Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8U54_RS11455 Genome accession   NZ_CP082928
Coordinates   2542979..2544013 (+) Length   344 a.a.
NCBI ID   WP_070885862.1    Uniprot ID   -
Organism   Pseudomonas fulva strain ZJU1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537979..2549013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U54_RS11430 (K8U54_11410) - 2538166..2540148 (-) 1983 WP_249910223.1 dynamin-like GTPase family protein -
  K8U54_RS11435 (K8U54_11415) - 2540257..2540553 (-) 297 WP_249910224.1 DUF167 domain-containing protein -
  K8U54_RS11440 (K8U54_11420) - 2540699..2541289 (-) 591 WP_111461365.1 YggT family protein -
  K8U54_RS11445 (K8U54_11425) proC 2541301..2542119 (-) 819 WP_249910225.1 pyrroline-5-carboxylate reductase -
  K8U54_RS11450 (K8U54_11430) - 2542156..2542842 (-) 687 WP_249910226.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8U54_RS11455 (K8U54_11435) pilT 2542979..2544013 (+) 1035 WP_070885862.1 type IV pilus twitching motility protein PilT Machinery gene
  K8U54_RS11460 (K8U54_11440) pilU 2544079..2545224 (+) 1146 WP_070885863.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8U54_RS11465 (K8U54_11445) - 2545496..2546782 (-) 1287 WP_249910227.1 HipA domain-containing protein -
  K8U54_RS11470 (K8U54_11450) - 2546779..2547030 (-) 252 WP_249910228.1 helix-turn-helix domain-containing protein -
  K8U54_RS11475 (K8U54_11455) - 2547157..2547459 (-) 303 WP_249910229.1 hypothetical protein -
  K8U54_RS11480 (K8U54_11460) - 2547686..2548543 (-) 858 WP_249910230.1 TIGR03915 family putative DNA repair protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37965.66 Da        Isoelectric Point: 6.8893

>NTDB_id=603659 K8U54_RS11455 WP_070885862.1 2542979..2544013(+) (pilT) [Pseudomonas fulva strain ZJU1]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLSMEDLGMGEIFKKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMETLDACL
KRLVSKGLVSRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=603659 K8U54_RS11455 WP_070885862.1 2542979..2544013(+) (pilT) [Pseudomonas fulva strain ZJU1]
ATGGATATCACCGAGCTGCTCGCCTTCAGCGCCAAGCAAGGCGCGTCGGACCTGCACCTTTCCGCAGGCTTGCCGCCGAT
GATCCGTGTCGACGGCGATGTCCGCCGGATCAACCTGCCTGCCCTGGATCACAAGCAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAAGAGTTCCTGGAGACCGACTTCTCCTTCGAAGTGCCGGGCGTTGCGCGC
TTTCGGGTCAACGCCTTCAACCAGAACCGCGGCAGCGGTGCGGTGTTCCGGACCATTCCCTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGAGATCTTCAAGAAGATCTCCGACGTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCGACCGGTT
CGGGCAAGTCCACCACCCTGGCGGCGATGCTCGACTACATCAACAGCAACAAGTACCACCACATTCTCACCGTCGAAGAC
CCGATCGAATTCGTTCACGAATCGAAGAAGTGCCTGATCAACCAGCGTGAAGTGCATCGCGATACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCACTGCGTGAAGACCCGGACATCATTCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACTTCCGCCGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCCGAGGAGAAGTCCATGGTCCGTTCCATGCTCTCCGAGTCGCTGCAGGCGGTGATCTCCCAGAC
CCTGTTGAAGAAGATCGGCGGCGGTCGGGTAGCGGCCCACGAAATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACCGGCGGTGCGCTGGGCATGGAAACCCTGGATGCGTGCCTG
AAGAGGCTGGTTTCCAAAGGCCTGGTCAGCCGCGAAAGTGCCCGAGAAAAAGCCAAGACCCCGGAAAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

92.733

100

0.927
  pilT Pseudomonas stutzeri DSM 10701

92.733

100

0.927
  pilT Acinetobacter nosocomialis M2

86.047

100

0.86
  pilT Acinetobacter baumannii D1279779

85.756

100

0.858
  pilT Acinetobacter baumannii strain A118

85.756

100

0.858
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Vibrio cholerae strain A1552

39.71

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.701

97.384

0.387