Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6Q96_RS02355 Genome accession   NZ_CP082275
Coordinates   475458..476567 (+) Length   369 a.a.
NCBI ID   WP_251877499.1    Uniprot ID   -
Organism   Grimontia kaedaensis strain 020920N     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 470458..481567
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6Q96_RS02320 (K6Q96_02320) - 470735..471334 (-) 600 WP_251877487.1 XTP/dITP diphosphatase -
  K6Q96_RS02325 (K6Q96_02325) - 471423..471857 (-) 435 WP_251877489.1 DUF4426 domain-containing protein -
  K6Q96_RS02330 (K6Q96_02330) yggU 471931..472242 (-) 312 WP_251877491.1 DUF167 family protein YggU -
  K6Q96_RS02335 (K6Q96_02335) - 472246..472812 (-) 567 WP_002537485.1 YggT family protein -
  K6Q96_RS02340 (K6Q96_02340) proC 472822..473640 (-) 819 WP_251877493.1 pyrroline-5-carboxylate reductase -
  K6Q96_RS02345 (K6Q96_02345) - 473687..474391 (-) 705 WP_251877495.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6Q96_RS02350 (K6Q96_02350) pilT 474418..475455 (+) 1038 WP_251877497.1 type IV pilus twitching motility protein PilT Machinery gene
  K6Q96_RS02355 (K6Q96_02355) pilU 475458..476567 (+) 1110 WP_251877499.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6Q96_RS02360 (K6Q96_02360) ruvX 476617..477048 (-) 432 WP_062666139.1 Holliday junction resolvase RuvX -
  K6Q96_RS02365 (K6Q96_02365) - 477058..477621 (-) 564 WP_251877501.1 YqgE/AlgH family protein -
  K6Q96_RS02370 (K6Q96_02370) gshB 477732..478679 (-) 948 WP_251877503.1 glutathione synthase -
  K6Q96_RS02375 (K6Q96_02375) rsmE 478709..479440 (-) 732 WP_251877505.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K6Q96_RS02380 (K6Q96_02380) - 479525..480232 (-) 708 WP_251877507.1 endonuclease -
  K6Q96_RS02385 (K6Q96_02385) - 480347..480838 (-) 492 WP_251877509.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41117.27 Da        Isoelectric Point: 6.3705

>NTDB_id=600797 K6Q96_RS02355 WP_251877499.1 475458..476567(+) (pilU) [Grimontia kaedaensis strain 020920N]
MQNTLQELMDKMLAQKASDCYITVDAPCMLRVDGSLKPVGGKLDLENVKALVAEAMDTETEQKFYADREANFALVTENGR
FRVSAFWQRDLPGMVIRRIETKIPDIYSLGLPDVIQDLSIAKRGLVLIVGATGSGKSTSMAAMVGFRNRHRSGHILTVED
PIEFIHPHDKCIVTQREIGIDTDSYEVALKNSLRQAPDMIMIGEIRTQETMEYAMQFAETGHLCIATLHANNANQALERV
LHLVPKERREQFLFDLSLNLRGILAQQLVPDVSGIGRHGVYELLLNTSHVSDLIRRGELHELKETIRKGKNAGMCTFDQS
LFELFQAGKVSKAEALHHADSRNDLKLMMKMAEQSSGGLSLANVSIEGQ

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=600797 K6Q96_RS02355 WP_251877499.1 475458..476567(+) (pilU) [Grimontia kaedaensis strain 020920N]
ATGCAGAACACGCTCCAGGAGCTTATGGATAAGATGCTGGCGCAGAAGGCATCGGATTGTTACATCACGGTCGATGCCCC
TTGTATGTTGCGTGTCGATGGCAGCTTAAAACCTGTGGGCGGAAAGCTCGACCTGGAAAATGTCAAAGCGCTGGTTGCTG
AGGCCATGGATACCGAAACCGAGCAGAAGTTTTATGCCGACCGTGAAGCGAATTTTGCCTTGGTGACTGAAAATGGACGC
TTCCGAGTCAGTGCGTTTTGGCAGCGTGATTTGCCAGGTATGGTTATTCGCCGCATTGAAACTAAAATCCCTGATATTTA
CTCACTGGGTTTGCCGGATGTGATTCAGGATTTGTCTATTGCCAAGCGTGGTCTGGTACTGATTGTTGGTGCGACAGGTT
CGGGTAAATCAACCTCAATGGCAGCGATGGTGGGGTTCCGCAACCGTCATCGCAGCGGCCATATTCTGACAGTGGAAGAC
CCGATTGAGTTTATCCATCCGCATGACAAATGCATCGTGACCCAGCGTGAAATTGGTATTGATACGGACAGCTACGAAGT
GGCGTTGAAAAACTCACTGCGTCAGGCGCCGGATATGATCATGATTGGTGAAATCCGAACCCAGGAAACTATGGAATATG
CCATGCAGTTTGCTGAAACCGGTCACCTTTGTATCGCAACCTTACACGCCAACAATGCCAATCAGGCACTGGAAAGGGTG
CTTCATTTGGTACCGAAAGAGCGTCGCGAACAATTCCTGTTTGATTTGTCGCTCAACCTTCGCGGTATTCTGGCCCAGCA
ACTGGTGCCAGACGTCAGTGGTATCGGACGTCATGGCGTATACGAGCTTCTGCTCAATACTTCCCATGTGTCGGATCTGA
TTCGCCGTGGTGAACTGCATGAGCTGAAAGAAACGATCCGTAAAGGCAAGAACGCGGGAATGTGTACCTTTGACCAATCC
CTGTTTGAGTTGTTTCAGGCGGGTAAGGTTTCTAAAGCCGAAGCACTCCACCACGCAGACTCGCGAAATGATCTCAAACT
CATGATGAAAATGGCCGAGCAGTCATCTGGCGGACTTTCGCTGGCGAATGTCAGCATTGAGGGGCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

65.659

98.645

0.648
  pilU Pseudomonas stutzeri DSM 10701

54.648

96.206

0.526
  pilU Acinetobacter baylyi ADP1

51.117

97.019

0.496
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.244

95.664

0.366