Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K2O51_RS27340 Genome accession   NZ_CP080767
Coordinates   3161330..3162469 (-) Length   379 a.a.
NCBI ID   WP_011298945.1    Uniprot ID   -
Organism   Cupriavidus pinatubonensis strain HN-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156330..3167469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2O51_RS27310 (K2O51_27310) glcF 3157184..3158437 (+) 1254 WP_011298939.1 glycolate oxidase subunit GlcF -
  K2O51_RS27315 (K2O51_27315) - 3158513..3158905 (+) 393 WP_011298940.1 CidA/LrgA family protein -
  K2O51_RS27320 (K2O51_27320) - 3158902..3159627 (+) 726 WP_011298941.1 LrgB family protein -
  K2O51_RS27325 (K2O51_27325) - 3159714..3160085 (+) 372 WP_011298942.1 VOC family protein -
  K2O51_RS27330 (K2O51_27330) - 3160108..3160797 (+) 690 WP_011298943.1 YafY family protein -
  K2O51_RS27335 (K2O51_27335) - 3160804..3161298 (-) 495 WP_011298944.1 glutathione peroxidase -
  K2O51_RS27340 (K2O51_27340) pilU 3161330..3162469 (-) 1140 WP_011298945.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K2O51_RS27345 (K2O51_27345) pilT 3162555..3163598 (-) 1044 WP_011298946.1 type IV pilus twitching motility protein PilT Machinery gene
  K2O51_RS27350 (K2O51_27350) - 3163677..3164366 (+) 690 WP_140951563.1 YggS family pyridoxal phosphate-dependent enzyme -
  K2O51_RS27355 (K2O51_27355) proC 3164412..3165248 (+) 837 WP_011298948.1 pyrroline-5-carboxylate reductase -
  K2O51_RS27360 (K2O51_27360) ubiA 3165330..3166187 (-) 858 WP_011298949.1 4-hydroxybenzoate octaprenyltransferase -
  K2O51_RS27365 (K2O51_27365) - 3166338..3166823 (-) 486 WP_011298950.1 Dps family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42187.43 Da        Isoelectric Point: 6.8261

>NTDB_id=594839 K2O51_RS27340 WP_011298945.1 3161330..3162469(-) (pilU) [Cupriavidus pinatubonensis strain HN-2]
MLDRESAAKYINDLLELMVSNRGSDLFITSDFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQVKEFDDSRECNFA
ISAPKAGRFRVSAFIQQGKAGMVVRTINTRIPSVSDLDLPPTLHEIVMSKRGLVIVTGATGSGKSTTLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCVVTQREVGIDTESWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRIVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRAGRKGRVPAVEIMIGTPLVADLIFKGEIHELKEVIKKSREQ
GMISFDQALFDLHESGKITYEDALRNADSLNDLRLMIKLHSATRKDSDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=594839 K2O51_RS27340 WP_011298945.1 3161330..3162469(-) (pilU) [Cupriavidus pinatubonensis strain HN-2]
ATGCTCGACCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTTATGGTGAGCAACCGCGGGTCGGACCTGTT
CATCACCTCCGACTTTCCGCCGGCTATCAAGGTCGATGGCAAGATCACGCCCGTCTCGCAGCAGCCGCTGAATCCTACGC
AGGCGCTGGGCCTGGTGCGTTCGATCATGAACGAGCGCCAGGTCAAGGAGTTCGACGACAGCCGTGAATGCAACTTCGCC
ATCTCGGCGCCCAAGGCCGGCCGCTTCCGCGTATCGGCCTTTATCCAGCAGGGCAAGGCCGGCATGGTGGTGCGGACCAT
CAACACGCGCATTCCGTCGGTGTCGGACCTTGACTTGCCGCCCACGCTTCACGAGATCGTCATGTCCAAGCGCGGCCTGG
TGATCGTGACCGGCGCCACGGGCTCGGGCAAGTCGACCACGCTCGCGGCGATGCTCGATCACCGCAATGCGCATTCGTAC
GGCCACATCATCACCATCGAGGACCCGATCGAATACGTGCATGCGCACCAGAACTGCGTCGTCACACAGCGCGAGGTGGG
CATCGATACCGAGTCCTGGCATGTGGCGCTGAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGATCGCGAGACCATGGAATACGCGATGCAGTACGCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAAC
GCCAACCAGGCGATCGACCGTATTGTCAACTTCTTCCCGGAAGAGAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTGAA
CCTGAAGGCCATGGTGTCGCAGCGGCTGCTGCCGCGAGCGGGCCGCAAGGGGCGTGTGCCGGCCGTCGAAATCATGATCG
GCACGCCGCTGGTGGCCGACCTTATCTTCAAGGGCGAGATCCACGAACTCAAGGAAGTGATCAAGAAATCGCGCGAGCAG
GGCATGATTTCGTTCGACCAGGCGTTGTTCGACCTGCACGAAAGCGGCAAGATCACGTATGAGGACGCGCTGCGCAATGC
CGATTCGCTCAATGACCTTCGGCTGATGATCAAGCTGCACAGTGCGACCAGGAAGGACAGCGATTTGGGTGCCGGCACCG
AGCACTTGAACGTTATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.143

92.348

0.583
  pilU Acinetobacter baylyi ADP1

57.382

94.723

0.544
  pilU Vibrio cholerae strain A1552

53.444

95.778

0.512
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

88.918

0.396
  pilT Pseudomonas stutzeri DSM 10701

43.323

88.918

0.385
  pilT Legionella pneumophila strain Lp02

45.031

84.96

0.383
  pilT Legionella pneumophila strain ERS1305867

45.031

84.96

0.383
  pilT Pseudomonas aeruginosa PAK

42.73

88.918

0.38
  pilT Acinetobacter baylyi ADP1

42.943

87.863

0.377
  pilT Acinetobacter baumannii strain A118

42.643

87.863

0.375
  pilT Acinetobacter baumannii D1279779

42.643

87.863

0.375
  pilT Acinetobacter nosocomialis M2

42.643

87.863

0.375
  pilT Neisseria meningitidis 8013

40.882

89.71

0.367