Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   K6751_RS05560 Genome accession   NZ_CP082244
Coordinates   1178392..1178805 (-) Length   137 a.a.
NCBI ID   WP_222901096.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain CSMC7.1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1173392..1183805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6751_RS05530 (K6751_05530) - 1173466..1173681 (+) 216 WP_222901091.1 hypothetical protein -
  K6751_RS05535 (K6751_05535) yacG 1173694..1173891 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  K6751_RS05540 (K6751_05540) coaE 1173888..1174499 (-) 612 WP_222901092.1 dephospho-CoA kinase -
  K6751_RS05545 (K6751_05545) pilD 1174496..1175368 (-) 873 WP_222901093.1 prepilin peptidase Machinery gene
  K6751_RS05550 (K6751_05550) pilC 1175369..1176589 (-) 1221 WP_222901094.1 type II secretion system F family protein Machinery gene
  K6751_RS05555 (K6751_05555) pilB 1176593..1178296 (-) 1704 WP_222901095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6751_RS05560 (K6751_05560) pilA 1178392..1178805 (-) 414 WP_222901096.1 pilin Machinery gene
  K6751_RS05565 (K6751_05565) - 1179067..1180668 (+) 1602 WP_222901097.1 PglL family O-oligosaccharyltransferase -
  K6751_RS05575 (K6751_05575) nadC 1180838..1181686 (-) 849 WP_222901098.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 14327.33 Da        Isoelectric Point: 9.2193

>NTDB_id=600502 K6751_RS05560 WP_222901096.1 1178392..1178805(-) (pilA) [Metapseudomonas otitidis strain CSMC7.1]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIARAEANTGLQAIAPLKTAVEDQFSRGVGGASTTVQNLGSGATASPL
GTITATFTSSGTGTLVFVFDRQASPRVKDSRITLTRANDGTWTCTSDVNTDFRPRGC

Nucleotide


Download         Length: 414 bp        

>NTDB_id=600502 K6751_RS05560 WP_222901096.1 1178392..1178805(-) (pilA) [Metapseudomonas otitidis strain CSMC7.1]
ATGAAAGCCCAAAAAGGTTTTACCCTGATCGAACTGATGATCGTCGTTGCGATCATCGGCATTCTGGCCGCCATCGCGAT
TCCGGCCTACCAGAACTACATTGCACGCGCCGAGGCCAACACTGGCCTGCAGGCTATCGCCCCCCTGAAGACTGCCGTTG
AGGATCAGTTCTCCCGTGGCGTTGGCGGCGCCTCCACTACAGTTCAAAACCTTGGCAGCGGCGCTACCGCCAGCCCGCTG
GGTACTATCACTGCAACCTTCACCTCCAGCGGCACCGGCACGCTCGTGTTCGTTTTCGACCGTCAAGCCAGCCCGCGCGT
AAAAGACTCGCGTATTACCCTTACTCGTGCTAACGACGGCACCTGGACCTGCACTTCTGACGTAAACACGGATTTCCGTC
CGCGCGGTTGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-137)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

52.703

100

0.569
  pilA Acinetobacter baumannii strain A118

47.857

100

0.489
  pilA2 Legionella pneumophila str. Paris

41.611

100

0.453
  pilA2 Legionella pneumophila strain ERS1305867

41.611

100

0.453
  pilA Vibrio cholerae strain A1552

41.892

100

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.892

100

0.453
  pilA Vibrio cholerae C6706

41.892

100

0.453
  comP Acinetobacter baylyi ADP1

40

100

0.438
  pilA Ralstonia pseudosolanacearum GMI1000

42.963

98.54

0.423
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.222

98.54

0.416
  pilE Neisseria gonorrhoeae MS11

36.364

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.568

100

0.401
  pilA Vibrio parahaemolyticus RIMD 2210633

44.715

89.781

0.401
  pilA Acinetobacter nosocomialis M2

50.485

75.182

0.38