Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6751_RS05555 Genome accession   NZ_CP082244
Coordinates   1176593..1178296 (-) Length   567 a.a.
NCBI ID   WP_222901095.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain CSMC7.1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1171593..1183296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6751_RS05515 (K6751_05515) - 1171774..1172199 (+) 426 WP_222901090.1 GNAT family N-acetyltransferase -
  K6751_RS05520 (K6751_05520) - 1172196..1172672 (+) 477 WP_052807598.1 hypothetical protein -
  K6751_RS05525 (K6751_05525) - 1172669..1173355 (+) 687 WP_142011982.1 energy-coupling factor ABC transporter permease -
  K6751_RS05530 (K6751_05530) - 1173466..1173681 (+) 216 WP_222901091.1 hypothetical protein -
  K6751_RS05535 (K6751_05535) yacG 1173694..1173891 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  K6751_RS05540 (K6751_05540) coaE 1173888..1174499 (-) 612 WP_222901092.1 dephospho-CoA kinase -
  K6751_RS05545 (K6751_05545) pilD 1174496..1175368 (-) 873 WP_222901093.1 prepilin peptidase Machinery gene
  K6751_RS05550 (K6751_05550) pilC 1175369..1176589 (-) 1221 WP_222901094.1 type II secretion system F family protein Machinery gene
  K6751_RS05555 (K6751_05555) pilB 1176593..1178296 (-) 1704 WP_222901095.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6751_RS05560 (K6751_05560) pilA 1178392..1178805 (-) 414 WP_222901096.1 pilin Machinery gene
  K6751_RS05565 (K6751_05565) - 1179067..1180668 (+) 1602 WP_222901097.1 PglL family O-oligosaccharyltransferase -
  K6751_RS05575 (K6751_05575) nadC 1180838..1181686 (-) 849 WP_222901098.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62508.58 Da        Isoelectric Point: 5.4869

>NTDB_id=600501 K6751_RS05555 WP_222901095.1 1176593..1178296(-) (pilB) [Metapseudomonas otitidis strain CSMC7.1]
MSDSITLTGLARQIVIANLLDEKNAQQAQQQALRNKMSLVTYIVQNKLVKSRPLAEIAAEQFGVAFFDLGAIDKESQPKE
LISEKLIRQHRVLPLWRRGNKLFIGVSDPTNHQAVTDVQFSTGLTTEAILVEDDKLGEAIERFFDSANSGMDDLGDVDLD
GVDIESVDEDKDDKGSGAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGVLHEVARPPIQLASRISAR
LKVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATAVNLIIAQRLARKLCGVCKKEVEVPRDTLLKEGFP
EERIGTFRLYGPVGCDSCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRKDGFSDLRTSGLLKAIQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=600501 K6751_RS05555 WP_222901095.1 1176593..1178296(-) (pilB) [Metapseudomonas otitidis strain CSMC7.1]
ATGAGCGACTCAATTACTCTCACCGGCCTGGCTCGCCAGATTGTGATTGCCAACCTGCTCGACGAGAAGAATGCCCAACA
GGCTCAGCAGCAGGCCCTGCGCAACAAGATGTCGCTGGTAACGTACATCGTCCAGAACAAGCTGGTGAAGAGTCGCCCGC
TCGCGGAAATCGCGGCGGAACAGTTCGGTGTCGCTTTTTTTGACCTGGGCGCCATCGACAAGGAGTCCCAACCCAAAGAG
TTGATCAGCGAGAAGCTGATCCGTCAGCACCGCGTGCTCCCCCTGTGGCGCAGAGGCAACAAGCTCTTTATTGGTGTCTC
GGATCCAACCAACCATCAAGCTGTTACCGATGTGCAGTTCAGTACCGGCCTCACCACCGAAGCCATCCTGGTAGAAGACG
ACAAGCTCGGCGAGGCCATCGAGCGCTTCTTCGACTCGGCGAACAGTGGCATGGACGACCTCGGCGATGTCGACCTCGAT
GGTGTGGATATCGAGAGCGTCGACGAAGACAAGGATGACAAGGGATCGGGGGCCGAGGCCGACGATGCGCCCGTGGTGCG
ATTCGTCAACAAGATGCTGCTGGACGCGATCAAGGGAGGCTCGTCCGACCTGCACTTCGAGCCTTATGAGAAGGCTTACC
GCGTCCGTTTCCGTACCGATGGCGTGCTGCATGAAGTCGCCCGACCGCCGATTCAGTTGGCCAGTCGTATCTCCGCACGC
CTCAAGGTGATGGCGTCCCTGGATATCTCCGAGCGTCGCAAACCGCAGGACGGCCGGATCAAGATGAAGATATCCAAAAC
CAAGGCCATCGACTTTCGTGTCAATACCTTGCCCACGCTGTGGGGCGAGAAGATCGTGATGCGAATCCTGGACCCCTCCA
GCGCCCAGATGGGCATCGACGCACTGGGATATGAGGAATCGCAGAAAGAACTCTACCTCAATGCCTTGAAGCAACCACAA
GGCATGATCCTGGTGACCGGCCCAACCGGATCCGGTAAAACCGTGTCCCTCTACACCGGTCTGAACATCCTCAACACCGT
CGACATCAACATCTCGACAGCCGAAGACCCCGTCGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTTAATCCCCGAC
AGGGCATGGACTTCTCGCAAGCCCTTCGTGCCTTCCTCCGCCAGGACCCCGACGTGATCATGGTGGGTGAGATCCGAGAC
CTGGAGACGGCGGAAATCGCGATCAAGGCCGCACAAACCGGCCACATGGTGATGTCCACCCTCCACACCAACAGCGCTGC
CGAAACACTGACGCGTTTGCGCAACATGGGGGTTCCAGCCTTTAACATTGCCACAGCAGTCAACCTGATCATCGCCCAGC
GCCTTGCACGTAAGCTGTGCGGTGTATGCAAGAAGGAAGTGGAGGTCCCACGCGACACGCTCCTCAAGGAAGGCTTTCCG
GAAGAAAGAATCGGCACCTTCAGACTCTATGGCCCCGTCGGTTGCGATAGCTGCAAAGGCGGCTACAAGGGAAGGGTGGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGATATCGCGGCTC
AGATGCGAAAAGATGGCTTCAGCGACCTGCGCACGTCAGGCCTCTTGAAAGCCATTCAAGGCGTGACGAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.07

100

0.584
  pilB Acinetobacter baylyi ADP1

57.726

99.295

0.573
  pilB Legionella pneumophila strain ERS1305867

55.026

100

0.55
  pilB Vibrio cholerae strain A1552

49.464

98.765

0.489
  pilB Vibrio campbellii strain DS40M4

48.85

99.647

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

50.649

95.062

0.481
  pilF Neisseria gonorrhoeae MS11

47.957

99.295

0.476
  pilF Thermus thermophilus HB27

37.303

100

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.736

93.651

0.372