Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6749_RS13090 Genome accession   NZ_CP082310
Coordinates   2819816..2820856 (-) Length   346 a.a.
NCBI ID   WP_005436511.1    Uniprot ID   A0A0C1VE31
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2814816..2825856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13060 (K6749_12980) endA 2814960..2815655 (+) 696 WP_017635582.1 deoxyribonuclease I -
  K6749_RS13065 (K6749_12985) rsmE 2815769..2816500 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6749_RS13070 (K6749_12990) gshB 2816514..2817464 (+) 951 WP_005381021.1 glutathione synthase -
  K6749_RS13075 (K6749_12995) - 2817592..2818155 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  K6749_RS13080 (K6749_13000) ruvX 2818191..2818616 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  K6749_RS13085 (K6749_13005) pilU 2818678..2819784 (-) 1107 WP_238970103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6749_RS13090 (K6749_13010) pilT 2819816..2820856 (-) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  K6749_RS13095 (K6749_13015) - 2820885..2821586 (+) 702 WP_238970104.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6749_RS13100 (K6749_13020) proC 2821746..2822564 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  K6749_RS13105 (K6749_13025) - 2822617..2823174 (+) 558 WP_005381012.1 YggT family protein -
  K6749_RS13110 (K6749_13030) yggU 2823174..2823464 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  K6749_RS13115 (K6749_13035) - 2823593..2824024 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  K6749_RS13120 (K6749_13040) - 2824143..2824745 (+) 603 WP_042521298.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38661.14 Da        Isoelectric Point: 6.5043

>NTDB_id=601245 K6749_RS13090 WP_005436511.1 2819816..2820856(-) (pilT) [Vibrio alginolyticus strain HYV1]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGIPAFTHQEVHRLVFEIMNDAQRSEFEEKLEVDFSFELHNVGR
FRVNAFNQSRGCAAVFRTIPSSIPTLEELEAPEIFRKIANAEKGLVLVTGPTGSGKSTTLAAIVDYINRNHNKHILTIED
PIEFVHNNNKCLINQREVHRDTHSFQNALRSALREDPDVILVGEMRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRNGGGRIACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLMAQGMVSREEVDKKVEIEAQQFS

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=601245 K6749_RS13090 WP_005436511.1 2819816..2820856(-) (pilT) [Vibrio alginolyticus strain HYV1]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAATGCTTCGGATCTACATCTTTCTGCAGGTGTACCACCAAT
GGTTCGTATTGATGGTGATGTGAGAAAGCTGGGCATTCCAGCGTTTACACACCAAGAAGTGCACCGTTTGGTTTTTGAAA
TCATGAACGATGCGCAGCGCAGTGAGTTCGAAGAAAAACTCGAAGTCGATTTCTCATTTGAATTGCATAACGTTGGCCGT
TTTCGTGTCAACGCTTTCAACCAATCTCGCGGTTGTGCGGCTGTGTTCCGTACCATCCCAAGCAGTATTCCAACACTGGA
AGAGCTAGAAGCTCCTGAGATCTTCAGAAAAATCGCTAACGCCGAGAAAGGTCTGGTACTGGTTACTGGCCCAACGGGTT
CTGGTAAATCGACGACCTTGGCAGCCATTGTTGACTACATTAACCGTAATCACAATAAGCACATCCTAACAATTGAAGAT
CCAATTGAATTTGTTCACAACAACAATAAGTGTCTGATCAACCAACGTGAAGTACACCGTGATACACACAGCTTCCAAAA
CGCGCTGCGTAGCGCATTGCGTGAAGACCCGGATGTGATTCTCGTAGGTGAGATGCGTGACAAAGAAACCATCAGTCTAG
CGCTGACAGCGGCTGAAACCGGTCACCTTGTTTTCGGTACGTTGCACACGAGCTCTGCGGCGAAAACCATCGACCGTATT
ATCGACGTGTTCCCGGGGAGCGACAAAGACATGGTGCGCTCGATGTTGTCGGAATCTCTCCGTTCGGTTATCGCGCAAAA
GCTTTTGAAACGTAACGGCGGTGGTCGTATTGCGTGTCATGAAATCATGATGGCAACACCGGCGATCCGAAACCTTATTC
GTGAAGATAAAGTCGCGCAGATGTACTCGATCATTCAGACAGGTGCAGCTCACGGCATGCAGACCATGGAACAAAATGCT
CGTCAATTGATGGCGCAAGGCATGGTCTCGCGTGAAGAAGTGGACAAGAAAGTTGAAATTGAAGCGCAGCAATTCTCGTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C1VE31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.667

99.711

0.864
  pilT Vibrio cholerae strain A1552

86.667

99.711

0.864
  pilT Acinetobacter baumannii D1279779

72.424

95.376

0.691
  pilT Acinetobacter baumannii strain A118

72.424

95.376

0.691
  pilT Acinetobacter nosocomialis M2

72.121

95.376

0.688
  pilT Acinetobacter baylyi ADP1

71.818

95.376

0.685
  pilT Pseudomonas stutzeri DSM 10701

69.322

97.977

0.679
  pilT Pseudomonas aeruginosa PAK

69.118

98.266

0.679
  pilT Legionella pneumophila strain Lp02

67.976

95.665

0.65
  pilT Legionella pneumophila strain ERS1305867

67.976

95.665

0.65
  pilT Neisseria meningitidis 8013

64.412

98.266

0.633
  pilT Neisseria gonorrhoeae MS11

64.118

98.266

0.63
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

100

0.506
  pilU Vibrio cholerae strain A1552

41.916

96.532

0.405
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.532

0.393
  pilU Acinetobacter baylyi ADP1

39.062

92.486

0.361