Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K2L43_RS02290 Genome accession   NZ_CP080761
Coordinates   464055..465098 (-) Length   347 a.a.
NCBI ID   WP_047350274.1    Uniprot ID   A0A2S4KEP4
Organism   Diaphorobacter sp. MNS-0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459055..470098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2L43_RS02260 (K2L43_02260) - 459353..459751 (+) 399 WP_135149859.1 YraN family protein -
  K2L43_RS02265 (K2L43_02265) - 459810..460403 (+) 594 WP_220638415.1 SIS domain-containing protein -
  K2L43_RS02270 (K2L43_02270) - 460400..461074 (+) 675 WP_012655453.1 BON domain-containing protein -
  K2L43_RS02275 (K2L43_02275) - 461172..462101 (-) 930 WP_011803878.1 NAD(P)-dependent oxidoreductase -
  K2L43_RS02280 (K2L43_02280) pilU 462166..463302 (-) 1137 WP_012655455.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K2L43_RS02285 (K2L43_02285) - 463349..463978 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  K2L43_RS02290 (K2L43_02290) pilT 464055..465098 (-) 1044 WP_047350274.1 type IV pilus twitching motility protein PilT Machinery gene
  K2L43_RS02295 (K2L43_02295) - 465138..465854 (+) 717 WP_220638416.1 YggS family pyridoxal phosphate-dependent enzyme -
  K2L43_RS02300 (K2L43_02300) - 465893..467029 (+) 1137 WP_220638417.1 aminotransferase class V-fold PLP-dependent enzyme -
  K2L43_RS02305 (K2L43_02305) - 467112..468458 (+) 1347 WP_187148593.1 DUF445 domain-containing protein -
  K2L43_RS02310 (K2L43_02310) - 468518..468979 (-) 462 WP_220638418.1 carboxypeptidase regulatory-like domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38176.90 Da        Isoelectric Point: 6.7742

>NTDB_id=594781 K2L43_RS02290 WP_047350274.1 464055..465098(-) (pilT) [Diaphorobacter sp. MNS-0]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKTYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPRIFADLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNESEYGHILTIED
PIEFVHESKKCLVNQREVGPMTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LSDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=594781 K2L43_RS02290 WP_047350274.1 464055..465098(-) (pilT) [Diaphorobacter sp. MNS-0]
GTGGACATCACCCAATTGCTCGCCTTCAGCGTGAAGAACAAGGCGTCCGACCTGCACCTTTCCGCCGGCCTGCCGCCCAT
GATCCGGGTCCACGGGGACGTACGCCGCATCAACGTCGATGCACTGGACCACAAGACCGTCCACGCCATGGTGTACGACA
TCATGAGCGACGCCCAGCGCAAGACCTACGAAGAGTTCCTGGAAGTGGACTTTTCCTTCGAGATCGACGGCCTGGCGCGC
TTTCGCGTCAACGCGTTCAACCAGAACCGTGGCGCGGCCGCGGTGTTCCGTACCATTCCGAGCAAGATCCTGACGCTGGA
GCAGCTCAACGCGCCGCGCATTTTTGCCGACCTGGCGCTCAAGCCACGCGGCCTGGTGCTGGTGACGGGCCCCACGGGTT
CGGGCAAGTCCACCACGCTGGCGGCCATGGTCAACTACCTCAACGAAAGCGAGTACGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCACGAGTCCAAGAAGTGCCTGGTCAACCAGCGCGAAGTGGGGCCGATGACGCTGTCGTTCGCCGC
GGCCCTGCGCTCAGCGCTGCGCGAGGACCCGGACGCCATTCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACCGCCGCCGAGACCGGCCACTTGGTGTTCGGCACGCTGCACACGTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTCTTCCCCGCTGAAGAAAAGGAAATGGTGCGCGCGATGTTGTCGGAGTCGCTGCAGGCGGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGACGGCTCGGGCCGCGTGGCGGCGCACGAGATCATGCTGGGCACGAGCGCCATCCGCAATCTGA
TCCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCAACAGCGTCGGTATGCAGACGCTGGACCAGAAC
CTCTCCGACCTGGTGCGCCGCAACATCATCAGCCCGGCCGAGGCGCGCAGCAAGGCCAAGATTCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S4KEP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.812

99.424

0.764
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Acinetobacter baylyi ADP1

74.783

99.424

0.744
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.035

98.271

0.669
  pilT Vibrio cholerae strain A1552

68.035

98.271

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406