Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K5K93_RS07800 Genome accession   NZ_CP081826
Coordinates   1686458..1687495 (-) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas sp. DR822     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1681458..1692495
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K93_RS07775 (K5K93_07775) - 1681774..1682673 (+) 900 WP_033830519.1 LysR family transcriptional regulator -
  K5K93_RS07780 (K5K93_07780) - 1682731..1683714 (-) 984 WP_222390450.1 aldo/keto reductase -
  K5K93_RS07785 (K5K93_07785) - 1683803..1684384 (-) 582 WP_222390452.1 hypothetical protein -
  K5K93_RS07790 (K5K93_07790) - 1684492..1685145 (-) 654 WP_222390454.1 PIN domain-containing protein -
  K5K93_RS07795 (K5K93_07795) pilU 1685215..1686309 (-) 1095 WP_229291357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5K93_RS07800 (K5K93_07800) pilT 1686458..1687495 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  K5K93_RS07805 (K5K93_07805) - 1687583..1688260 (+) 678 WP_222390456.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5K93_RS07810 (K5K93_07810) proC 1688277..1689098 (+) 822 WP_222390459.1 pyrroline-5-carboxylate reductase -
  K5K93_RS07815 (K5K93_07815) - 1689153..1690028 (-) 876 WP_222390461.1 LysR family transcriptional regulator -
  K5K93_RS07820 (K5K93_07820) - 1690127..1690729 (+) 603 WP_222390464.1 short chain dehydrogenase -
  K5K93_RS07825 (K5K93_07825) soxR 1690767..1691201 (-) 435 WP_222390467.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=597590 K5K93_RS07800 WP_004147099.1 1686458..1687495(-) (pilT) [Stenotrophomonas sp. DR822]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=597590 K5K93_RS07800 WP_004147099.1 1686458..1687495(-) (pilT) [Stenotrophomonas sp. DR822]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCATCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTTCGCCGGATCAACATTCCAGCCCTGGACCACAAGCAGGTCCACGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAGTTCCTCGAAGTCGATTTCTCCTTCGAGATCCCGTCGCTGGCGCGC
TTCCGTGTCAATGCGTTCAACCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGCCTGCCCGCCGCTGTTCCGCGAGGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAGTACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAATGA
AGCGCTGCGTTCGGCACTGCGTGAGGACCCGGACATCATCCTGGTCGGCGAACTGCGTGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTCGGTGGCGGTCGTACCGCCGCCTGGGAAATCATGGTCGGCACCCCAGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAATACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391