS02460 (LAD35_02460) - 539639..540775 (+) 1137 WP_224151153.1 aminotransferase class V-fold PLP-dependent enzyme -
  LAD35_RS02465 (LAD35_02465) - 540857..541273 (+) 417 WP_224151154.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  LAD35_RS02470 (LAD35_02470) - 541379..542179 (-) 801 WP_224151155.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38277.95 Da        Isoelectric Point: 6.7744

>NTDB_id=605954 LAD35_RS02450 WP_224151151.1 537675..538718(-) (pilT) [Comamonas odontotermitis strain WLL]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKQVHAMVYDIMSDAQRKQYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAGAVFRTIPSKILTLEQLNAPKIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNYLNENEYGHILTVED
PIEFVHESKKCLINQREVGPQTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSNAVGMQTLDQN
LTDLVRRNVISPAEARGKAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=605954 LAD35_RS02450 WP_224151151.1 537675..538718(-) (pilT) [Comamonas odontotermitis strain WLL]
GTGGATATCACGCAATTGCTGGCATTCAGCGTCAAAAACAAGGCTTCTGACTTGCACTTGTCGGCCGGCCTGCCGCCAAT
GATCCGGGTTCATGGCGATGTACGCCGCATCAACGTCGATCCGCTGGACCACAAGCAGGTGCACGCGATGGTCTACGACA
TCATGAGCGATGCCCAGCGAAAGCAGTATGAAGAGTTCCTGGAAGTCGACTTTTCGTTCGAGATCGACGGTCTGGCGCGC
TTTCGCGTCAACGCTTTCAACCAGAACCGCGGCGCAGGCGCGGTGTTTCGTACCATTCCGAGCAAGATTCTCACGCTGGA
ACAGCTGAACGCACCGAAGATCTTTGCCGAGTTGTCGCTCAAGCCGCGCGGTCTGGTGCTGGTGACGGGCCCGACTGGTT
CGGGCAAATCCACCACCCTGGCAGCCATGGTGAACTACCTGAACGAAAACGAATACGGCCACATCCTCACGGTGGAGGAC
CCCATCGAATTCGTGCACGAGTCCAAGAAATGCCTCATCAACCAGCGCGAAGTGGGACCGCAGACCCTGTCTTTCAGTGC
CGCGCTGCGTTCCGCGCTGCGTGAAGACCCGGACGCGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATTCGCCTGG
CCATGACGGCGGCAGAAACCGGTCACTTGGTTTTCGGCACCTTGCACACCTCGTCGGCCGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCTGCCGAAGAAAAGGAAATGGTGCGTGCGATGCTGTCCGAATCACTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAAACCAAGGACGGCCAGGGCCGCGTGGCTGCGCACGAAATCATGCTGGGCACCCCCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTGGCGCAGATGTATTCCACCATCCAGACCAGCAATGCCGTGGGTATGCAGACCCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAACGTGATCAGCCCGGCAGAGGCACGCGGCAAGGCCAAGACACCGGAAAACTTCCCGGG
TTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.391

99.424

0.769
  pilT Acinetobacter baumannii D1279779

77.391

99.424

0.769
  pilT Acinetobacter baumannii strain A118

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.536

99.424

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.639

97.406

0.669
  pilT Vibrio cholerae strain A1552

68.639

97.406

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

43.478

99.424

0.432
  pilU Acinetobacter baylyi ADP1

41.618

99.712

0.415
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.542

0.409