Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LAJ50_RS05605 Genome accession   NZ_CP083376
Coordinates   1265816..1266853 (+) Length   345 a.a.
NCBI ID   WP_130517185.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. X-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1260816..1271853
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ50_RS05580 (LAJ50_05585) - 1261598..1262968 (-) 1371 WP_224096489.1 MFS transporter -
  LAJ50_RS05585 (LAJ50_05590) soxR 1263062..1263496 (+) 435 WP_138654985.1 redox-sensitive transcriptional activator SoxR -
  LAJ50_RS05590 (LAJ50_05595) - 1263516..1263986 (-) 471 WP_138654987.1 DUF4426 domain-containing protein -
  LAJ50_RS05595 (LAJ50_05600) proC 1263992..1264843 (-) 852 WP_138654989.1 pyrroline-5-carboxylate reductase -
  LAJ50_RS05600 (LAJ50_05605) - 1265034..1265678 (-) 645 WP_138655004.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAJ50_RS05605 (LAJ50_05610) pilT 1265816..1266853 (+) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  LAJ50_RS05610 (LAJ50_05615) pilU 1266972..1268102 (+) 1131 WP_130550926.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LAJ50_RS05615 (LAJ50_05620) - 1268137..1269054 (+) 918 WP_224096490.1 RHS repeat-associated core domain-containing protein -
  LAJ50_RS05620 (LAJ50_05625) - 1269051..1269476 (+) 426 WP_138654993.1 hypothetical protein -
  LAJ50_RS05625 (LAJ50_05630) - 1269676..1270371 (+) 696 WP_171044675.1 YitT family protein -
  LAJ50_RS05630 (LAJ50_05635) - 1270505..1271371 (-) 867 WP_138654995.1 DUF72 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38709.43 Da        Isoelectric Point: 6.7269

>NTDB_id=605442 LAJ50_RS05605 WP_130517185.1 1265816..1266853(+) (pilT) [Pseudoxanthomonas sp. X-1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREMVQQPQGLILVTGPTGSGKSTTLAAMIDYINKSEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSQITRHQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=605442 LAJ50_RS05605 WP_130517185.1 1265816..1266853(+) (pilT) [Pseudoxanthomonas sp. X-1]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTCAAGAACAAGGCCTCGGACCTGCATCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTGGATGGCGACGTGCGTCGCATCAACATCCCCGCCCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAGTTCCTCGAGGTCGACTTCTCCTTCGAGATCCCCTCGCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGCACCATCCCGTCCGAGGTGCTGACCCTGGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAGATGGTGCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAGCGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAATGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATCAGCCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTC
CAGGACCTGGTCAAGCGCAGCCAGATCACGCGCCACCAGGCCAAGGAATACGCCAAGGACAAGCGGTTGTTTGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.779

99.71

0.786
  pilT Acinetobacter baumannii D1279779

78.779

99.71

0.786
  pilT Acinetobacter baumannii strain A118

78.779

99.71

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.103

98.841

0.643
  pilT Neisseria gonorrhoeae MS11

64.809

98.841

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.118

98.261

0.394
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391