Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5L94_RS04950 Genome accession   NZ_CP082270
Coordinates   1056557..1057594 (+) Length   345 a.a.
NCBI ID   WP_006422551.1    Uniprot ID   -
Organism   Stenotrophomonas sp. DFS-20110405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1051557..1062594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5L94_RS04925 (K5L94_04925) soxR 1052817..1053251 (+) 435 WP_008267329.1 redox-sensitive transcriptional activator SoxR -
  K5L94_RS04930 (K5L94_04930) - 1053334..1053936 (-) 603 WP_274512072.1 short chain dehydrogenase -
  K5L94_RS04935 (K5L94_04935) - 1054037..1054912 (+) 876 WP_053508572.1 LysR family transcriptional regulator -
  K5L94_RS04940 (K5L94_04940) proC 1054952..1055773 (-) 822 WP_274512073.1 pyrroline-5-carboxylate reductase -
  K5L94_RS04945 (K5L94_04945) - 1055792..1056469 (-) 678 WP_274512074.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5L94_RS04950 (K5L94_04950) pilT 1056557..1057594 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  K5L94_RS04955 (K5L94_04955) pilU 1057673..1058836 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5L94_RS04960 (K5L94_04960) - 1059038..1060021 (+) 984 WP_274512075.1 aldo/keto reductase -
  K5L94_RS04965 (K5L94_04965) - 1060117..1061016 (-) 900 WP_180879644.1 LysR family transcriptional regulator -
  K5L94_RS04970 (K5L94_04970) - 1061342..1061554 (+) 213 WP_053448999.1 DUF4287 domain-containing protein -
  K5L94_RS04975 (K5L94_04975) - 1061666..1062373 (+) 708 WP_049456208.1 YitT family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38644.38 Da        Isoelectric Point: 6.6652

>NTDB_id=600663 K5L94_RS04950 WP_006422551.1 1056557..1057594(+) (pilT) [Stenotrophomonas sp. DFS-20110405]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQARDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=600663 K5L94_RS04950 WP_006422551.1 1056557..1057594(+) (pilT) [Stenotrophomonas sp. DFS-20110405]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCATTTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGGATCAACATTCCAGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCGTCGCTGGCGCGC
TTCCGTGTGAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTACACCGCGATACGCATGGCTTCAACGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCATACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATCTCGCAGGC
ACTGCTGAAGAAGGTGGGCGGCGGTCGTACCGCAGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGACTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

78.761

98.261

0.774
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

40.23

100

0.406
  pilU Vibrio cholerae strain A1552

40.23

100

0.406
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391