Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K4H28_RS10700 Genome accession   NZ_CP081150
Coordinates   2292015..2293151 (-) Length   378 a.a.
NCBI ID   WP_308443444.1    Uniprot ID   -
Organism   Deefgea tanakiae strain D25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2287015..2298151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4H28_RS10690 (K4H28_10690) - 2287600..2289483 (+) 1884 WP_221005187.1 methyl-accepting chemotaxis protein -
  K4H28_RS10695 (K4H28_10695) - 2289788..2291887 (-) 2100 WP_221005188.1 FimV family protein -
  K4H28_RS10700 (K4H28_10700) pilU 2292015..2293151 (-) 1137 WP_308443444.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K4H28_RS10705 (K4H28_10705) pilT 2293163..2294206 (-) 1044 WP_221005189.1 type IV pilus twitching motility protein PilT Machinery gene
  K4H28_RS10710 (K4H28_10710) - 2294296..2295006 (+) 711 WP_221005190.1 YggS family pyridoxal phosphate-dependent enzyme -
  K4H28_RS10715 (K4H28_10715) proC 2295003..2295818 (+) 816 WP_221005191.1 pyrroline-5-carboxylate reductase -
  K4H28_RS10720 (K4H28_10720) - 2295834..2296421 (+) 588 WP_255573504.1 YggT family protein -
  K4H28_RS10725 (K4H28_10725) - 2296421..2296714 (+) 294 WP_221005192.1 DUF167 family protein -
  K4H28_RS10730 (K4H28_10730) - 2296733..2297146 (-) 414 WP_221005193.1 FxsA family protein -
  K4H28_RS10735 (K4H28_10735) cutA 2297145..2297483 (+) 339 WP_221005194.1 divalent-cation tolerance protein CutA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42616.84 Da        Isoelectric Point: 6.3751

>NTDB_id=595390 K4H28_RS10700 WP_308443444.1 2292015..2293151(-) (pilU) [Deefgea tanakiae strain D25]
MEKEQAAKFMHDLLRHMRGKNASDLFITVDFPPAMKIDGRVTPVSNQQLTAQHTKELARAIMNDRQAEDFEANKECNFAI
SPGTMGRFRVNAFMQQGRVGMVLRTINSEIPKLEELNLPPVLRDIAMTKRGLVIFVGGTGSGKSTSLAAMIGHRNEYAYD
HIITIEDPIEYVHEHKNSIITQREVGVDTDSWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSS
NQALDRIINFFPEERRSQLLMDLSLNLKAFVSQRLVPHLSGKGRVAAVEVMLNSPLISELIFKGEVHEIKEIMKKSRELG
MQTFDQSLFDLFEANRISYEDALRNADSVNDLRLQIKLNGTESKNRDVLSGLDHLDIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=595390 K4H28_RS10700 WP_308443444.1 2292015..2293151(-) (pilU) [Deefgea tanakiae strain D25]
ATGGAAAAAGAACAAGCCGCTAAGTTTATGCATGATTTGTTGCGCCATATGCGTGGCAAAAATGCTTCGGATCTTTTTAT
TACGGTCGATTTTCCACCAGCCATGAAGATTGACGGTCGAGTAACGCCGGTTTCAAATCAGCAATTGACCGCGCAGCACA
CCAAAGAATTAGCGCGTGCGATTATGAATGATCGCCAGGCTGAGGACTTTGAAGCCAACAAAGAATGTAACTTTGCAATT
AGCCCAGGCACGATGGGGCGTTTTCGCGTAAATGCTTTTATGCAGCAAGGTCGCGTCGGAATGGTATTGCGTACGATTAA
CTCCGAAATTCCTAAGCTTGAAGAACTCAACCTGCCGCCTGTGCTACGTGATATCGCCATGACTAAGCGTGGCTTAGTTA
TTTTTGTGGGGGGGACTGGATCAGGTAAATCGACCTCTTTAGCGGCCATGATCGGTCATCGTAATGAATATGCATACGAT
CACATCATTACGATTGAAGACCCCATTGAATATGTGCATGAGCACAAGAACTCAATCATTACTCAGCGTGAGGTTGGCGT
TGATACTGATTCTTGGATGGCAGCACTTAAAAACACCTTGCGCCAAGCGCCGGATGTGATTTTGATTGGTGAGATTCGTG
ATCGTGAAACGATGGATTATGCCATTGCCTTTGCTGAAACGGGGCATTTGTGCATGGCCACCTTGCATGCCAACTCGTCG
AATCAGGCTCTAGATCGGATTATTAACTTTTTCCCTGAAGAGCGACGTTCGCAGCTCTTGATGGATTTGTCGTTGAATTT
GAAGGCCTTTGTTTCTCAACGATTGGTACCACACCTATCAGGAAAAGGTCGTGTTGCCGCTGTAGAAGTGATGCTCAATT
CACCGTTGATTTCAGAGTTGATTTTCAAAGGAGAAGTACACGAGATTAAAGAAATCATGAAAAAATCTCGTGAGCTTGGC
ATGCAAACGTTTGATCAATCTTTATTTGATTTGTTTGAGGCCAATCGAATTAGCTATGAAGATGCTCTGCGTAATGCTGA
TTCAGTCAATGACTTGCGTCTACAAATTAAGCTTAACGGAACAGAGTCCAAAAATAGAGATGTATTGTCTGGGCTAGATC
ATCTCGATATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.732

94.709

0.585
  pilU Acinetobacter baylyi ADP1

57.65

96.825

0.558
  pilU Vibrio cholerae strain A1552

54

92.593

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.97

88.624

0.407
  pilT Acinetobacter baylyi ADP1

43.731

86.508

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.09

88.624

0.373
  pilT Legionella pneumophila strain Lp02

42.598

87.566

0.373
  pilT Legionella pneumophila strain ERS1305867

42.598

87.566

0.373
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37
  pilT Acinetobacter nosocomialis M2

42.991

84.921

0.365
  pilT Acinetobacter baumannii D1279779

42.991

84.921

0.365
  pilT Acinetobacter baumannii strain A118

42.991

84.921

0.365