Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K4H28_RS10705 Genome accession   NZ_CP081150
Coordinates   2293163..2294206 (-) Length   347 a.a.
NCBI ID   WP_221005189.1    Uniprot ID   -
Organism   Deefgea tanakiae strain D25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2288163..2299206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H28_RS10695 (K4H28_10695) - 2289788..2291887 (-) 2100 WP_221005188.1 FimV family protein -
  K4H28_RS10700 (K4H28_10700) pilU 2292015..2293151 (-) 1137 WP_308443444.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K4H28_RS10705 (K4H28_10705) pilT 2293163..2294206 (-) 1044 WP_221005189.1 type IV pilus twitching motility protein PilT Machinery gene
  K4H28_RS10710 (K4H28_10710) - 2294296..2295006 (+) 711 WP_221005190.1 YggS family pyridoxal phosphate-dependent enzyme -
  K4H28_RS10715 (K4H28_10715) proC 2295003..2295818 (+) 816 WP_221005191.1 pyrroline-5-carboxylate reductase -
  K4H28_RS10720 (K4H28_10720) - 2295834..2296421 (+) 588 WP_255573504.1 YggT family protein -
  K4H28_RS10725 (K4H28_10725) - 2296421..2296714 (+) 294 WP_221005192.1 DUF167 family protein -
  K4H28_RS10730 (K4H28_10730) - 2296733..2297146 (-) 414 WP_221005193.1 FxsA family protein -
  K4H28_RS10735 (K4H28_10735) cutA 2297145..2297483 (+) 339 WP_221005194.1 divalent-cation tolerance protein CutA -
  K4H28_RS10740 (K4H28_10740) dsbD 2297480..2299033 (+) 1554 WP_221005195.1 protein-disulfide reductase DsbD -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38353.07 Da        Isoelectric Point: 6.8272

>NTDB_id=595391 K4H28_RS10705 WP_221005189.1 2293163..2294206(-) (pilT) [Deefgea tanakiae strain D25]
MEISELLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINLPAMEHKDVHDMVYDIMNDGQRKIYEDTLECDFSFEIPNLAR
FRVNAFVQNRGAGAVFRTIPSKVLSLEELGCPKVFQDIAQNPRGLVLVTGPTGSGKSTTLAAMVNFINDNDYGHILTVED
PIEFVHQSKKCLVNQREVGPHTMSFSNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGQGRVAAHEIMIGIPAIRNLIRENKIAQMYSSIQTGSQYGMQTLDQC
LQNLVQRNIVSIADARSKAAIPDNFKG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=595391 K4H28_RS10705 WP_221005189.1 2293163..2294206(-) (pilT) [Deefgea tanakiae strain D25]
ATGGAAATCTCAGAACTCTTAGCCTTTTCGGTAAAGAATAAAGCATCCGACTTACATCTTTCCGCAGGCTTGCCGCCAAT
GATTCGCGTACACGGTGATGTTCGCCGTATTAATTTGCCTGCGATGGAGCATAAAGACGTGCACGATATGGTGTACGACA
TTATGAATGATGGCCAGCGTAAAATTTACGAAGATACATTAGAGTGCGATTTTTCATTTGAAATTCCGAATCTGGCGCGT
TTCCGCGTCAATGCCTTTGTACAAAATCGTGGCGCAGGCGCAGTATTTCGGACCATTCCATCTAAGGTCTTGTCACTGGA
AGAGCTGGGTTGCCCAAAAGTATTTCAAGATATCGCGCAAAATCCACGAGGTTTAGTTCTGGTTACTGGCCCAACTGGCT
CAGGTAAGTCGACCACATTGGCCGCGATGGTCAACTTTATTAATGATAATGACTACGGCCATATTTTGACCGTTGAAGAC
CCGATTGAGTTTGTACATCAATCAAAAAAATGTTTAGTGAATCAACGTGAAGTTGGCCCCCATACGATGTCGTTCTCCAA
TGCGCTGCGTAGTGCTTTGCGTGAAGATCCTGATGTGATCTTGGTGGGTGAAATGCGTGATTTGGAAACCATTCGTTTAG
CTCTCACTGCTGCTGAAACGGGGCATTTAGTGTTTGGTACCTTGCATACGACCTCTGCCGCGAAAACGATTGACCGCGTG
GTTGACGTGTTCCCTGCCGCTGAGAAAGAAATGGTTCGTTCGATGTTGTCTGAATCATTACGTGCGGTTATTTCACAAAC
CTTGTTGAAGACAAAAGACGGGCAAGGGCGTGTTGCGGCACATGAAATTATGATTGGTATTCCTGCGATTCGAAATTTGA
TTCGTGAAAATAAGATTGCCCAAATGTATTCGTCCATTCAAACTGGATCGCAATATGGGATGCAGACTTTGGATCAATGT
TTACAAAACTTAGTACAAAGAAACATTGTTTCAATTGCGGATGCGCGCAGTAAAGCAGCAATACCTGATAATTTTAAAGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.261

99.424

0.778
  pilT Acinetobacter baumannii D1279779

78.261

99.424

0.778
  pilT Acinetobacter baumannii strain A118

78.261

99.424

0.778
  pilT Pseudomonas aeruginosa PAK

77.101

99.424

0.767
  pilT Pseudomonas stutzeri DSM 10701

77.101

99.424

0.767
  pilT Acinetobacter baylyi ADP1

76.232

99.424

0.758
  pilT Legionella pneumophila strain Lp02

73.451

97.695

0.718
  pilT Legionella pneumophila strain ERS1305867

73.451

97.695

0.718
  pilT Neisseria meningitidis 8013

72.861

97.695

0.712
  pilT Neisseria gonorrhoeae MS11

72.566

97.695

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.406

0.657
  pilT Vibrio cholerae strain A1552

67.456

97.406

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

39.481

100

0.395