Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K1Y48_RS07595 Genome accession   NZ_CP080536
Coordinates   1510314..1511363 (+) Length   349 a.a.
NCBI ID   WP_064219958.1    Uniprot ID   A0A179B867
Organism   Acidithiobacillus ferriphilus strain GT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1505314..1516363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1Y48_RS07565 (K1Y48_07590) - 1506507..1507076 (+) 570 WP_215848931.1 acyloxyacyl hydrolase -
  K1Y48_RS07570 (K1Y48_07595) bioB 1507118..1508068 (-) 951 WP_215848932.1 biotin synthase BioB -
  K1Y48_RS07575 (K1Y48_07600) - 1508065..1508415 (-) 351 WP_064219962.1 DUF167 domain-containing protein -
  K1Y48_RS07580 (K1Y48_07605) - 1508443..1508721 (-) 279 WP_064219961.1 YggT family protein -
  K1Y48_RS07585 (K1Y48_07610) proC 1508718..1509545 (-) 828 WP_215848933.1 pyrroline-5-carboxylate reductase -
  K1Y48_RS07590 (K1Y48_07615) - 1509542..1510213 (-) 672 WP_215848934.1 YggS family pyridoxal phosphate-dependent enzyme -
  K1Y48_RS07595 (K1Y48_07620) pilT 1510314..1511363 (+) 1050 WP_064219958.1 type IV pilus twitching motility protein PilT Machinery gene
  K1Y48_RS07600 (K1Y48_07625) pilU 1511373..1512503 (+) 1131 WP_215848935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1Y48_RS07605 (K1Y48_07630) - 1512485..1513672 (+) 1188 WP_229065903.1 glycosyltransferase -
  K1Y48_RS07610 (K1Y48_07635) - 1513861..1514248 (-) 388 Protein_1512 type II toxin-antitoxin system YhaV family toxin -
  K1Y48_RS07615 (K1Y48_07640) - 1514326..1515150 (-) 825 WP_215848936.1 abortive infection family protein -
  K1Y48_RS07620 (K1Y48_07645) - 1515238..1516032 (-) 795 WP_215848937.1 hypothetical protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38466.03 Da        Isoelectric Point: 6.7267

>NTDB_id=593615 K1Y48_RS07595 WP_064219958.1 1510314..1511363(+) (pilT) [Acidithiobacillus ferriphilus strain GT2]
MDISELLAFAVKNNASDTHISAGLSPMLRINGDIRPLNVEAQDRKAVHGMIYDIMNDSQRKGYEENLEIDFAYELPGVAR
FRVNAFNQDRGPAAAFRTIPAKVLSLEDLNAPKSFMEIINVPRGLVLVTGPTGSGKSTTLAAMVDHINANRADHIITIED
PIEFLHTPKKCLVNQREVGANTHSFEHALRSALREDPDIILVGELRDLETMRLALTAAETGHIVFATLHTSSAPKTIDRI
VDSFPGGEKDMVRAMLSESLRAVISQTLLKTADGKGRVAAHEIMIATPAIRNLIRENKVAQMYSVIQTGQNQGMQTLDQC
LADLVRAHKVTREDALRRVQNKDSFMNVA

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=593615 K1Y48_RS07595 WP_064219958.1 1510314..1511363(+) (pilT) [Acidithiobacillus ferriphilus strain GT2]
ATGGACATTTCAGAACTGCTTGCTTTCGCGGTGAAAAACAATGCTTCGGACACGCATATCTCGGCGGGTTTGTCGCCCAT
GCTGCGGATCAACGGCGATATCCGCCCTCTCAATGTCGAGGCGCAGGACAGAAAGGCCGTACATGGCATGATTTACGACA
TCATGAATGATTCCCAGCGGAAGGGGTATGAGGAAAATCTGGAAATCGACTTTGCCTATGAACTGCCCGGCGTGGCGCGT
TTTCGGGTTAACGCCTTCAACCAGGACCGTGGGCCCGCGGCGGCGTTCCGGACGATTCCCGCCAAGGTGCTGAGTCTGGA
AGATCTCAATGCACCCAAGTCTTTTATGGAGATCATCAACGTACCGCGCGGACTGGTGCTGGTCACCGGGCCGACGGGGT
CGGGTAAATCAACGACGCTGGCAGCGATGGTGGATCATATCAATGCGAATCGCGCCGACCATATTATCACCATTGAAGAT
CCCATCGAATTTTTGCACACTCCCAAGAAGTGTCTCGTCAACCAGCGCGAGGTAGGCGCCAACACTCATTCCTTTGAACA
TGCCCTGCGCTCAGCTTTACGTGAAGATCCGGATATTATTCTGGTGGGCGAATTGCGTGACTTAGAGACCATGCGCCTGG
CTCTGACTGCGGCGGAAACGGGCCATATCGTGTTTGCGACGCTGCATACCAGTTCGGCGCCGAAAACCATCGACCGTATT
GTCGACTCCTTTCCTGGGGGGGAAAAGGATATGGTTCGCGCGATGCTGTCAGAATCCCTGCGCGCGGTTATTTCTCAGAC
CCTGCTCAAAACGGCCGATGGTAAAGGCCGGGTCGCCGCGCATGAAATTATGATTGCCACACCGGCCATCCGAAACCTCA
TTCGCGAAAATAAGGTGGCGCAGATGTACTCGGTGATCCAGACCGGACAAAATCAGGGTATGCAAACTCTCGACCAGTGC
CTTGCCGACCTGGTGCGTGCCCACAAGGTAACCCGCGAAGATGCCTTGCGTAGGGTTCAGAACAAAGACAGCTTTATGAA
TGTGGCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A179B867

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

68.497

99.14

0.679
  pilT Acinetobacter baumannii D1279779

68.497

99.14

0.679
  pilT Acinetobacter baumannii strain A118

68.497

99.14

0.679
  pilT Pseudomonas stutzeri DSM 10701

68.406

98.854

0.676
  pilT Pseudomonas aeruginosa PAK

67.536

98.854

0.668
  pilT Acinetobacter baylyi ADP1

70.462

93.123

0.656
  pilT Legionella pneumophila strain Lp02

65.797

98.854

0.65
  pilT Legionella pneumophila strain ERS1305867

65.797

98.854

0.65
  pilT Neisseria meningitidis 8013

65.217

98.854

0.645
  pilT Neisseria gonorrhoeae MS11

64.928

98.854

0.642
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.308

93.123

0.636
  pilT Vibrio cholerae strain A1552

68.308

93.123

0.636
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.57

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.56

96.275

0.41
  pilU Acinetobacter baylyi ADP1

42.308

96.848

0.41
  pilU Vibrio cholerae strain A1552

39.881

96.275

0.384