Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K5K93_RS07795 Genome accession   NZ_CP081826
Coordinates   1685215..1686309 (-) Length   364 a.a.
NCBI ID   WP_229291357.1    Uniprot ID   -
Organism   Stenotrophomonas sp. DR822     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680215..1691309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K93_RS07770 (K5K93_07770) - 1681177..1681389 (-) 213 WP_222390449.1 DUF4287 domain-containing protein -
  K5K93_RS07775 (K5K93_07775) - 1681774..1682673 (+) 900 WP_033830519.1 LysR family transcriptional regulator -
  K5K93_RS07780 (K5K93_07780) - 1682731..1683714 (-) 984 WP_222390450.1 aldo/keto reductase -
  K5K93_RS07785 (K5K93_07785) - 1683803..1684384 (-) 582 WP_222390452.1 hypothetical protein -
  K5K93_RS07790 (K5K93_07790) - 1684492..1685145 (-) 654 WP_222390454.1 PIN domain-containing protein -
  K5K93_RS07795 (K5K93_07795) pilU 1685215..1686309 (-) 1095 WP_229291357.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K5K93_RS07800 (K5K93_07800) pilT 1686458..1687495 (-) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  K5K93_RS07805 (K5K93_07805) - 1687583..1688260 (+) 678 WP_222390456.1 YggS family pyridoxal phosphate-dependent enzyme -
  K5K93_RS07810 (K5K93_07810) proC 1688277..1689098 (+) 822 WP_222390459.1 pyrroline-5-carboxylate reductase -
  K5K93_RS07815 (K5K93_07815) - 1689153..1690028 (-) 876 WP_222390461.1 LysR family transcriptional regulator -
  K5K93_RS07820 (K5K93_07820) - 1690127..1690729 (+) 603 WP_222390464.1 short chain dehydrogenase -
  K5K93_RS07825 (K5K93_07825) soxR 1690767..1691201 (-) 435 WP_222390467.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40579.63 Da        Isoelectric Point: 6.7682

>NTDB_id=597589 K5K93_RS07795 WP_229291357.1 1685215..1686309(-) (pilU) [Stenotrophomonas sp. DR822]
MAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLIPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=597589 K5K93_RS07795 WP_229291357.1 1685215..1686309(-) (pilU) [Stenotrophomonas sp. DR822]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCCGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATTTCGCC
AATCACCCAGACGCCACTGACGCCGCAGCAGAGCCGCGACCTGGTGCTGAACGTGATGACGCCGGCGCAGCGCGAAGAAT
TCGAAAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGCGTCGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATCCCGACCGTGGAAGAGCTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCTACCGGTACCGGTAAATCAACCTCGCTGGCAGCGA
TGATCGGTTACCGCAACCAGAATTCGACCGGGCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGATGTGATCATGATCGGCGAAGTGCGTACCCGCGAGGGCATGGACCATGCCATCGCCTTCGCCGAAACCGGCCATC
TGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGATCGCCGC
AACCAGCTGTTGATGGATCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGTTGATTCCTTCGCCGGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAGCTGATGAAGGAATCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAACTGTACCAGGCCGGTGAG
ATCAGCTACGAGGACGCACTGCGCTACGCCGATTCGCAGAACGAGGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
TGCCCGCACCCTGTCGCAGGGGCTGGATGGCGTGGAGATTTCAGAGGTCCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.436

98.352

0.673
  pilU Acinetobacter baylyi ADP1

64.655

95.604

0.618
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.609

94.78

0.404
  pilT Pseudomonas aeruginosa PAK

41.231

89.286

0.368
  pilT Acinetobacter baumannii strain A118

40.606

90.659

0.368
  pilT Acinetobacter nosocomialis M2

40.606

90.659

0.368
  pilT Acinetobacter baumannii D1279779

40.606

90.659

0.368
  pilT Legionella pneumophila strain ERS1305867

40.867

88.736

0.363
  pilT Legionella pneumophila strain Lp02

40.867

88.736

0.363