Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K8O84_RS05080 Genome accession   NZ_CP082862
Coordinates   1052477..1053616 (-) Length   379 a.a.
NCBI ID   WP_061955521.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_1472     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047477..1058616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O84_RS05050 (K8O84_05020) glcF 1048200..1049471 (+) 1272 WP_150979579.1 glycolate oxidase subunit GlcF -
  K8O84_RS05055 (K8O84_05025) - 1049555..1049935 (+) 381 WP_150979578.1 CidA/LrgA family protein -
  K8O84_RS05060 (K8O84_05030) - 1049932..1050657 (+) 726 WP_150979577.1 LrgB family protein -
  K8O84_RS05065 (K8O84_05035) - 1050742..1051122 (+) 381 WP_061960251.1 VOC family protein -
  K8O84_RS05070 (K8O84_05040) - 1051156..1051848 (+) 693 WP_150979576.1 YafY family protein -
  K8O84_RS05075 (K8O84_05045) - 1051865..1052359 (-) 495 WP_150979575.1 glutathione peroxidase -
  K8O84_RS05080 (K8O84_05050) pilU 1052477..1053616 (-) 1140 WP_061955521.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K8O84_RS05085 (K8O84_05055) pilT 1053648..1054691 (-) 1044 WP_061955519.1 type IV pilus twitching motility protein PilT Machinery gene
  K8O84_RS05090 (K8O84_05060) - 1054756..1055445 (+) 690 WP_150979673.1 YggS family pyridoxal phosphate-dependent enzyme -
  K8O84_RS05095 (K8O84_05065) proC 1055536..1056354 (+) 819 WP_150979574.1 pyrroline-5-carboxylate reductase -
  K8O84_RS05100 (K8O84_05070) ubiA 1056452..1057336 (-) 885 WP_150979573.1 4-hydroxybenzoate octaprenyltransferase -
  K8O84_RS05105 (K8O84_05075) - 1057448..1057933 (-) 486 WP_061955510.1 Dps family protein -
  K8O84_RS05110 (K8O84_05080) - 1058019..1058222 (-) 204 WP_150979572.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42494.69 Da        Isoelectric Point: 6.4437

>NTDB_id=603219 K8O84_RS05080 WP_061955521.1 1052477..1053616(-) (pilU) [Cupriavidus pauculus strain FDAARGOS_1472]
MLDRESASKYINDLLELMVNNRGSDLFITSEFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDTRECNFA
ITAPNAGRFRVSAFIQQGRAGMVVRTINTRIPSVEDLDLPSSLHEIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTDSWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMISQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFELYESEKITYEDALKNADSLNDLRLMIKLHSVRHRDTDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=603219 K8O84_RS05080 WP_061955521.1 1052477..1053616(-) (pilU) [Cupriavidus pauculus strain FDAARGOS_1472]
ATGCTCGACCGTGAGTCCGCATCGAAGTACATCAACGACCTGCTCGAACTGATGGTGAACAACCGCGGTTCGGACCTGTT
CATCACGTCGGAATTTCCGCCGGCCATCAAGGTCGACGGCAAGATTACGCCGGTCTCGCAGCAGCCGCTCAATCCCACGC
AGGCGCTTGGCCTGGTGCGTTCGATCATGAATGAGCGCCAGGTGAAGGAGTTCGACGACACGCGCGAATGCAACTTCGCC
ATCACCGCGCCCAATGCGGGACGCTTCCGCGTGTCGGCGTTCATCCAGCAGGGCCGCGCCGGCATGGTGGTGCGCACCAT
CAACACGCGCATTCCGTCCGTCGAGGATCTCGATCTGCCCAGCTCGCTGCATGAGATCGTGATGTCCAAGCGCGGTCTGG
TGATCGTCACCGGCGCGACGGGCTCGGGCAAGTCCACCTCGCTCGCGGCGATGCTCGATCACCGCAACGCGCATTCGTAC
GGCCACATCATCACGATCGAGGATCCGATCGAATACGTCCACGCGCATCAGAACTGCATCGTGACGCAGCGCGAAGTCGG
CATCGACACCGATTCCTGGCACGTCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGACCGCGAAACCATGGAGTACGCGATGCAATACGCGGAGACCGGACACCTCTGCCTGGCCACGCTGCACGCGAACAAC
GCCAACCAGGCGATCGATCGCATCGTCAACTTCTTCCCCGAGGAGAAGCGCCAGCAATTGCTGATCGATCTGTCGCTAAA
CCTGAAGGCAATGATTTCGCAGCGGCTGCTGCCGCGCGCTGGCCGCAAGGGCCGCGTGCCGGCCGTGGAAATCATGATCG
GCACGCCGCTGGTGGCCGATCTGATTTTCAAGGGCGAGATCCACGAGCTCAAGGAAGTCATCAAGAAATCGCGCGAGCAG
GGCATGATTTCGTTCGACCAGGCGCTGTTCGAACTTTATGAATCGGAAAAGATCACCTACGAGGATGCGCTCAAGAATGC
CGATTCGCTCAACGATTTGCGCCTGATGATCAAGCTGCATAGCGTGCGGCACCGCGATACCGATCTAGGCGCCGGCACCG
AGCACCTGAACGTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.793

91.821

0.586
  pilU Acinetobacter baylyi ADP1

59.429

92.348

0.549
  pilU Vibrio cholerae strain A1552

53.994

95.778

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

88.918

0.393
  pilT Legionella pneumophila strain ERS1305867

44.144

87.863

0.388
  pilT Legionella pneumophila strain Lp02

44.144

87.863

0.388
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baylyi ADP1

44.099

84.96

0.375
  pilT Neisseria meningitidis 8013

41.42

89.182

0.369
  pilT Acinetobacter baumannii D1279779

43.887

84.169

0.369
  pilT Acinetobacter nosocomialis M2

43.887

84.169

0.369
  pilT Acinetobacter baumannii strain A118

43.887

84.169

0.369
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361
  pilT Neisseria gonorrhoeae MS11

40.653

88.918

0.361