Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K6982_RS12660 Genome accession   NZ_CP082215
Coordinates   2960533..2961570 (-) Length   345 a.a.
NCBI ID   WP_104604005.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain MI_359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2955533..2966570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6982_RS12640 (K6982_12595) - 2956565..2957104 (+) 540 WP_104605329.1 DNA-3-methyladenine glycosylase I -
  K6982_RS12645 (K6982_12600) - 2957220..2957894 (-) 675 WP_167527011.1 YitT family protein -
  K6982_RS12650 (K6982_12605) - 2958084..2959067 (-) 984 WP_159406471.1 IS5 family transposase -
  K6982_RS12655 (K6982_12610) pilU 2959288..2960418 (-) 1131 WP_104604006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6982_RS12660 (K6982_12615) pilT 2960533..2961570 (-) 1038 WP_104604005.1 type IV pilus twitching motility protein PilT Machinery gene
  K6982_RS12665 (K6982_12620) - 2961814..2962506 (+) 693 WP_159407309.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6982_RS12670 (K6982_12625) proC 2962555..2963406 (+) 852 WP_159407310.1 pyrroline-5-carboxylate reductase -
  K6982_RS12675 (K6982_12630) - 2963869..2964291 (+) 423 WP_104604003.1 HU family DNA-binding protein -
  K6982_RS12680 (K6982_12635) - 2964448..2964630 (-) 183 WP_104604002.1 hypothetical protein -
  K6982_RS12685 (K6982_12640) - 2964665..2965261 (+) 597 WP_274391961.1 M23 family metallopeptidase -
  K6982_RS12690 (K6982_12645) - 2965353..2965655 (+) 303 WP_167526915.1 DUF3861 domain-containing protein -
  K6982_RS12695 (K6982_12650) - 2965880..2966248 (+) 369 WP_104604009.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38625.32 Da        Isoelectric Point: 6.6652

>NTDB_id=600248 K6982_RS12660 WP_104604005.1 2960533..2961570(-) (pilT) [Xanthomonas cucurbitae strain MI_359]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHVSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=600248 K6982_RS12660 WP_104604005.1 2960533..2961570(-) (pilT) [Xanthomonas cucurbitae strain MI_359]
ATGGATATCGCTGAACTATTGGCGTTTTCCGTCAAGAACAAGGCGTCGGACCTGCACCTGTCCGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGTATCAATATTCCGGCGCTGGACCACAAGCAGGTGCATGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAATTCCTCGAGGTCGATTTCTCCTTCGAGATTCCCTCGCTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGAATCGCGGCGCCGGGGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACCCTGGA
AGACCTCGGCTGCCCGCCGATCTTCCGCCAGCTGATCGACCAGCCGCAGGGGCTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGATTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCCATCGAATTCGTGCACGTCTCGCAGAAGTGCCTCATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTTACCGCGGCAGAGACTGGCCACTTGGTGTTCGGCACCTTGCATACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGGGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGGCGCACTGCCGCCTGGGAAATCATGGTCGGTACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTCATCACGCGCAACCAGGCGCGCGAATATGCCAAGGACAAGCGGATATTTGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

79.351

98.261

0.78
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394