Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8B83_RS17905 Genome accession   NZ_CP082926
Coordinates   4016914..4018026 (-) Length   370 a.a.
NCBI ID   WP_188739330.1    Uniprot ID   -
Organism   Shewanella inventionis strain D1489     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4011914..4023026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B83_RS17880 (K8B83_17780) - 4012451..4013011 (-) 561 WP_188739334.1 YqgE/AlgH family protein -
  K8B83_RS17885 (K8B83_17785) yciH 4013040..4013369 (-) 330 WP_188739378.1 stress response translation initiation inhibitor YciH -
  K8B83_RS17890 (K8B83_17790) hemH 4013827..4014840 (-) 1014 WP_188739333.1 ferrochelatase -
  K8B83_RS17895 (K8B83_17795) - 4015038..4015571 (-) 534 WP_188739332.1 glutathione peroxidase -
  K8B83_RS17900 (K8B83_17800) - 4015692..4016792 (-) 1101 WP_188739331.1 hypothetical protein -
  K8B83_RS17905 (K8B83_17805) pilU 4016914..4018026 (-) 1113 WP_188739330.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8B83_RS17910 (K8B83_17810) pilT 4018038..4019075 (-) 1038 WP_188739329.1 type IV pilus twitching motility protein PilT Machinery gene
  K8B83_RS17915 (K8B83_17815) - 4019103..4019789 (+) 687 WP_188739328.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8B83_RS17920 (K8B83_17820) proC 4020336..4021154 (+) 819 WP_188739327.1 pyrroline-5-carboxylate reductase -
  K8B83_RS17925 (K8B83_17825) - 4021305..4021853 (+) 549 WP_188739326.1 YggT family protein -
  K8B83_RS17930 (K8B83_17830) - 4021986..4022387 (+) 402 WP_223420107.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40859.90 Da        Isoelectric Point: 6.2349

>NTDB_id=603642 K8B83_RS17905 WP_188739330.1 4016914..4018026(-) (pilU) [Shewanella inventionis strain D1489]
MDVRPFLNIMVEKKASDLFITAGFPPSAKIDGELRQLSETKLTPQQSLAFVESLMSDVQKKEFHDTSECNFAFAAKELGR
FRVSAFWQRESAGCVMRRIETNIPEVDDLKLPAILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTDSFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHNQLLFDLSLNLRGIVAQQLIPKSDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMGKSREQGMQTFDQA
LLDLYLEGEISYADALHHADSPNDLRLMIKLQGNEAASAGTMEGITLDMG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=603642 K8B83_RS17905 WP_188739330.1 4016914..4018026(-) (pilU) [Shewanella inventionis strain D1489]
ATGGATGTTCGTCCGTTTTTAAATATTATGGTCGAGAAAAAGGCCTCAGATTTATTTATTACTGCCGGTTTTCCGCCAAG
TGCCAAAATTGATGGTGAGTTACGTCAGTTATCAGAAACCAAATTAACACCACAGCAGTCTTTAGCTTTTGTTGAGTCGC
TAATGTCTGACGTACAGAAAAAAGAGTTTCATGACACCAGTGAGTGTAACTTTGCATTTGCGGCCAAAGAGCTTGGCCGT
TTTCGTGTAAGTGCCTTCTGGCAACGAGAGTCTGCTGGCTGTGTTATGCGTCGTATTGAAACCAATATTCCAGAAGTTGA
CGACTTAAAGCTGCCTGCCATTCTTAAAGATTTAGTCATGAGTAAGCGTGGCCTAATCATTATGGTAGGTGGGACAGGTA
CAGGTAAATCAACATCACTTGCCGCGCTGGTAGGTTACCGCAACGCCCACGCTCGCGGTCATATTTTAACCATTGAAGAT
CCAGTCGAGTTTGTTCATAACCACCGCAAAAGTATTATTACTCAGCGCGAAGTGGGTATCGACACTGACTCATTTGATGC
GGCACTGAAAAGCTCACTGCGCCAAGCGCCCGATGTTATCTTAATTGGTGAAATCCGAACCCAAGAAACAATGGAGTTTG
CGTTATCCTTTGCTGAAACTGGCCATTTATGTATGGCAACATTGCACGCAAACAACGCCAACCAAGCACTAGACCGTATT
ATGCATTTAGTGCCAGAGAGTAAGCACAATCAATTACTGTTCGATTTGTCGTTAAACCTACGCGGCATCGTGGCACAGCA
ACTTATCCCTAAATCTGACGGTACAGGCCGCCGCGCAGCAATTGAAGTATTAATTAACACCCCCCGTGTTGCGAGCCTTA
TTGCTAAAAACGAGCTGCATTTACTCAAAGAAACCATGGGTAAATCTCGCGAGCAGGGTATGCAAACGTTTGACCAAGCG
TTATTAGACTTATACCTCGAAGGCGAAATCAGTTACGCAGATGCATTGCACCACGCCGACTCGCCAAACGATTTACGTCT
CATGATTAAATTGCAAGGTAACGAAGCCGCATCAGCAGGAACGATGGAAGGCATAACCTTGGATATGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.022

100

0.614
  pilU Acinetobacter baylyi ADP1

61.364

95.135

0.584
  pilU Vibrio cholerae strain A1552

56.64

99.73

0.565
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

89.189

0.376
  pilT Vibrio cholerae strain A1552

42.121

89.189

0.376
  pilT Legionella pneumophila strain Lp02

41.39

89.459

0.37
  pilT Legionella pneumophila strain ERS1305867

41.39

89.459

0.37
  pilT Acinetobacter baumannii D1279779

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.474

92.432

0.365
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Pseudomonas aeruginosa PAK

38.841

93.243

0.362