Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K8O84_RS05085 Genome accession   NZ_CP082862
Coordinates   1053648..1054691 (-) Length   347 a.a.
NCBI ID   WP_061955519.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_1472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1048648..1059691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O84_RS05055 (K8O84_05025) - 1049555..1049935 (+) 381 WP_150979578.1 CidA/LrgA family protein -
  K8O84_RS05060 (K8O84_05030) - 1049932..1050657 (+) 726 WP_150979577.1 LrgB family protein -
  K8O84_RS05065 (K8O84_05035) - 1050742..1051122 (+) 381 WP_061960251.1 VOC family protein -
  K8O84_RS05070 (K8O84_05040) - 1051156..1051848 (+) 693 WP_150979576.1 YafY family protein -
  K8O84_RS05075 (K8O84_05045) - 1051865..1052359 (-) 495 WP_150979575.1 glutathione peroxidase -
  K8O84_RS05080 (K8O84_05050) pilU 1052477..1053616 (-) 1140 WP_061955521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8O84_RS05085 (K8O84_05055) pilT 1053648..1054691 (-) 1044 WP_061955519.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O84_RS05090 (K8O84_05060) - 1054756..1055445 (+) 690 WP_150979673.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O84_RS05095 (K8O84_05065) proC 1055536..1056354 (+) 819 WP_150979574.1 pyrroline-5-carboxylate reductase -
  K8O84_RS05100 (K8O84_05070) ubiA 1056452..1057336 (-) 885 WP_150979573.1 4-hydroxybenzoate octaprenyltransferase -
  K8O84_RS05105 (K8O84_05075) - 1057448..1057933 (-) 486 WP_061955510.1 Dps family protein -
  K8O84_RS05110 (K8O84_05080) - 1058019..1058222 (-) 204 WP_150979572.1 hypothetical protein -
  K8O84_RS05115 (K8O84_05085) - 1058434..1059390 (-) 957 WP_061955506.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38343.13 Da        Isoelectric Point: 6.8705

>NTDB_id=603220 K8O84_RS05085 WP_061955519.1 1053648..1054691(-) (pilT) [Cupriavidus pauculus strain FDAARGOS_1472]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMTHKDVHAMVYDIMTDVQRKSYEERLEIDFSFEISGLSR
FRVNAYTTQRGAAAVFRTIPSKVLTLDDLHAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHTSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGVINFNDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=603220 K8O84_RS05085 WP_061955519.1 1053648..1054691(-) (pilT) [Cupriavidus pauculus strain FDAARGOS_1472]
ATGGACATCGCACAGCTATTGGCCTTTGCGGTCAAGAACAAGGCCTCCGATCTTCACCTGTCGGCCGACATGCCGCCGAT
GGTGCGTATCCACGGGGATATGCGGCGCATCAACGTCGCGGCCATGACGCACAAGGACGTGCACGCCATGGTGTACGACA
TCATGACCGACGTGCAGCGCAAGAGTTACGAAGAGCGGCTGGAAATCGATTTCTCGTTCGAGATTTCCGGGCTCTCCCGC
TTCCGCGTCAATGCGTACACCACGCAGCGCGGCGCGGCCGCGGTGTTCCGGACGATTCCTTCCAAGGTGCTGACGCTGGA
CGACCTCCACGCGCCGGCCGTGTTCGCCGACCTGTGCATGAAGCCGCGCGGGTTGGTGCTGGTGACGGGCCCGACAGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGACCATCGCAACGACAACGATATGGGCCACATCCTCACGGTTGAAGAC
CCAATCGAATTCGTGCACACCTCGAAGAAAAGCCTGATCAACCAGCGCGAGCTGGGCCCGCATACACATTCGTTTGCCAA
CGCGCTGCGCTCGGCGTTGCGTGAGGATCCAGACGTGGTGCTGGTCGGCGAATTGCGCGACCTTGAAACGATCCGCCTCG
CGCTGACTGCGGCCGAAACCGGCCACCTGGTGTTTGCCACGCTGCACACCAGTTCCGCGGCCAAGACCATCGACCGTGTG
GTCGACGTGTTTCCGCCCGAAGAAAAGGATATGGTGCGCACCATGTTGTCCGAATCGCTCGAAGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCAACGGACGCACCGCTGCGCACGAGATCATGATCGCCACGCCCGCGATCCGGCATCTGA
TCCGCGAGAACAAGATTGCGCAGATGTATTCGATGATGCAGACGTCGAGCGGCCTTGGCATGCAGACGCTCGATCAGTGC
CTGAGCGATCTGATCAAGCGCGGCGTGATCAACTTCAACGATGCCCGGGCAATTGCCAAGAACCCCGACGCCTTCATGGG
TTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.098

99.712

0.709
  pilT Acinetobacter baumannii D1279779

71.098

99.712

0.709
  pilT Acinetobacter baumannii strain A118

71.098

99.712

0.709
  pilT Pseudomonas stutzeri DSM 10701

69.275

99.424

0.689
  pilT Acinetobacter baylyi ADP1

68.986

99.424

0.686
  pilT Pseudomonas aeruginosa PAK

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

65.797

99.424

0.654
  pilT Neisseria meningitidis 8013

65.797

99.424

0.654
  pilT Legionella pneumophila strain Lp02

65.507

99.424

0.651
  pilT Legionella pneumophila strain ERS1305867

65.507

99.424

0.651
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.579

97.118

0.637
  pilT Vibrio cholerae strain A1552

65.579

97.118

0.637
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.406

99.424

0.481
  pilU Acinetobacter baylyi ADP1

40.698

99.135

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

39.701

96.542

0.383