Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K3G24_RS17850 Genome accession   NZ_CP080630
Coordinates   3882701..3883735 (-) Length   344 a.a.
NCBI ID   WP_107683805.1    Uniprot ID   A0A2T4N0J7
Organism   Aeromonas veronii strain A29V     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3877701..3888735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3G24_RS17820 (K3G24_17800) glnK 3878281..3878619 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  K3G24_RS17825 (K3G24_17805) arfB 3878821..3879237 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  K3G24_RS17830 (K3G24_17810) - 3879270..3879761 (+) 492 WP_107683803.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  K3G24_RS17835 (K3G24_17815) - 3880122..3880547 (-) 426 WP_005340360.1 hypothetical protein -
  K3G24_RS17840 (K3G24_17820) yaaA 3880742..3881515 (-) 774 WP_107683804.1 peroxide stress protein YaaA -
  K3G24_RS17845 (K3G24_17825) pilU 3881563..3882672 (-) 1110 WP_107683852.1 type IVa pilus ATPase TapU Machinery gene
  K3G24_RS17850 (K3G24_17830) pilT 3882701..3883735 (-) 1035 WP_107683805.1 type IVa pilus ATPase TapT Machinery gene
  K3G24_RS17855 (K3G24_17835) - 3883775..3884476 (+) 702 WP_118854299.1 YggS family pyridoxal phosphate-dependent enzyme -
  K3G24_RS17860 (K3G24_17840) proC 3884619..3885443 (+) 825 WP_107683807.1 pyrroline-5-carboxylate reductase -
  K3G24_RS17865 (K3G24_17845) - 3885455..3886006 (+) 552 WP_047436998.1 YggT family protein -
  K3G24_RS17870 (K3G24_17850) yggU 3886006..3886308 (+) 303 WP_107683808.1 DUF167 family protein YggU -
  K3G24_RS17875 (K3G24_17855) - 3886352..3886771 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  K3G24_RS17880 (K3G24_17860) - 3886873..3888069 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38259.00 Da        Isoelectric Point: 6.7439

>NTDB_id=594577 K3G24_RS17850 WP_107683805.1 3882701..3883735(-) (pilT) [Aeromonas veronii strain A29V]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINDNFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=594577 K3G24_RS17850 WP_107683805.1 3882701..3883735(-) (pilT) [Aeromonas veronii strain A29V]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCGCGCGGTTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTACTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGATAACTTCCATCACCACATTCTCACCATCGAAGAC
CCCATTGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTTCACCGGGATACCAAGAGCTTCAGCAA
CGCCCTGCGCTCGGCCCTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACCGGCCATCTGGTGTTTGGCACCTTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCAGGTGCGGAGAAGGACATGGTGCGTTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATCC
GGGAGGACAAGATCGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCCGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4N0J7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

69.275

100

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407