Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6749_RS13085 Genome accession   NZ_CP082310
Coordinates   2818678..2819784 (-) Length   368 a.a.
NCBI ID   WP_238970103.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HYV1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2813678..2824784
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6749_RS13055 (K6749_12975) - 2814312..2814809 (+) 498 WP_054866486.1 SprT family zinc-dependent metalloprotease -
  K6749_RS13060 (K6749_12980) endA 2814960..2815655 (+) 696 WP_017635582.1 deoxyribonuclease I -
  K6749_RS13065 (K6749_12985) rsmE 2815769..2816500 (+) 732 WP_025768830.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6749_RS13070 (K6749_12990) gshB 2816514..2817464 (+) 951 WP_005381021.1 glutathione synthase -
  K6749_RS13075 (K6749_12995) - 2817592..2818155 (+) 564 WP_005381020.1 YqgE/AlgH family protein -
  K6749_RS13080 (K6749_13000) ruvX 2818191..2818616 (+) 426 WP_005381017.1 Holliday junction resolvase RuvX -
  K6749_RS13085 (K6749_13005) pilU 2818678..2819784 (-) 1107 WP_238970103.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6749_RS13090 (K6749_13010) pilT 2819816..2820856 (-) 1041 WP_005436511.1 type IV pilus twitching motility protein PilT Machinery gene
  K6749_RS13095 (K6749_13015) - 2820885..2821586 (+) 702 WP_238970104.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6749_RS13100 (K6749_13020) proC 2821746..2822564 (+) 819 WP_005381013.1 pyrroline-5-carboxylate reductase -
  K6749_RS13105 (K6749_13025) - 2822617..2823174 (+) 558 WP_005381012.1 YggT family protein -
  K6749_RS13110 (K6749_13030) yggU 2823174..2823464 (+) 291 WP_005393980.1 DUF167 family protein YggU -
  K6749_RS13115 (K6749_13035) - 2823593..2824024 (+) 432 WP_005381010.1 DUF4426 domain-containing protein -
  K6749_RS13120 (K6749_13040) - 2824143..2824745 (+) 603 WP_042521298.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41294.43 Da        Isoelectric Point: 6.7733

>NTDB_id=601244 K6749_RS13085 WP_238970103.1 2818678..2819784(-) (pilU) [Vibrio alginolyticus strain HYV1]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLTENDVAVLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNSNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKSTMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=601244 K6749_RS13085 WP_238970103.1 2818678..2819784(-) (pilU) [Vibrio alginolyticus strain HYV1]
ATGGATCTGAATAAATTTCTCGAAGGCATGTTGGCGTTAAAAGCGTCGGATCTCTACATCACTGTTGGTGCGCCGATCTT
ATTTCGTGTCGATGGCGAACTGCGTCCGCAAGGCGATAAGTTGACTGAAAATGATGTTGCTGTGTTACTCGATAGTGCGA
TGGATCCAGAGCGACGTCAGGAGTTTCGTAAAAGCCGTGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGTTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCTGGCGCAGTGATTCGTCGTATTGAAACTAATATTCCTACTTTTGAGCAGTT
AAAGTTACCGTTAGTGTTGCAAGATTTGGCGATAGCTAAGCGTGGTTTGGTGCTGGTGGTCGGTGCTACTGGATCGGGTA
AATCGACCACCATGGCGGCGATGACAGGCTATCGAAACAGCAATAAAACCGGGCACATTTTGACGGTCGAAGATCCGATC
GAATTCGTGCATGAACACAAGCGCTGTATCGTGACTCAACGCGAAGTTGGGTTAGATACGGAAAGCTATGAAGTTGCGCT
TAAAAACTCGCTGCGCCAAGCACCAGATATGATTTTGATTGGTGAGATCCGTAGCCGTGAAACAATGGAATACGCGATGA
CCTTCGCCGAAACGGGTCACCTGTGTATGGCAACGTTGCACGCCAATAATGCCAACCAAGCTTTAGAGCGCATTCTTCAC
TTGGTACCGAAAGATCAAAAAGAACAATTCTTGTTCGATTTGTCGATGAACCTAAAAGGCGTGGTTGGTCAGCAGTTGAT
CCGAGATAAAAATGGGCAGGGACGTCATGGTGTATTTGAGATCTTGCTAAATAGCCCACGTGTGTCGGATTTGATTCGTC
GCGGAGATTTACATGAGTTGAAGTCAACCATGGCTCGCTCAAATGAGTTTGGTATGCTGACGTTCGATCAATCTCTCTAT
AAGTTAGTGATGCAAGGTAAGATCAGCGAAGAGGATGCACTACATAGTGCCGACTCGGCAAATGATCTGCGCTTGATGTT
GAAAACGCAGCGTGGTGAAGCATTCTCTACGGGCAGTTTGGCAAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

58.571

95.109

0.557
  pilU Acinetobacter baylyi ADP1

55.271

95.38

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.615

88.315

0.394
  pilT Legionella pneumophila strain ERS1305867

42.478

92.12

0.391
  pilT Legionella pneumophila strain Lp02

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.617

90.761

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361