Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8B83_RS17910 Genome accession   NZ_CP082926
Coordinates   4018038..4019075 (-) Length   345 a.a.
NCBI ID   WP_188739329.1    Uniprot ID   -
Organism   Shewanella inventionis strain D1489     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4013038..4024075
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B83_RS17885 (K8B83_17785) yciH 4013040..4013369 (-) 330 WP_188739378.1 stress response translation initiation inhibitor YciH -
  K8B83_RS17890 (K8B83_17790) hemH 4013827..4014840 (-) 1014 WP_188739333.1 ferrochelatase -
  K8B83_RS17895 (K8B83_17795) - 4015038..4015571 (-) 534 WP_188739332.1 glutathione peroxidase -
  K8B83_RS17900 (K8B83_17800) - 4015692..4016792 (-) 1101 WP_188739331.1 hypothetical protein -
  K8B83_RS17905 (K8B83_17805) pilU 4016914..4018026 (-) 1113 WP_188739330.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8B83_RS17910 (K8B83_17810) pilT 4018038..4019075 (-) 1038 WP_188739329.1 type IV pilus twitching motility protein PilT Machinery gene
  K8B83_RS17915 (K8B83_17815) - 4019103..4019789 (+) 687 WP_188739328.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8B83_RS17920 (K8B83_17820) proC 4020336..4021154 (+) 819 WP_188739327.1 pyrroline-5-carboxylate reductase -
  K8B83_RS17925 (K8B83_17825) - 4021305..4021853 (+) 549 WP_188739326.1 YggT family protein -
  K8B83_RS17930 (K8B83_17830) - 4021986..4022387 (+) 402 WP_223420107.1 DUF4426 domain-containing protein -
  K8B83_RS17935 (K8B83_17835) rdgB 4022715..4023359 (+) 645 WP_188739324.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38355.98 Da        Isoelectric Point: 6.9096

>NTDB_id=603643 K8B83_RS17910 WP_188739329.1 4018038..4019075(-) (pilT) [Shewanella inventionis strain D1489]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQARGAGAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAGMVDYINENRHEHILTIED
PIEFVHQNKQCLVNQREVHKHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMQKSSNKQANF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=603643 K8B83_RS17910 WP_188739329.1 4018038..4019075(-) (pilT) [Shewanella inventionis strain D1489]
ATGGAAATCACTGAATTACTCGCTTTTAGTGTAAAACACAAAGCATCAGATCTACACCTTTCTGCAGGTGTATCTCCTAT
GATCCGTGTCGACGGTGAAGTGCGAAAAATTAACTTACCCGCACTCGATCATCAAGGTGTTCATAGTCTGGTTTACGACA
TCATGAACGATAAGCAACGAAAAGACTACGAAGAACACCTCGAAATCGACTTTTCATTTGAAGTGCCTAACCTTGCTCGT
TTTCGTGTTAACGCGTTTAATCAGGCTCGCGGTGCTGGCGCTGTATTTCGTACCATTCCGTCAGATATTTTATCGTTAGA
GCAACTTGGTGCCCCTGAGATTTTCAAAAAAATATCCAGTTTTCCACGTGGATTAGTGCTAGTAACAGGGCCAACTGGTT
CGGGTAAATCAACAACCCTTGCAGGCATGGTCGACTATATTAATGAAAACCGTCACGAACACATTTTAACCATTGAAGAT
CCTATCGAATTCGTTCACCAAAATAAACAATGCTTAGTTAACCAACGTGAAGTTCACAAACACACCCACAGCTTCAATGC
TGCCCTAAGAAGTGCATTGCGTGAAGACCCTGACGTTATCCTCGTTGGTGAGATGCGTGACCTTGAAACCATTCGATTAG
CGATGACCGCAGCCGAAACCGGTCACTTAGTTTTTGGTACCTTGCACACCACCTCGGCGGCTAAAACCATTGACCGTATC
GTTGACGTTTTCCCTGAAGGTGAAAAGAGTATGGTACGAACCATGCTGTCTGAATCGCTTCAGGCTGTTATTTCACAAAC
CTTAATTAAAAAAATTGGTGGTGGCCGAGTTGCTGCTCACGAAATCATGATGGGTACACCGGCAATTCGTAACCTTATCC
GTGAAGATAAAGTCGCGCAAATGTACTCTGCCATTCAAACGGGTATGGCACATGGTATGCAAACGCTAGATCAATGTTTG
CAAAATCTAGTTAATCGCGGGCTCATTACTCGTGAAGATGCAATGCAAAAGAGCTCGAATAAACAAGCTAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.24

99.13

0.786
  pilT Acinetobacter baumannii D1279779

79.24

99.13

0.786
  pilT Acinetobacter baumannii strain A118

79.24

99.13

0.786
  pilT Pseudomonas aeruginosa PAK

78.994

97.971

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

75.66

98.841

0.748
  pilT Legionella pneumophila strain ERS1305867

75.66

98.841

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.81

97.681

0.701
  pilT Vibrio cholerae strain A1552

71.81

97.681

0.701
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.262

97.391

0.412
  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409
  pilU Acinetobacter baylyi ADP1

39.941

97.971

0.391