Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K2L43_RS02280 Genome accession   NZ_CP080761
Coordinates   462166..463302 (-) Length   378 a.a.
NCBI ID   WP_012655455.1    Uniprot ID   A0A2S4KET9
Organism   Diaphorobacter sp. MNS-0     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457166..468302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K2L43_RS02250 (K2L43_02250) - 457280..458209 (+) 930 WP_220638413.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  K2L43_RS02255 (K2L43_02255) rsmI 458415..459353 (-) 939 WP_220638414.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  K2L43_RS02260 (K2L43_02260) - 459353..459751 (+) 399 WP_135149859.1 YraN family protein -
  K2L43_RS02265 (K2L43_02265) - 459810..460403 (+) 594 WP_220638415.1 SIS domain-containing protein -
  K2L43_RS02270 (K2L43_02270) - 460400..461074 (+) 675 WP_012655453.1 BON domain-containing protein -
  K2L43_RS02275 (K2L43_02275) - 461172..462101 (-) 930 WP_011803878.1 NAD(P)-dependent oxidoreductase -
  K2L43_RS02280 (K2L43_02280) pilU 462166..463302 (-) 1137 WP_012655455.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K2L43_RS02285 (K2L43_02285) - 463349..463978 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  K2L43_RS02290 (K2L43_02290) pilT 464055..465098 (-) 1044 WP_047350274.1 type IV pilus twitching motility protein PilT Machinery gene
  K2L43_RS02295 (K2L43_02295) - 465138..465854 (+) 717 WP_220638416.1 YggS family pyridoxal phosphate-dependent enzyme -
  K2L43_RS02300 (K2L43_02300) - 465893..467029 (+) 1137 WP_220638417.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41729.18 Da        Isoelectric Point: 6.9080

>NTDB_id=594780 K2L43_RS02280 WP_012655455.1 462166..463302(-) (pilU) [Diaphorobacter sp. MNS-0]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGVGRFRVNAFVQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMLDWRNENSFG
HIITVEDPVEFVHPHKNCVITQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALISQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVISYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=594780 K2L43_RS02280 WP_012655455.1 462166..463302(-) (pilU) [Diaphorobacter sp. MNS-0]
ATGGAACGGGATCAGGCGAGCAAATTCATCAACGATCTGCTCAAGCTGATGGTCAGCCGCGGGGGGAGCGACCTTTTCAT
CACGGCGGAGTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCTCCCCAGCCTCTGACGCCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTTGCCATC
TCGCCCGCGGGCGTGGGCCGGTTCCGAGTGAACGCCTTCGTACAGCAGGGCCGCGTGGGCATGGTGCTGCGGACGATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTTCCGCAGGTGCTCAAGGAAGTCACCATGACCAAGCGCGGGCTGTGCA
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGCTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCGGTGGAATTCGTGCATCCGCACAAGAACTGCGTGATCACGCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCGGCCCTCAAAAACACGCTGCGTCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGTTGCTGATGGACCTGTCGCTGAACCT
CAAGGCACTGATCTCGCAGCGCCTGATTCCCAAGCAGGACGGCAAGGGGCGCGCGGCTGCCGTCGAGGTGATGCTGAACA
CGCCGCTGATCGCCGACCTCATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGCCAACGTGATCAGCTATGAGGATGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCTTGCAGATCAAGCTCAGCAGCCAGCGTGCCAAGACGACGGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S4KET9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

58.939

94.709

0.558
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Pseudomonas aeruginosa PAK

45.994

89.153

0.41
  pilT Acinetobacter baumannii strain A118

45.946

88.095

0.405
  pilT Acinetobacter nosocomialis M2

45.946

88.095

0.405
  pilT Acinetobacter baumannii D1279779

45.946

88.095

0.405
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

44.214

89.153

0.394
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Acinetobacter baylyi ADP1

43.544

88.095

0.384
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37