Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A9R16_RS13325 Genome accession   NZ_CP080624
Coordinates   2843989..2845059 (-) Length   356 a.a.
NCBI ID   WP_065971998.1    Uniprot ID   A0A1C2FY27
Organism   Acidiferrobacter thiooxydans strain ZJ     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2838989..2850059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9R16_RS13305 (A9R16_013465) - 2839856..2840605 (-) 750 WP_065971994.1 MBL fold metallo-hydrolase -
  A9R16_RS13310 (A9R16_013470) - 2840864..2841949 (-) 1086 WP_065971995.1 hypothetical protein -
  A9R16_RS13315 (A9R16_013475) murI 2841956..2842756 (-) 801 WP_218060518.1 glutamate racemase -
  A9R16_RS13320 (A9R16_013480) pilU 2842875..2843987 (-) 1113 WP_065971997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A9R16_RS13325 (A9R16_013485) pilT 2843989..2845059 (-) 1071 WP_065971998.1 type IV pilus twitching motility protein PilT Machinery gene
  A9R16_RS13330 (A9R16_013490) proC 2845163..2845984 (+) 822 WP_065971999.1 pyrroline-5-carboxylate reductase -
  A9R16_RS13335 (A9R16_013495) - 2845981..2846553 (+) 573 WP_083996259.1 YggT family protein -
  A9R16_RS13340 (A9R16_013500) - 2846550..2847035 (+) 486 WP_065972000.1 redoxin domain-containing protein -
  A9R16_RS13345 (A9R16_013505) - 2847045..2847224 (-) 180 WP_065972001.1 hypothetical protein -
  A9R16_RS13350 (A9R16_013510) trxA 2847339..2847752 (+) 414 WP_065972002.1 thioredoxin -
  A9R16_RS13355 (A9R16_013515) metX 2847813..2848958 (+) 1146 WP_065972003.1 homoserine O-succinyltransferase MetX -
  A9R16_RS13360 (A9R16_013520) metW 2848955..2849566 (+) 612 WP_065972004.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39420.07 Da        Isoelectric Point: 6.7723

>NTDB_id=594413 A9R16_RS13325 WP_065971998.1 2843989..2845059(-) (pilT) [Acidiferrobacter thiooxydans strain ZJ]
MDIAELLAFSYKQNASDLHLSAGLPPLIRVDGDVKRINVDPLDERAVHNMVYDIMNDKQQKEYEERLECDFSFELPNIAR
FRVNAFNQNRGPGAVFRTIPSKVLTLEQLNAPGIFKQIADQPRGIVLVTGPTGSGKSTTLAAMIDHINESRREHILTIED
PIEFVHTSKNCLINQREVHRDTLGFENALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPAAEKEMVRSMLSESLRAVISQTLMKKVGGGRIAAHEIMIGTPAIRNLIRENKIAQMYSAIQTSQSVGMQTLDQCL
MELVRSRQVSVEEARGKAANKDAFANNNGAAAAARR

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=594413 A9R16_RS13325 WP_065971998.1 2843989..2845059(-) (pilT) [Acidiferrobacter thiooxydans strain ZJ]
ATGGATATCGCGGAACTCCTGGCCTTTTCCTATAAACAGAACGCGTCGGATCTGCATTTGTCGGCCGGTTTGCCACCTCT
CATCCGTGTCGACGGCGACGTCAAGCGCATCAACGTAGACCCATTGGATGAGCGCGCCGTGCACAATATGGTCTACGATA
TCATGAACGACAAGCAGCAAAAGGAGTATGAGGAGCGGCTGGAGTGCGATTTCTCGTTCGAGCTGCCTAACATCGCGCGC
TTTCGTGTTAATGCCTTTAATCAGAATCGTGGCCCGGGCGCCGTCTTTCGGACCATTCCCTCGAAGGTGCTGACCCTCGA
GCAATTGAACGCCCCCGGAATCTTCAAGCAGATCGCCGACCAGCCGCGCGGTATCGTGCTGGTTACGGGCCCCACGGGGT
CCGGCAAATCCACCACCCTGGCGGCGATGATAGACCACATCAACGAGAGCCGACGCGAGCACATACTGACCATAGAGGAC
CCGATCGAGTTCGTGCATACGAGCAAGAACTGTCTTATCAATCAGCGCGAGGTTCACCGGGATACCCTGGGGTTCGAGAA
CGCCTTGCGTTCGGCGTTGCGCGAAGATCCGGACGTCATCCTGGTCGGCGAATTGCGCGATTTGGAGACCATCCGCCTCG
CGCTCACCGCCGCCGAGACCGGTCATCTCGTATTTGCCACCCTGCATACAAGTTCCGCGGCCAAGACCATAGACCGCGTG
GTGGACGTGTTCCCGGCGGCGGAGAAGGAGATGGTACGCTCCATGCTCTCGGAGTCGTTGCGCGCGGTGATCTCGCAGAC
GCTGATGAAGAAGGTCGGTGGCGGGCGCATCGCCGCCCACGAGATCATGATCGGCACGCCCGCCATTCGCAACCTAATTC
GCGAAAACAAGATCGCGCAGATGTATTCAGCCATTCAGACCAGCCAGTCCGTGGGTATGCAGACCCTCGATCAGTGTCTC
ATGGAGCTCGTGCGGTCGCGTCAGGTATCGGTCGAGGAGGCGCGCGGCAAGGCGGCGAACAAGGACGCATTCGCAAATAA
CAACGGGGCGGCCGCCGCCGCGCGGAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2FY27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.744

96.629

0.742
  pilT Acinetobacter nosocomialis M2

76.163

96.629

0.736
  pilT Acinetobacter baumannii D1279779

76.163

96.629

0.736
  pilT Acinetobacter baumannii strain A118

76.163

96.629

0.736
  pilT Pseudomonas stutzeri DSM 10701

75.581

96.629

0.73
  pilT Acinetobacter baylyi ADP1

73.256

96.629

0.708
  pilT Legionella pneumophila strain Lp02

70.93

96.629

0.685
  pilT Legionella pneumophila strain ERS1305867

70.93

96.629

0.685
  pilT Neisseria meningitidis 8013

68.208

97.191

0.663
  pilT Neisseria gonorrhoeae MS11

67.919

97.191

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.843

94.663

0.652
  pilT Vibrio cholerae strain A1552

68.843

94.663

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

97.191

0.492
  pilU Acinetobacter baylyi ADP1

41.964

94.382

0.396
  pilU Vibrio cholerae strain A1552

41.791

94.101

0.393
  pilU Pseudomonas stutzeri DSM 10701

41.194

94.101

0.388