Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6Q96_RS02350 Genome accession   NZ_CP082275
Coordinates   474418..475455 (+) Length   345 a.a.
NCBI ID   WP_251877497.1    Uniprot ID   -
Organism   Grimontia kaedaensis strain 020920N     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469418..480455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6Q96_RS02315 (K6Q96_02315) hemW 469577..470728 (-) 1152 WP_251877485.1 radical SAM family heme chaperone HemW -
  K6Q96_RS02320 (K6Q96_02320) - 470735..471334 (-) 600 WP_251877487.1 XTP/dITP diphosphatase -
  K6Q96_RS02325 (K6Q96_02325) - 471423..471857 (-) 435 WP_251877489.1 DUF4426 domain-containing protein -
  K6Q96_RS02330 (K6Q96_02330) yggU 471931..472242 (-) 312 WP_251877491.1 DUF167 family protein YggU -
  K6Q96_RS02335 (K6Q96_02335) - 472246..472812 (-) 567 WP_002537485.1 YggT family protein -
  K6Q96_RS02340 (K6Q96_02340) proC 472822..473640 (-) 819 WP_251877493.1 pyrroline-5-carboxylate reductase -
  K6Q96_RS02345 (K6Q96_02345) - 473687..474391 (-) 705 WP_251877495.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6Q96_RS02350 (K6Q96_02350) pilT 474418..475455 (+) 1038 WP_251877497.1 type IV pilus twitching motility protein PilT Machinery gene
  K6Q96_RS02355 (K6Q96_02355) pilU 475458..476567 (+) 1110 WP_251877499.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6Q96_RS02360 (K6Q96_02360) ruvX 476617..477048 (-) 432 WP_062666139.1 Holliday junction resolvase RuvX -
  K6Q96_RS02365 (K6Q96_02365) - 477058..477621 (-) 564 WP_251877501.1 YqgE/AlgH family protein -
  K6Q96_RS02370 (K6Q96_02370) gshB 477732..478679 (-) 948 WP_251877503.1 glutathione synthase -
  K6Q96_RS02375 (K6Q96_02375) rsmE 478709..479440 (-) 732 WP_251877505.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6Q96_RS02380 (K6Q96_02380) - 479525..480232 (-) 708 WP_251877507.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38007.53 Da        Isoelectric Point: 6.5649

>NTDB_id=600796 K6Q96_RS02350 WP_251877497.1 474418..475455(+) (pilT) [Grimontia kaedaensis strain 020920N]
MDITELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLSLPPLDHNAVHRLIFDIMNDAQQREFEQNLEVDFSFELASVGR
FRVNAFHQSRGCAAVFRSIPSKIPTLDAINAPAVFQQFTEYQKGLVLVTGPTGSGKSTTLAAMIDKINSSQHRHVLTIED
PIEFVHEQKNCLINQREVHRDTHSFNNALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGADKPMVRSMLSESLRAVVSQKLMKQISGGRIAAHEVMIATPAIRNLIREDKIAQMYSMIQTGSGLGMLTMEQSL
RNLTMQGLVDTAEATKALDNAGMGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=600796 K6Q96_RS02350 WP_251877497.1 474418..475455(+) (pilT) [Grimontia kaedaensis strain 020920N]
ATGGATATTACTGAATTACTGGACTTTAGTGTAAAGCATAACGCTTCAGATCTACATCTTTCAGCTGGGGTTCCCCCAAT
GGTCCGCGTAGATGGTGATGTCCGCAAGCTTAGCCTGCCTCCGTTAGACCACAATGCGGTGCATCGTCTTATCTTCGATA
TCATGAACGATGCCCAGCAGCGAGAGTTTGAACAGAACCTCGAAGTCGATTTTTCTTTTGAGCTGGCGTCTGTCGGGCGT
TTCCGTGTGAATGCGTTTCATCAATCCCGTGGTTGTGCTGCGGTGTTCCGTAGCATTCCTTCGAAAATTCCGACACTTGA
TGCGATTAACGCACCAGCAGTGTTCCAACAATTTACCGAATATCAGAAAGGTTTGGTACTGGTCACCGGGCCGACAGGCT
CAGGTAAGTCGACCACGCTGGCGGCGATGATAGACAAAATTAACAGCAGTCAGCATCGTCACGTATTGACCATCGAAGAC
CCGATTGAATTTGTTCACGAACAGAAAAACTGTCTGATCAACCAGCGTGAAGTGCATCGCGATACCCACTCCTTCAACAA
TGCGCTACGCTCGGCACTGCGTGAAGACCCGGATGTGATTCTGGTTGGTGAGCTTCGTGACAAGGAAACCATTAGCCTTG
CACTGACGGCAGCAGAAACTGGTCACTTGGTATTTGGTACGTTGCACACTTCCAGCGCAGCAAAAACCATCGACCGCATT
ATTGATGTTTTTCCAGGTGCGGATAAGCCGATGGTGCGTTCCATGCTTTCTGAATCGCTGCGTGCGGTGGTTTCGCAAAA
ACTGATGAAGCAGATTTCCGGCGGCCGTATTGCTGCCCATGAGGTGATGATTGCGACACCTGCTATCCGAAACCTTATCC
GTGAAGACAAGATTGCCCAGATGTATTCGATGATCCAAACCGGATCAGGCCTTGGCATGCTGACGATGGAACAGTCGCTT
CGTAACCTCACCATGCAGGGGCTGGTGGATACTGCCGAAGCAACCAAAGCATTAGATAACGCAGGGATGGGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.631

98.261

0.733
  pilT Vibrio cholerae strain A1552

74.631

98.261

0.733
  pilT Pseudomonas aeruginosa PAK

68.862

96.812

0.667
  pilT Acinetobacter baumannii D1279779

67.953

97.681

0.664
  pilT Acinetobacter baumannii strain A118

67.953

97.681

0.664
  pilT Acinetobacter nosocomialis M2

67.953

97.681

0.664
  pilT Pseudomonas stutzeri DSM 10701

69.301

95.362

0.661
  pilT Acinetobacter baylyi ADP1

67.964

96.812

0.658
  pilT Legionella pneumophila strain Lp02

65.889

99.42

0.655
  pilT Legionella pneumophila strain ERS1305867

65.889

99.42

0.655
  pilT Neisseria meningitidis 8013

62.536

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.248

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.303

95.652

0.481
  pilU Acinetobacter baylyi ADP1

40.351

99.13

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Vibrio cholerae strain A1552

40.058

99.13

0.397