Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5642_RS03435 Genome accession   NZ_CP081860
Coordinates   809303..810424 (+) Length   373 a.a.
NCBI ID   WP_222242509.1    Uniprot ID   -
Organism   Pseudoalteromonas piscicida strain WCPW15003     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 804303..815424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5642_RS03405 (K5642_03405) - 805103..805696 (-) 594 WP_222242503.1 XTP/dITP diphosphatase -
  K5642_RS03410 (K5642_03410) - 805705..806139 (-) 435 WP_222242505.1 DUF4426 domain-containing protein -
  K5642_RS03415 (K5642_03415) - 806150..806683 (-) 534 WP_010379021.1 YggT family protein -
  K5642_RS03420 (K5642_03420) proC 806693..807514 (-) 822 WP_039494449.1 pyrroline-5-carboxylate reductase -
  K5642_RS03425 (K5642_03425) - 807528..808211 (-) 684 WP_222242507.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5642_RS03430 (K5642_03430) pilT 808242..809288 (+) 1047 WP_010379026.1 type IV pilus twitching motility protein PilT Machinery gene
  K5642_RS03435 (K5642_03435) pilU 809303..810424 (+) 1122 WP_222242509.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5642_RS03440 (K5642_03440) - 810402..811289 (+) 888 WP_222242511.1 hypothetical protein -
  K5642_RS03445 (K5642_03445) ruvX 811604..812053 (-) 450 WP_017217006.1 Holliday junction resolvase RuvX -
  K5642_RS03450 (K5642_03450) - 812096..812653 (-) 558 WP_010379034.1 YqgE/AlgH family protein -
  K5642_RS03455 (K5642_03455) gshB 812673..813632 (-) 960 WP_222242513.1 glutathione synthase -
  K5642_RS03460 (K5642_03460) rsmE 813690..814424 (-) 735 WP_010379040.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  K5642_RS03465 (K5642_03465) - 814487..814984 (-) 498 WP_039494456.1 hypothetical protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41104.96 Da        Isoelectric Point: 6.0556

>NTDB_id=597814 K5642_RS03435 WP_222242509.1 809303..810424(+) (pilU) [Pseudoalteromonas piscicida strain WCPW15003]
MNIEPFLQAMADQQASDLFVSAGLAVSAKIDGELRALSEESLNAEQSLNLVTSIMSDKQKQEFFNTKECNFAIANDIGRF
RVSAFWQRDCAGMVIRRIVTAIPDVNELGLPSVLTDVIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNQRGHILTIEDP
IEFVHQHQKSIITQREVGIDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDRIM
HLVPKEKHDKLKYDLALNLRAIVAQQLVPSSKGEGREAAIEVLLNSPLVAELIKKGDIGSIKEAMAKSKDMGMQTFDQAL
FELYKQQRINYADALHHADSPNDLRLMIKLQNNEQKGAGFLQGVTVDGLDSKN

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=597814 K5642_RS03435 WP_222242509.1 809303..810424(+) (pilU) [Pseudoalteromonas piscicida strain WCPW15003]
ATGAATATAGAACCGTTTTTGCAAGCGATGGCCGATCAGCAAGCATCCGACTTATTCGTTTCGGCAGGACTTGCGGTGAG
CGCCAAAATTGATGGCGAACTTAGAGCGTTAAGTGAAGAAAGCTTAAATGCCGAGCAATCACTGAATCTTGTTACCTCGA
TTATGAGCGACAAGCAAAAGCAAGAATTCTTTAACACTAAAGAGTGTAATTTTGCGATTGCCAATGATATCGGGCGTTTT
CGTGTTTCAGCATTTTGGCAACGAGACTGTGCGGGTATGGTTATTCGCCGTATCGTTACCGCGATTCCCGATGTCAATGA
GCTAGGTTTGCCGTCGGTACTGACCGATGTGATCATGTCCAAACGTGGCTTGGTGTTATTTGTAGGTGGTACGGGGACGG
GTAAGTCAACCTCGCTTGCTGCACTGCTCGGTTATCGCAATCGTAACCAACGTGGCCATATTTTGACCATTGAAGATCCC
ATCGAATTTGTGCATCAACACCAAAAAAGCATCATTACTCAGCGTGAAGTGGGCATTGATACTGACAGTTTTGAATCTGC
CTTAAAAAGTTCATTACGCCAAGCGCCAGATGTTATTTTAATTGGTGAAATTCGCTCGCAAGAAACCATGGAGTATGCAC
TGAGCTTTGCTGAAACGGGGCATTTATGCGTTGCAACCTTACACGCCAATAATGCTAACCAAGCCATCGACCGTATCATG
CACTTAGTCCCCAAAGAAAAGCACGATAAACTGAAGTACGATTTGGCGCTGAACTTGCGCGCTATTGTGGCGCAACAACT
AGTGCCTTCTTCAAAAGGCGAAGGTCGAGAGGCGGCCATTGAAGTGTTACTGAACTCCCCACTCGTTGCCGAGCTTATTA
AAAAAGGCGATATTGGCTCGATTAAAGAGGCAATGGCAAAATCGAAAGACATGGGTATGCAAACCTTTGACCAAGCGTTG
TTTGAGCTTTATAAGCAGCAGCGGATCAACTATGCCGACGCACTACATCATGCTGACTCGCCAAATGATCTGCGCTTGAT
GATCAAGCTGCAAAATAATGAACAAAAAGGTGCGGGCTTCCTACAAGGGGTTACGGTAGATGGGTTGGATTCGAAGAACT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

56.499

100

0.571
  pilU Pseudomonas stutzeri DSM 10701

60.399

94.102

0.568
  pilU Vibrio cholerae strain A1552

56.131

98.391

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.778

96.515

0.413
  pilT Legionella pneumophila strain ERS1305867

41.742

89.276

0.373
  pilT Legionella pneumophila strain Lp02

41.742

89.276

0.373
  pilT Pseudomonas aeruginosa PAK

40.29

92.493

0.373
  pilT Acinetobacter baylyi ADP1

40.643

91.689

0.373
  pilT Acinetobacter nosocomialis M2

40.351

91.689

0.37
  pilT Acinetobacter baumannii D1279779

40.351

91.689

0.37
  pilT Acinetobacter baumannii strain A118

40.351

91.689

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.13

92.493

0.362