Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LAJ50_RS05610 Genome accession   NZ_CP083376
Coordinates   1266972..1268102 (+) Length   376 a.a.
NCBI ID   WP_130550926.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. X-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1261972..1273102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAJ50_RS05585 (LAJ50_05590) soxR 1263062..1263496 (+) 435 WP_138654985.1 redox-sensitive transcriptional activator SoxR -
  LAJ50_RS05590 (LAJ50_05595) - 1263516..1263986 (-) 471 WP_138654987.1 DUF4426 domain-containing protein -
  LAJ50_RS05595 (LAJ50_05600) proC 1263992..1264843 (-) 852 WP_138654989.1 pyrroline-5-carboxylate reductase -
  LAJ50_RS05600 (LAJ50_05605) - 1265034..1265678 (-) 645 WP_138655004.1 YggS family pyridoxal phosphate-dependent enzyme -
  LAJ50_RS05605 (LAJ50_05610) pilT 1265816..1266853 (+) 1038 WP_130517185.1 type IV pilus twitching motility protein PilT Machinery gene
  LAJ50_RS05610 (LAJ50_05615) pilU 1266972..1268102 (+) 1131 WP_130550926.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LAJ50_RS05615 (LAJ50_05620) - 1268137..1269054 (+) 918 WP_224096490.1 RHS repeat-associated core domain-containing protein -
  LAJ50_RS05620 (LAJ50_05625) - 1269051..1269476 (+) 426 WP_138654993.1 hypothetical protein -
  LAJ50_RS05625 (LAJ50_05630) - 1269676..1270371 (+) 696 WP_171044675.1 YitT family protein -
  LAJ50_RS05630 (LAJ50_05635) - 1270505..1271371 (-) 867 WP_138654995.1 DUF72 domain-containing protein -
  LAJ50_RS05635 (LAJ50_05640) - 1271680..1272249 (-) 570 WP_138654997.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41862.17 Da        Isoelectric Point: 6.8287

>NTDB_id=605443 LAJ50_RS05610 WP_130550926.1 1266972..1268102(+) (pilU) [Pseudoxanthomonas sp. X-1]
MSAIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKLTPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLVPTPDGKARRVAMEIMLGTPLIQDYIREGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=605443 LAJ50_RS05610 WP_130550926.1 1266972..1268102(+) (pilU) [Pseudoxanthomonas sp. X-1]
ATGAGCGCCATCGACTTCACTTCGTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCGGGCAT
GCCGCCGTCGATCAAGGTCCACGGCAAGCTCACCCCGATCACGCAGACGCCGCTGACGCCGCAGCAGTCGCGCGACCTGG
TGCTCAACGTCATGACCCCCGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGCGTCGATGAGCTCAACCTGCCGCCGGTCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATTTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAGACCGGCCACCTGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGCCGCAGCCAGCTGCTGATGGACCTCTCGCTCAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGGTGCCGACCCCGGACGGCAAGGCGCGCCGGGTGGCGATGGAGATCATGCTGGGCACGCCGCTGATCC
AGGACTACATCCGCGAGGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCGCACCCTGGCCCAGGGGCTGGACGGCGTCGAGGTCGCCG
AGGCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.714

93.085

0.388
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain Lp02

41.194

89.096

0.367
  pilT Legionella pneumophila strain ERS1305867

41.194

89.096

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364