Detailed information NTG0598555

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K5Q02_RS06300
• Genome accession: NZ_CP081942
• Coordinates: 1407170..1408315 (+)
• Length: 381 a.a.
• NCBI ID: WP_225837471.1
• Uniprot ID: -
• Organism: Pseudomonas sp. MM211
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1402170..1413315

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5Q02_RS06275 (K5Q02_06250)	-	1403443..1403739 (-)	297	WP_225837462.1	DUF167 domain-containing protein	-
K5Q02_RS06280 (K5Q02_06255)	-	1403744..1404334 (-)	591	WP_225837464.1	YggT family protein	-
K5Q02_RS06285 (K5Q02_06260)	proC	1404346..1405164 (-)	819	WP_225837466.1	pyrroline-5-carboxylate reductase	-
K5Q02_RS06290 (K5Q02_06265)	-	1405204..1405890 (-)	687	WP_225837468.1	YggS family pyridoxal phosphate-dependent enzyme	-
K5Q02_RS06295 (K5Q02_06270)	pilT	1406027..1407061 (+)	1035	WP_225837470.1	type IV pilus twitching motility protein PilT	Machinery gene
K5Q02_RS06300 (K5Q02_06275)	pilU	1407170..1408315 (+)	1146	WP_225837471.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K5Q02_RS06305 (K5Q02_06280)	-	1408281..1409162 (-)	882	WP_225837473.1	TIGR03915 family putative DNA repair protein	-
K5Q02_RS06310 (K5Q02_06285)	-	1409162..1410373 (-)	1212	WP_225837475.1	putative DNA modification/repair radical SAM protein	-
K5Q02_RS06315 (K5Q02_06290)	-	1410514..1410912 (+)	399	WP_225837477.1	TM2 domain-containing protein	-
K5Q02_RS06320 (K5Q02_06295)	-	1411109..1412380 (-)	1272	WP_225837479.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42516.77 Da
• Isoelectric Point: 6.6640

>NTDB_id=598555 K5Q02_RS06300 WP_225837471.1 1407170..1408315(+) (pilU) [Pseudomonas sp. MM211]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKTPLTPEMTRETVHSVMTEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGDVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGEHLTTMSQGLSLEVTDENPGQRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=598555 K5Q02_RS06300 WP_225837471.1 1407170..1408315(+) (pilU) [Pseudomonas sp. MM211]
ATGGAATTCGAAAAACTGTTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACCGCTGGCGTGCCGCCATC
GATGAAGGTCAATGGCAAGATCATGCCCGTGACCAAGACGCCGCTAACGCCGGAAATGACCCGCGAGACCGTCCACTCGG
TGATGACCGAGCAGCAACGCCGGGATTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGTGCCCGAGGCGTCGGTCGT
TTCCGCGTCAGTGCCTTTTACCAGCGCAACCTGGCTGGCATGGTGCTGCGCCGTATCGAGACCAACATCCCGACATTGGA
TGACCTCAAACTGCCGGAAATCCTCAAGAAGCTGGCTTTGACCAAGCGCGGTCTGGTGCTGTTCGTCGGCGCCACCGGTA
CGGGTAAGTCCACCTCGCTGGCAGCGATGATCGGCTACCGCAACAAGAACAGCAGTGGTCATATCATCTCCATCGAAGAC
CCGATCGAGTACATTCACCAGCACCAGAACTGCATCGTTACCCAGCGTGAAGTAGGTATCGATACCGATTCCTTCGAAGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATATTGATCGGTGAGGTACGAACTCGGGAAACCATGGATCACG
CCGTGGCCTTCGCCGAGACTGGTCACCTGTGCCTGGCCACTCTGCACGCCAACAACGCCAACCAGGCTCTGGATCGGATC
ATCAACTTCTTCCCGGCGGATCGGCAGAATCAGGTATGGATGGATCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ATTGATTCCAACGCCGGATGGCAAGGGGCGTCGTGCTGTCATCGAGGTGTTGATCAATACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGATGTGCACGAGCTCAAAGCGCTGATGAAACGTTCTACTGAGCAGGGTATGCAAACCTTCGACCAGGCG
CTGTACAACCTCTACAGTCAGGGCGAGATCACCTACGAAGATGCCTTGCTATACGCCGACTCGGCGAATGATCTGCGCCT
GATGATCAAGCTTGGTTCGGAAACCGATGGCGAGCACCTGACCACTATGTCCCAGGGGCTGTCCCTGGAAGTCACGGACG
AGAATCCCGGGCAGCGTTTTCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (110-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	91.864	100	0.919
pilU	Acinetobacter baylyi ADP1	65.826	93.701	0.617
pilU	Vibrio cholerae strain A1552	58.286	91.864	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.155	88.189	0.381