Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K3G24_RS17845 Genome accession   NZ_CP080630
Coordinates   3881563..3882672 (-) Length   369 a.a.
NCBI ID   WP_107683852.1    Uniprot ID   A0A2T4N0R6
Organism   Aeromonas veronii strain A29V     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3876563..3887672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K3G24_RS17815 (K3G24_17795) - 3877015..3878022 (-) 1008 WP_236095781.1 Fe(3+) ABC transporter substrate-binding protein -
  K3G24_RS17820 (K3G24_17800) glnK 3878281..3878619 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  K3G24_RS17825 (K3G24_17805) arfB 3878821..3879237 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  K3G24_RS17830 (K3G24_17810) - 3879270..3879761 (+) 492 WP_107683803.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  K3G24_RS17835 (K3G24_17815) - 3880122..3880547 (-) 426 WP_005340360.1 hypothetical protein -
  K3G24_RS17840 (K3G24_17820) yaaA 3880742..3881515 (-) 774 WP_107683804.1 peroxide stress protein YaaA -
  K3G24_RS17845 (K3G24_17825) pilU 3881563..3882672 (-) 1110 WP_107683852.1 type IVa pilus ATPase TapU Machinery gene
  K3G24_RS17850 (K3G24_17830) pilT 3882701..3883735 (-) 1035 WP_107683805.1 type IVa pilus ATPase TapT Machinery gene
  K3G24_RS17855 (K3G24_17835) - 3883775..3884476 (+) 702 WP_118854299.1 YggS family pyridoxal phosphate-dependent enzyme -
  K3G24_RS17860 (K3G24_17840) proC 3884619..3885443 (+) 825 WP_107683807.1 pyrroline-5-carboxylate reductase -
  K3G24_RS17865 (K3G24_17845) - 3885455..3886006 (+) 552 WP_047436998.1 YggT family protein -
  K3G24_RS17870 (K3G24_17850) yggU 3886006..3886308 (+) 303 WP_107683808.1 DUF167 family protein YggU -
  K3G24_RS17875 (K3G24_17855) - 3886352..3886771 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41080.10 Da        Isoelectric Point: 6.5045

>NTDB_id=594576 K3G24_RS17845 WP_107683852.1 3881563..3882672(-) (pilU) [Aeromonas veronii strain A29V]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEELQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVSQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=594576 K3G24_RS17845 WP_107683852.1 3881563..3882672(-) (pilU) [Aeromonas veronii strain A29V]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAGGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCGATCTATCGCGAGGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGACGTATCGAGACCCGCATTCCCACCTTTGA
AGAGCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGACTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACGGGACATCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTTGATCGCATC
CTCCATCTGGTGTCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCCGCGTTCGAGATCCTGCTCAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAACTCTCCGGTCGCGAACGGCTCGGCACAGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T4N0R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363