Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K2O51_RS27345 Genome accession   NZ_CP080767
Coordinates   3162555..3163598 (-) Length   347 a.a.
NCBI ID   WP_011298946.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3157555..3168598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS27315 (K2O51_27315) - 3158513..3158905 (+) 393 WP_011298940.1 CidA/LrgA family protein -
  K2O51_RS27320 (K2O51_27320) - 3158902..3159627 (+) 726 WP_011298941.1 LrgB family protein -
  K2O51_RS27325 (K2O51_27325) - 3159714..3160085 (+) 372 WP_011298942.1 VOC family protein -
  K2O51_RS27330 (K2O51_27330) - 3160108..3160797 (+) 690 WP_011298943.1 YafY family protein -
  K2O51_RS27335 (K2O51_27335) - 3160804..3161298 (-) 495 WP_011298944.1 glutathione peroxidase -
  K2O51_RS27340 (K2O51_27340) pilU 3161330..3162469 (-) 1140 WP_011298945.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K2O51_RS27345 (K2O51_27345) pilT 3162555..3163598 (-) 1044 WP_011298946.1 type IV pilus twitching motility protein PilT Machinery gene
  K2O51_RS27350 (K2O51_27350) - 3163677..3164366 (+) 690 WP_140951563.1 YggS family pyridoxal phosphate-dependent enzyme -
  K2O51_RS27355 (K2O51_27355) proC 3164412..3165248 (+) 837 WP_011298948.1 pyrroline-5-carboxylate reductase -
  K2O51_RS27360 (K2O51_27360) ubiA 3165330..3166187 (-) 858 WP_011298949.1 4-hydroxybenzoate octaprenyltransferase -
  K2O51_RS27365 (K2O51_27365) - 3166338..3166823 (-) 486 WP_011298950.1 Dps family protein -
  K2O51_RS27370 (K2O51_27370) - 3167091..3168041 (-) 951 WP_011298951.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38392.20 Da        Isoelectric Point: 6.9772

>NTDB_id=594840 K2O51_RS27345 WP_011298946.1 3162555..3163598(-) (pilT) [Cupriavidus pinatubonensis strain HN-2]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVASMAHKDVHAMVYDIMSDSQRKVYEERLEIDFSFEIGGLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEELRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNENDMGHILTVED
PIEFVHQSKKSLINQRELGPHTHSFANALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRSLISYSDARAIAKNPDAFMG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=594840 K2O51_RS27345 WP_011298946.1 3162555..3163598(-) (pilT) [Cupriavidus pinatubonensis strain HN-2]
ATGGACATCGCGCAGCTATTGGCTTTCGCAGTCAAGAACAAGGCGTCCGATTTGCATCTCTCGGCGGACATGCCGCCGAT
GGTACGTATCCACGGCGACATGCGGCGCATCAATGTCGCGTCCATGGCGCACAAGGACGTCCACGCCATGGTGTACGACA
TCATGAGCGACTCCCAGCGCAAGGTTTACGAAGAACGGCTCGAGATCGATTTCTCGTTTGAGATCGGCGGCCTGTCGCGT
TTCCGGGTCAACGCCTACAACACGCAACGCGGCGCGGCCGCGGTGTTCCGTACGATTCCCTCGAAGGTTCTCACGCTTGA
AGAGCTGCGCGCGCCGGCGGTGTTCGCCGACCTGTGCATGAAGCCGCGTGGCCTGGTGCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTCGCGGCAATGGTCGATCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAAGAC
CCGATCGAATTCGTGCACCAGTCGAAGAAGAGCCTGATCAACCAGCGCGAACTCGGTCCGCACACGCATTCGTTTGCCAA
CGCGCTGCGTTCGGCACTGCGTGAAGACCCGGACGTGATCCTCGTCGGCGAATTGCGCGATCTTGAAACCATCCGCCTCG
CGCTGACCGCGGCGGAAACCGGCCACCTGGTTTTCGGCACGCTGCACACGAGTTCCGCGGCCAAGACCATCGACCGTGTG
GTAGACGTGTTCCCGCCCGAAGAGAAGGACATGGTGCGCACAATGCTGTCCGAATCGCTCGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGACGGCAATGGCCGTACCGCGGCGCACGAGATCATGATCGCCACGCCCGCGATCCGCCATCTGA
TCCGCGAGAACAAGATTGCACAGATGTACTCGATGATGCAGACCAGCAGCGGCCTGGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCAGCCTGATCAGCTACAGCGACGCGCGCGCCATCGCCAAGAACCCGGACGCCTTCATGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.965

99.712

0.718
  pilT Acinetobacter baumannii D1279779

71.965

99.712

0.718
  pilT Acinetobacter baumannii strain A118

71.965

99.712

0.718
  pilT Pseudomonas stutzeri DSM 10701

70.145

99.424

0.697
  pilT Pseudomonas aeruginosa PAK

70.145

99.424

0.697
  pilT Acinetobacter baylyi ADP1

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Acinetobacter baylyi ADP1

40.116

99.135

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Vibrio cholerae strain A1552

40.597

96.542

0.392