Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   A9R16_RS13320 Genome accession   NZ_CP080624
Coordinates   2842875..2843987 (-) Length   370 a.a.
NCBI ID   WP_065971997.1    Uniprot ID   A0A1C2FY09
Organism   Acidiferrobacter thiooxydans strain ZJ     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2837875..2848987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A9R16_RS13305 (A9R16_013465) - 2839856..2840605 (-) 750 WP_065971994.1 MBL fold metallo-hydrolase -
  A9R16_RS13310 (A9R16_013470) - 2840864..2841949 (-) 1086 WP_065971995.1 hypothetical protein -
  A9R16_RS13315 (A9R16_013475) murI 2841956..2842756 (-) 801 WP_218060518.1 glutamate racemase -
  A9R16_RS13320 (A9R16_013480) pilU 2842875..2843987 (-) 1113 WP_065971997.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  A9R16_RS13325 (A9R16_013485) pilT 2843989..2845059 (-) 1071 WP_065971998.1 type IV pilus twitching motility protein PilT Machinery gene
  A9R16_RS13330 (A9R16_013490) proC 2845163..2845984 (+) 822 WP_065971999.1 pyrroline-5-carboxylate reductase -
  A9R16_RS13335 (A9R16_013495) - 2845981..2846553 (+) 573 WP_083996259.1 YggT family protein -
  A9R16_RS13340 (A9R16_013500) - 2846550..2847035 (+) 486 WP_065972000.1 redoxin domain-containing protein -
  A9R16_RS13345 (A9R16_013505) - 2847045..2847224 (-) 180 WP_065972001.1 hypothetical protein -
  A9R16_RS13350 (A9R16_013510) trxA 2847339..2847752 (+) 414 WP_065972002.1 thioredoxin -
  A9R16_RS13355 (A9R16_013515) metX 2847813..2848958 (+) 1146 WP_065972003.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41229.37 Da        Isoelectric Point: 6.3159

>NTDB_id=594412 A9R16_RS13320 WP_065971997.1 2842875..2843987(-) (pilU) [Acidiferrobacter thiooxydans strain ZJ]
MGFLDLLRLMVEKGASDLYITAGVAPSLRVDGKLAPVARQALTADQARQFVYSIMNEEQRSEFEETNECNFAINPQGVGR
FRVNVFRQQQRVGMVLRKIETRIPHFEELNLPPVLKEIALTKRGLVIFVGATGSGKSTSLAAMVGYRNQYSNGHIITIED
PIEYVHEHKNCIITQREVGVDTDSFETALKNTLRQAPDVILIGEVRARETMDYAIAFAETGHLCLTTLHANSANQALDRI
INFFPEDRRAQLLMDLSLNLKAVISQRLIPKKDGAGRVAAVEIMINTPLIADLILKGEIHGIKELMAKSEEAGMQTFDQA
LFGLYEAGFIDYDEAMRNADSQNDLRLRIKLHGKGAKEAVLGDSLRNITF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=594412 A9R16_RS13320 WP_065971997.1 2842875..2843987(-) (pilU) [Acidiferrobacter thiooxydans strain ZJ]
ATGGGTTTCCTCGATCTCTTGCGGCTTATGGTCGAAAAAGGCGCCTCGGACCTTTATATCACGGCCGGGGTGGCGCCCAG
CCTGCGCGTGGACGGAAAACTGGCGCCAGTGGCGCGCCAGGCGCTCACCGCAGACCAGGCCCGGCAGTTCGTGTATAGCA
TCATGAACGAGGAGCAGCGTAGCGAGTTCGAGGAGACGAACGAGTGCAATTTCGCCATCAACCCGCAGGGCGTCGGACGC
TTTCGTGTCAACGTTTTCCGGCAGCAGCAGCGGGTCGGCATGGTGTTGCGCAAGATCGAGACCCGCATTCCGCATTTCGA
GGAATTGAATCTTCCACCGGTATTGAAAGAGATTGCCCTTACCAAGCGCGGACTCGTGATATTCGTAGGCGCCACGGGTT
CCGGAAAGTCGACCTCGCTTGCCGCCATGGTGGGCTACCGCAATCAGTATTCGAACGGGCATATCATCACCATCGAGGAC
CCGATCGAGTATGTGCACGAGCACAAGAATTGCATCATCACCCAGCGCGAGGTGGGCGTCGACACGGATTCGTTCGAGAC
AGCCCTGAAGAACACCCTGCGTCAGGCGCCCGATGTGATCCTCATAGGCGAGGTGCGCGCGCGTGAGACGATGGACTATG
CGATCGCGTTTGCCGAGACCGGACACCTGTGTCTTACCACGCTCCATGCCAATAGCGCGAACCAGGCCCTTGATCGCATC
ATCAATTTCTTTCCGGAAGATCGGCGCGCACAGCTCCTGATGGATCTCTCCTTGAACCTGAAGGCCGTGATCTCGCAGCG
CCTCATTCCGAAGAAGGACGGTGCCGGGCGCGTGGCGGCAGTCGAGATCATGATCAACACCCCGTTGATCGCCGATCTCA
TCCTGAAGGGGGAAATTCACGGTATCAAGGAGCTCATGGCCAAATCCGAGGAGGCGGGCATGCAGACCTTCGATCAGGCA
CTGTTCGGTTTGTATGAGGCGGGCTTCATAGATTACGACGAGGCTATGCGCAACGCCGATTCGCAAAACGACCTGCGGCT
GCGCATCAAGCTGCACGGCAAGGGTGCGAAAGAGGCGGTACTCGGTGACTCCCTGCGCAACATCACTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C2FY09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.091

94.865

0.646
  pilU Acinetobacter baylyi ADP1

63.966

96.757

0.619
  pilU Vibrio cholerae strain A1552

57.478

92.162

0.53
  pilT Pseudomonas aeruginosa PAK

45.133

91.622

0.414
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.373

90.541

0.411
  pilT Acinetobacter nosocomialis M2

44.444

92.432

0.411
  pilT Acinetobacter baumannii D1279779

44.444

92.432

0.411
  pilT Acinetobacter baumannii strain A118

44.444

92.432

0.411
  pilT Acinetobacter baylyi ADP1

44.282

92.162

0.408
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.21

90.27

0.408
  pilT Vibrio cholerae strain A1552

45.21

90.27

0.408
  pilT Pseudomonas stutzeri DSM 10701

44.543

91.622

0.408
  pilT Legionella pneumophila strain Lp02

43.881

90.541

0.397
  pilT Legionella pneumophila strain ERS1305867

43.881

90.541

0.397
  pilT Neisseria meningitidis 8013

40.58

93.243

0.378
  pilT Neisseria gonorrhoeae MS11

40.58

93.243

0.378