Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LAD35_RS02440 Genome accession   NZ_CP083451
Coordinates   535692..536828 (-) Length   378 a.a.
NCBI ID   WP_224151149.1    Uniprot ID   -
Organism   Comamonas odontotermitis strain WLL     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530692..541828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAD35_RS02415 (LAD35_02415) rsmI 531561..532508 (-) 948 WP_224151145.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  LAD35_RS02420 (LAD35_02420) - 532535..532957 (+) 423 WP_224151146.1 YraN family protein -
  LAD35_RS02425 (LAD35_02425) - 533163..533762 (+) 600 WP_224151147.1 SIS domain-containing protein -
  LAD35_RS02430 (LAD35_02430) - 533762..534580 (+) 819 WP_396022785.1 BON domain-containing protein -
  LAD35_RS02435 (LAD35_02435) - 534669..535688 (-) 1020 WP_224151148.1 NAD(P)-dependent oxidoreductase -
  LAD35_RS02440 (LAD35_02440) pilU 535692..536828 (-) 1137 WP_224151149.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LAD35_RS02445 (LAD35_02445) - 536913..537521 (-) 609 WP_224151150.1 cyclic nucleotide-binding domain-containing protein -
  LAD35_RS02450 (LAD35_02450) pilT 537675..538718 (-) 1044 WP_224151151.1 type IV pilus twitching motility protein PilT Machinery gene
  LAD35_RS02455 (LAD35_02455) - 538757..539485 (+) 729 WP_224151152.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAD35_RS02460 (LAD35_02460) - 539639..540775 (+) 1137 WP_224151153.1 aminotransferase class V-fold PLP-dependent enzyme -
  LAD35_RS02465 (LAD35_02465) - 540857..541273 (+) 417 WP_224151154.1 FKBP-type peptidyl-prolyl cis-trans isomerase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41893.29 Da        Isoelectric Point: 6.6981

>NTDB_id=605953 LAD35_RS02440 WP_224151149.1 535692..536828(-) (pilU) [Comamonas odontotermitis strain WLL]
MERDQASKFINDLLKLMVQRNGSDLFITADFPPAIKIDGKVTKVSSQPLSPNHTLTLARSIMSDRQVAEFEKTKECNFAI
SPAGIGRFRVNAFVQQGSVGLVLRTIPLTLPTIDGLGVPQVLKDVAMAKRGLCIMVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRGMVSQRLLPKQDSKGRVAAVEVMLNTPLIADLIFKGEVGEIKEIMKKSRNLG
MQTFDQSLFDLYEANMITYEDALRNADSVNDLRLQIKLGSQRAKRTDLAEGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=605953 LAD35_RS02440 WP_224151149.1 535692..536828(-) (pilU) [Comamonas odontotermitis strain WLL]
ATGGAACGGGACCAGGCCAGTAAATTCATCAACGATTTGCTCAAGCTGATGGTGCAGCGCAATGGCAGTGACTTGTTTAT
CACTGCCGATTTTCCGCCCGCCATCAAGATCGACGGCAAGGTCACCAAGGTATCCAGCCAGCCGCTCTCGCCCAACCATA
CGCTGACGCTCGCGCGCTCGATCATGAGCGACCGCCAGGTTGCCGAGTTCGAAAAGACCAAGGAATGCAATTTCGCGATC
TCGCCCGCAGGCATCGGGCGATTCCGAGTCAACGCCTTCGTGCAGCAGGGCAGTGTAGGCCTGGTGCTGCGTACCATTCC
GCTGACCCTGCCAACCATCGATGGCCTGGGTGTGCCCCAGGTGCTCAAGGACGTGGCCATGGCCAAGCGCGGGCTGTGCA
TCATGGTGGGCGCCACGGGTTCAGGCAAATCCACCACCCTGGCCGCCATGGTGGACTGGCGCAACGAAAACTCCTTCGGC
CACATCATCACGGTCGAAGACCCGGTGGAATTCGTCCACCCCCACAAGAATTGCGTCGTGACCCAGCGCGAAGTGGGGCT
GGACACCGAAAGCTGGGAAGCCGCACTGAAGAACACGCTGCGTCAGGCGCCCGATGTGATCCTGATGGGTGAAATCCGTG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCGACTCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCATCAACTTCTTCCCTGAAGAGCGCCGCGCGCAGCTGCTGATGGACCTTTCGCTGAATCT
GCGCGGCATGGTGTCGCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGCCGCGTGGCCGCCGTGGAAGTGATGCTGAATA
CGCCGCTGATTGCGGATCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACTTGGGC
ATGCAGACCTTTGACCAGTCGCTGTTCGACCTGTATGAAGCCAACATGATCACCTACGAAGACGCGCTGCGCAATGCCGA
CTCGGTCAATGACCTGCGCCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGCGCACCGACCTGGCCGAAGGCACCGAGC
ATCTCACAATCGTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(51-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.115

96.825

0.611
  pilU Acinetobacter baylyi ADP1

56.546

94.974

0.537
  pilU Vibrio cholerae strain A1552

54.885

92.063

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Acinetobacter nosocomialis M2

43.243

88.095

0.381
  pilT Acinetobacter baumannii D1279779

43.243

88.095

0.381
  pilT Acinetobacter baumannii strain A118

43.243

88.095

0.381
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.543

89.153

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.711

84.127

0.368
  pilT Vibrio cholerae strain A1552

43.711

84.127

0.368