Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8U54_RS11460 Genome accession   NZ_CP082928
Coordinates   2544079..2545224 (+) Length   381 a.a.
NCBI ID   WP_070885863.1    Uniprot ID   A0A2W7IRK5
Organism   Pseudomonas fulva strain ZJU1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2539079..2550224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U54_RS11435 (K8U54_11415) - 2540257..2540553 (-) 297 WP_249910224.1 DUF167 domain-containing protein -
  K8U54_RS11440 (K8U54_11420) - 2540699..2541289 (-) 591 WP_111461365.1 YggT family protein -
  K8U54_RS11445 (K8U54_11425) proC 2541301..2542119 (-) 819 WP_249910225.1 pyrroline-5-carboxylate reductase -
  K8U54_RS11450 (K8U54_11430) - 2542156..2542842 (-) 687 WP_249910226.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8U54_RS11455 (K8U54_11435) pilT 2542979..2544013 (+) 1035 WP_070885862.1 type IV pilus twitching motility protein PilT Machinery gene
  K8U54_RS11460 (K8U54_11440) pilU 2544079..2545224 (+) 1146 WP_070885863.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8U54_RS11465 (K8U54_11445) - 2545496..2546782 (-) 1287 WP_249910227.1 HipA domain-containing protein -
  K8U54_RS11470 (K8U54_11450) - 2546779..2547030 (-) 252 WP_249910228.1 helix-turn-helix domain-containing protein -
  K8U54_RS11475 (K8U54_11455) - 2547157..2547459 (-) 303 WP_249910229.1 hypothetical protein -
  K8U54_RS11480 (K8U54_11460) - 2547686..2548543 (-) 858 WP_249910230.1 TIGR03915 family putative DNA repair protein -
  K8U54_RS11485 (K8U54_11465) - 2548543..2549754 (-) 1212 WP_249910231.1 putative DNA modification/repair radical SAM protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42536.75 Da        Isoelectric Point: 6.7257

>NTDB_id=603660 K8U54_RS11460 WP_070885863.1 2544079..2545224(+) (pilU) [Pseudomonas fulva strain ZJU1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKTPLSPEMTRETVHGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKALMKRSTEHGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTSVSQGLSLEISDEDQGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=603660 K8U54_RS11460 WP_070885863.1 2544079..2545224(+) (pilU) [Pseudomonas fulva strain ZJU1]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTCGAGAAGGGCGGCTCCGACCTGTTCATCACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGACCAAGACCCCGCTGTCGCCGGAAATGACCCGTGAGACCGTGCACGGGG
TGATGAACGAGCAGCAGCGCCGGGATTTTGCCGAGAACCATGAGTGCAACTTCGCGATCAGCGCCCGTGGCATCGGTCGT
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGCCGTATCGAGACCAACATCCCGACGCTGGA
CGACCTCAAGCTGCCGGAGATTCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGTA
CCGGCAAGTCCACTTCCCTGGCGGCGATGATCGGTTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAGTACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCGACTCCTTCGAAGT
GGCGCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATTCTGATCGGTGAGGTGCGTACTCGCGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACTCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGCGGATCGGCAGAATCAGGTATGGATGGACCTGTCCCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGATCCCCACGCCCGATGGCAAGGGCCGCCGTGCGGTGATCGAGGTGCTGATCAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGCGCTGATGAAGCGTTCCACCGAGCACGGCATGCAGACCTTCGACCAGGCG
CTCTACAACCTCTACAGCCAGGGCGAGATCACCTACGAAGACGCCCTGCTGTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGATCGGAAACCGATGGTGAGCACCTGACCAGCGTTTCCCAGGGGCTGTCACTGGAAATCAGCGACG
AGGATCAGGGCCGGCGCTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7IRK5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

94.226

100

0.942
  pilU Acinetobacter baylyi ADP1

65.546

93.701

0.614
  pilU Vibrio cholerae strain A1552

58.857

91.864

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.452

88.189

0.383
  pilT Acinetobacter nosocomialis M2

41.194

87.927

0.362
  pilT Legionella pneumophila strain Lp02

41.194

87.927

0.362
  pilT Legionella pneumophila strain ERS1305867

41.194

87.927

0.362