Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5L94_RS04955 Genome accession   NZ_CP082270
Coordinates   1057673..1058836 (+) Length   387 a.a.
NCBI ID   WP_049434422.1    Uniprot ID   A0AA43BPT5
Organism   Stenotrophomonas sp. DFS-20110405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1052673..1063836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5L94_RS04925 (K5L94_04925) soxR 1052817..1053251 (+) 435 WP_008267329.1 redox-sensitive transcriptional activator SoxR -
  K5L94_RS04930 (K5L94_04930) - 1053334..1053936 (-) 603 WP_274512072.1 short chain dehydrogenase -
  K5L94_RS04935 (K5L94_04935) - 1054037..1054912 (+) 876 WP_053508572.1 LysR family transcriptional regulator -
  K5L94_RS04940 (K5L94_04940) proC 1054952..1055773 (-) 822 WP_274512073.1 pyrroline-5-carboxylate reductase -
  K5L94_RS04945 (K5L94_04945) - 1055792..1056469 (-) 678 WP_274512074.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5L94_RS04950 (K5L94_04950) pilT 1056557..1057594 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  K5L94_RS04955 (K5L94_04955) pilU 1057673..1058836 (+) 1164 WP_049434422.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5L94_RS04960 (K5L94_04960) - 1059038..1060021 (+) 984 WP_274512075.1 aldo/keto reductase -
  K5L94_RS04965 (K5L94_04965) - 1060117..1061016 (-) 900 WP_180879644.1 LysR family transcriptional regulator -
  K5L94_RS04970 (K5L94_04970) - 1061342..1061554 (+) 213 WP_053448999.1 DUF4287 domain-containing protein -
  K5L94_RS04975 (K5L94_04975) - 1061666..1062373 (+) 708 WP_049456208.1 YitT family protein -
  K5L94_RS04980 (K5L94_04980) - 1062447..1062989 (-) 543 WP_053449000.1 DNA-3-methyladenine glycosylase I -
  K5L94_RS04985 (K5L94_04985) - 1063079..1063645 (+) 567 WP_006422622.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 387 a.a.        Molecular weight: 43186.63 Da        Isoelectric Point: 6.6615

>NTDB_id=600664 K5L94_RS04955 WP_049434422.1 1057673..1058836(+) (pilU) [Stenotrophomonas sp. DFS-20110405]
MPHWEPAVNTTATTIDFTSFLKLMAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKT
HECNFAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGY
RNQNSTGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLC
TLHANNANQAMDRIVNFFPEDRRNQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVM
KDSVQLGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1164 bp        

>NTDB_id=600664 K5L94_RS04955 WP_049434422.1 1057673..1058836(+) (pilU) [Stenotrophomonas sp. DFS-20110405]
ATGCCCCATTGGGAGCCCGCCGTGAACACCACCGCGACCACCATCGATTTCACTTCGTTCCTCAAGCTGATGGCGCACCA
GCGCGCCTCGGACCTATTCATCACCGCCGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCCGATCACCCAGA
CCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTGCTCAACGTGATGACCCCGGCGCAGCGCGAGGAATTCGAGAAGACC
CACGAGTGCAACTTCGCCATCGGCCTGTCCGGTGTCGGTCGCTTCCGTGTCAGCTGCTTCTACCAGCGCAACCAGGTCGG
CATGGTGCTGCGCCGCATCGAGACGCGCATTCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAAGACGCTGGCGA
TGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACCTCGCTGGCAGCGATGATCGGTTAC
CGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCAT
CACCCAGCGCGAGGTCGGCATCGATACCGACAGCTGGGAAGCGGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGA
TCATGATCGGCGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTTGCCGAAACCGGCCACCTGGTGCTGTGC
ACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGCAACCAGTTGCT
GATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGATTCCTTCGCCCGATGGCCGCTCGCGCAAGGTGG
CGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGAAGGAAGTGATG
AAGGATTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGA
GGACGCACTGCGCTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGACGCGCGCACCC
TGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-283)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

94.832

0.641
  pilU Acinetobacter baylyi ADP1

63.866

92.248

0.589
  pilU Vibrio cholerae strain A1552

53.371

91.99

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.099

91.731

0.395