Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5Q02_RS06295 Genome accession   NZ_CP081942
Coordinates   1406027..1407061 (+) Length   344 a.a.
NCBI ID   WP_225837470.1    Uniprot ID   -
Organism   Pseudomonas sp. MM211     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1401027..1412061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Q02_RS06270 (K5Q02_06245) - 1401352..1403334 (-) 1983 WP_225837460.1 dynamin-like GTPase family protein -
  K5Q02_RS06275 (K5Q02_06250) - 1403443..1403739 (-) 297 WP_225837462.1 DUF167 domain-containing protein -
  K5Q02_RS06280 (K5Q02_06255) - 1403744..1404334 (-) 591 WP_225837464.1 YggT family protein -
  K5Q02_RS06285 (K5Q02_06260) proC 1404346..1405164 (-) 819 WP_225837466.1 pyrroline-5-carboxylate reductase -
  K5Q02_RS06290 (K5Q02_06265) - 1405204..1405890 (-) 687 WP_225837468.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5Q02_RS06295 (K5Q02_06270) pilT 1406027..1407061 (+) 1035 WP_225837470.1 type IV pilus twitching motility protein PilT Machinery gene
  K5Q02_RS06300 (K5Q02_06275) pilU 1407170..1408315 (+) 1146 WP_225837471.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5Q02_RS06305 (K5Q02_06280) - 1408281..1409162 (-) 882 WP_225837473.1 TIGR03915 family putative DNA repair protein -
  K5Q02_RS06310 (K5Q02_06285) - 1409162..1410373 (-) 1212 WP_225837475.1 putative DNA modification/repair radical SAM protein -
  K5Q02_RS06315 (K5Q02_06290) - 1410514..1410912 (+) 399 WP_225837477.1 TM2 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37895.57 Da        Isoelectric Point: 6.8890

>NTDB_id=598554 K5Q02_RS06295 WP_225837470.1 1406027..1407061(+) (pilT) [Pseudomonas sp. MM211]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLSMEDLGMGEIFKKVSDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTHGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDACL
KTLVSKGLVSRESAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=598554 K5Q02_RS06295 WP_225837470.1 1406027..1407061(+) (pilT) [Pseudomonas sp. MM211]
ATGGATATCACCGAGCTGCTTGCCTTCAGCGCCAAGCAAGGCGCGTCGGATCTGCACCTCTCTGCAGGTCTGCCACCGAT
GATTCGGGTCGATGGCGACGTGCGTCGGATCAATCTGCCAGCCCTGGATCATAAACAGGTGCACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAATTCCTCGAGACCGACTTCTCGTTCGAAGTGCCGGGTGTAGCGCGC
TTCCGGGTCAACGCCTTCAACCAGAACCGTGGCAGCGGTGCGGTATTCCGTACCATTCCTTCCAAGGTGCTGAGCATGGA
AGACCTCGGCATGGGTGAGATCTTCAAGAAGGTCTCCGACGTGCCGCGCGGGCTGGTGTTGGTAACCGGGCCAACCGGTT
CGGGCAAGTCCACTACCCTGGCGGCGATGCTCGATTACATCAACAGCAACAAGTACCACCACATCCTCACCGTCGAAGAC
CCGATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGATCAACCAGCGTGAGGTGCACCGCGACACCCACGGCTTCTCCGA
AGCCCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCTGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCTCTGCGGCCAAGACCATCGACCGGGTG
GTCGACGTGTTCCCGGCGGAAGAGAAGTCCATGGTGCGTTCGATGCTTTCCGAATCGCTGCAGGCGGTGATTTCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGTGTGGCCGCCCACGAAATCATGATTGGCACCCCAGCGATCCGTAACCTGATCC
GCGAAGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACTGGCGGTGCGCTGGGCATGCAAACCCTGGATGCGTGCCTG
AAAACCCTCGTCTCCAAAGGGCTGGTCAGCCGCGAGAGTGCGCGGGAAAAAGCCAAGACACCTGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Pseudomonas stutzeri DSM 10701

92.733

100

0.927
  pilT Acinetobacter nosocomialis M2

85.756

100

0.858
  pilT Acinetobacter baumannii D1279779

85.465

100

0.855
  pilT Acinetobacter baumannii strain A118

85.465

100

0.855
  pilT Acinetobacter baylyi ADP1

81.977

100

0.82
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39