Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6982_RS12655 Genome accession   NZ_CP082215
Coordinates   2959288..2960418 (-) Length   376 a.a.
NCBI ID   WP_104604006.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain MI_359     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 2958084..2959067 2959288..2960418 flank 221


Gene organization within MGE regions


Location: 2958084..2960418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6982_RS12650 (K6982_12605) - 2958084..2959067 (-) 984 WP_159406471.1 IS5 family transposase -
  K6982_RS12655 (K6982_12610) pilU 2959288..2960418 (-) 1131 WP_104604006.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41923.21 Da        Isoelectric Point: 6.8287

>NTDB_id=600247 K6982_RS12655 WP_104604006.1 2959288..2960418(-) (pilU) [Xanthomonas cucurbitae strain MI_359]
MSSIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=600247 K6982_RS12655 WP_104604006.1 2959288..2960418(-) (pilU) [Xanthomonas cucurbitae strain MI_359]
ATGAGCAGCATCGACTTCACCTCCTTTCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCGGCGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGTGTGTCGGGC
GTGGGCCGATTCCGTGTGAGCTGCTTCTATCAACGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CACCGTCGAAGAACTGAGCCTGCCGCCGGTCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACT
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGATAG
CTGGGAAAACGCGCTGAAAAACACACTGCGCCAGGCGCCGGACGTCATCATGATCGGCGAGGTGCGTACCCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAGGATCGCCGCAGCCAGTTGTTGATGGACCTGTCGCTCAACCTCAAAGGCGTGGT
CGCCCAGCAGCTGATCCCCACCCCGGATGGGCGCAGCCGCCGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGTGATGGTGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAAGATGCCTTGCGTTATGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGGGATGCCAAGACCCTGGCGCAGGGACTGGACGGGGTGGAGATCGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364