Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5642_RS03430 Genome accession   NZ_CP081860
Coordinates   808242..809288 (+) Length   348 a.a.
NCBI ID   WP_010379026.1    Uniprot ID   A0A2K9JU58
Organism   Pseudoalteromonas piscicida strain WCPW15003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803242..814288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5642_RS03400 (K5642_03400) hemW 803970..805106 (-) 1137 WP_069020912.1 radical SAM family heme chaperone HemW -
  K5642_RS03405 (K5642_03405) - 805103..805696 (-) 594 WP_222242503.1 XTP/dITP diphosphatase -
  K5642_RS03410 (K5642_03410) - 805705..806139 (-) 435 WP_222242505.1 DUF4426 domain-containing protein -
  K5642_RS03415 (K5642_03415) - 806150..806683 (-) 534 WP_010379021.1 YggT family protein -
  K5642_RS03420 (K5642_03420) proC 806693..807514 (-) 822 WP_039494449.1 pyrroline-5-carboxylate reductase -
  K5642_RS03425 (K5642_03425) - 807528..808211 (-) 684 WP_222242507.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5642_RS03430 (K5642_03430) pilT 808242..809288 (+) 1047 WP_010379026.1 type IV pilus twitching motility protein PilT Machinery gene
  K5642_RS03435 (K5642_03435) pilU 809303..810424 (+) 1122 WP_222242509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5642_RS03440 (K5642_03440) - 810402..811289 (+) 888 WP_222242511.1 hypothetical protein -
  K5642_RS03445 (K5642_03445) ruvX 811604..812053 (-) 450 WP_017217006.1 Holliday junction resolvase RuvX -
  K5642_RS03450 (K5642_03450) - 812096..812653 (-) 558 WP_010379034.1 YqgE/AlgH family protein -
  K5642_RS03455 (K5642_03455) gshB 812673..813632 (-) 960 WP_222242513.1 glutathione synthase -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38621.86 Da        Isoelectric Point: 6.3154

>NTDB_id=597813 K5642_RS03430 WP_010379026.1 808242..809288(+) (pilT) [Pseudoalteromonas piscicida strain WCPW15003]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRVNIPPLEAKDVSSLIYDIMNDNQRRDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPNQVLSLDDLGAPDIFTKISDNPRGLVLVTGPTGSGKSTTLAAMVDYINNNKHHHILTIED
PIEFVHDNKQSLINQREVHRDTHSFNAALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLQAVISQTLVKKVGGGRIAAHEIMLGIPAIRNLIREDKIAQMYSAIQTGAMHGMQTMDQCL
TNLLNRGLITQQDARAKAHDKNQFNSGY

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=597813 K5642_RS03430 WP_010379026.1 808242..809288(+) (pilT) [Pseudoalteromonas piscicida strain WCPW15003]
ATGGATATTACAGAATTATTGGCTTTTAGTGTTCAACACAAAGCATCTGACTTACACCTTTCTTCAGGTGTTTCCCCTAT
GATCCGCGTTGACGGGGACGTACGTCGGGTGAATATCCCGCCGTTAGAAGCAAAAGACGTCAGCAGCCTCATCTACGATA
TTATGAATGATAATCAACGTCGTGACTATGAGCAAAACCTTGAGGTAGATTTCTCGTTCGAAGTGCCAAATCTGGCGCGT
TTTCGTGTTAACGCCTTTAATTCAAATCGTGGTCCTGCTGCGGTGTTCCGTACCATTCCAAATCAAGTGCTGAGTTTAGA
TGATTTGGGGGCACCAGACATTTTCACCAAAATTTCCGATAACCCACGTGGCTTAGTATTGGTAACCGGCCCGACAGGTT
CTGGTAAATCAACCACGCTGGCTGCGATGGTGGATTATATTAATAACAACAAACATCACCATATTCTAACCATCGAAGAC
CCGATAGAATTTGTTCACGACAACAAGCAAAGCCTTATTAACCAACGTGAAGTACATAGAGACACGCACAGCTTTAACGC
GGCGCTGAGAAGTGCACTCCGTGAAGATCCTGACGTTATTTTAGTTGGTGAATTACGTGATCTTGAGACCATTCGTTTAG
CGCTAACTGCCGCGGAAACAGGCCACTTAGTATTTGGTACTTTGCATACTACCTCAGCACCTAAAACCATCGACCGTATT
GTGGACGTGTTCCCAGGTGAAGAAAAAGACATGGTGCGTTCTATGCTGTCAGAGTCGCTACAAGCGGTGATTTCACAAAC
TTTGGTGAAAAAAGTTGGCGGCGGGCGAATCGCAGCGCATGAAATCATGCTCGGGATCCCCGCTATTCGTAACCTTATTC
GTGAAGATAAGATTGCACAGATGTATTCGGCAATTCAAACCGGTGCAATGCACGGTATGCAGACCATGGATCAATGTCTT
ACAAATTTATTAAACCGTGGTTTGATCACACAGCAAGATGCGCGTGCAAAAGCGCACGATAAAAATCAGTTTAATAGCGG
CTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9JU58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.035

98.851

0.761
  pilT Acinetobacter baumannii D1279779

77.035

98.851

0.761
  pilT Acinetobacter baumannii strain A118

77.035

98.851

0.761
  pilT Acinetobacter baylyi ADP1

76.744

98.851

0.759
  pilT Pseudomonas stutzeri DSM 10701

75.872

98.851

0.75
  pilT Pseudomonas aeruginosa PAK

75.581

98.851

0.747
  pilT Legionella pneumophila strain Lp02

71.221

98.851

0.704
  pilT Legionella pneumophila strain ERS1305867

71.221

98.851

0.704
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.217

96.839

0.69
  pilT Vibrio cholerae strain A1552

71.217

96.839

0.69
  pilT Neisseria meningitidis 8013

69.501

97.989

0.681
  pilT Neisseria gonorrhoeae MS11

69.208

97.989

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

99.138

0.526
  pilU Vibrio cholerae strain A1552

40.233

98.563

0.397
  pilU Pseudomonas stutzeri DSM 10701

39.942

98.563

0.394
  pilU Acinetobacter baylyi ADP1

38.953

98.851

0.385