Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K1Y48_RS07600 Genome accession   NZ_CP080536
Coordinates   1511373..1512503 (+) Length   376 a.a.
NCBI ID   WP_215848935.1    Uniprot ID   -
Organism   Acidithiobacillus ferriphilus strain GT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1506373..1517503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1Y48_RS07565 (K1Y48_07590) - 1506507..1507076 (+) 570 WP_215848931.1 acyloxyacyl hydrolase -
  K1Y48_RS07570 (K1Y48_07595) bioB 1507118..1508068 (-) 951 WP_215848932.1 biotin synthase BioB -
  K1Y48_RS07575 (K1Y48_07600) - 1508065..1508415 (-) 351 WP_064219962.1 DUF167 domain-containing protein -
  K1Y48_RS07580 (K1Y48_07605) - 1508443..1508721 (-) 279 WP_064219961.1 YggT family protein -
  K1Y48_RS07585 (K1Y48_07610) proC 1508718..1509545 (-) 828 WP_215848933.1 pyrroline-5-carboxylate reductase -
  K1Y48_RS07590 (K1Y48_07615) - 1509542..1510213 (-) 672 WP_215848934.1 YggS family pyridoxal phosphate-dependent enzyme -
  K1Y48_RS07595 (K1Y48_07620) pilT 1510314..1511363 (+) 1050 WP_064219958.1 type IV pilus twitching motility protein PilT Machinery gene
  K1Y48_RS07600 (K1Y48_07625) pilU 1511373..1512503 (+) 1131 WP_215848935.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1Y48_RS07605 (K1Y48_07630) - 1512485..1513672 (+) 1188 WP_229065903.1 glycosyltransferase -
  K1Y48_RS07610 (K1Y48_07635) - 1513861..1514248 (-) 388 Protein_1512 type II toxin-antitoxin system YhaV family toxin -
  K1Y48_RS07615 (K1Y48_07640) - 1514326..1515150 (-) 825 WP_215848936.1 abortive infection family protein -
  K1Y48_RS07620 (K1Y48_07645) - 1515238..1516032 (-) 795 WP_215848937.1 hypothetical protein -
  K1Y48_RS07625 (K1Y48_07650) - 1516231..1516575 (-) 345 WP_215889268.1 DUF86 domain-containing protein -
  K1Y48_RS07630 (K1Y48_07655) - 1516572..1516862 (-) 291 WP_123101710.1 nucleotidyltransferase family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41649.06 Da        Isoelectric Point: 5.7767

>NTDB_id=593616 K1Y48_RS07600 WP_215848935.1 1511373..1512503(+) (pilU) [Acidithiobacillus ferriphilus strain GT2]
MSVLDDLLVLMVEKNGSDLYLTVGSPPVMKIDGRAVSLGTEMLKPSQVLNLAKEILGMERLQEFQREKEINMAISAPGIG
RFRVNGFFQRGELSFVLRAIKTQIPSLEQLRLPPILKDLAMTSRGLVLFVGATGSGKSTSLASMIQYRNQNAAGHILTIE
DPIEFLHKNAMSIVNQREVGIDTLNYERALENALREAPDVILIGEVRSRDTMDHAMAYAETGHLCMSTLHANNANQAIER
IINFFPEDRKKQLLMDLSLNLRAVVSQRLLPIKGQGGRVAAIEVLINTPAIADLIYKGEVGLLKDAMARTNDVGMQTFDQ
SLRQLFMDGLVEYEDALRGADSQNDLRLAIKQECMRRGVEDPGASTSAEGQWRIQS

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=593616 K1Y48_RS07600 WP_215848935.1 1511373..1512503(+) (pilU) [Acidithiobacillus ferriphilus strain GT2]
ATGTCTGTGCTGGATGATCTGCTGGTCTTGATGGTGGAAAAAAATGGCTCTGACCTTTACCTCACCGTAGGCTCGCCGCC
GGTCATGAAAATTGACGGGAGGGCGGTTTCGTTAGGGACCGAGATGCTGAAGCCGAGTCAGGTGCTGAATCTGGCCAAAG
AAATTCTGGGCATGGAGCGCTTGCAGGAATTCCAGCGGGAAAAAGAAATTAATATGGCCATCTCTGCGCCCGGGATTGGG
CGTTTCCGGGTCAATGGCTTTTTTCAGCGCGGTGAACTGAGCTTTGTCTTGCGCGCCATCAAGACGCAAATTCCCAGCCT
GGAACAGCTCAGGCTGCCGCCCATACTGAAAGACTTGGCCATGACTTCCCGAGGTCTGGTGCTCTTCGTCGGGGCTACCG
GCTCTGGTAAAAGTACTTCTCTGGCATCGATGATTCAGTATCGCAATCAGAATGCGGCGGGCCATATTCTCACGATTGAG
GACCCCATCGAATTTCTTCACAAGAATGCGATGTCTATCGTTAATCAACGCGAAGTGGGTATTGATACCCTGAATTACGA
GCGGGCGCTGGAGAACGCCTTGCGTGAGGCGCCAGATGTTATTCTGATCGGCGAAGTGCGCAGTCGGGATACCATGGATC
ATGCCATGGCGTATGCAGAAACCGGACACTTGTGTATGTCGACCTTACATGCGAACAATGCGAATCAGGCCATTGAACGG
ATTATCAACTTTTTCCCGGAGGACCGTAAAAAACAATTGTTGATGGATCTGTCACTCAATTTGCGCGCCGTGGTCTCACA
ACGCCTGTTGCCGATCAAAGGGCAGGGCGGGCGCGTCGCCGCGATAGAGGTGCTGATCAATACCCCGGCCATTGCCGATT
TGATCTATAAGGGTGAAGTGGGCTTGCTGAAAGACGCCATGGCGCGGACGAATGATGTGGGTATGCAGACGTTTGATCAA
AGCCTGCGGCAACTGTTTATGGATGGACTGGTGGAATATGAAGACGCCCTGCGCGGCGCCGATTCGCAGAATGATCTGCG
CCTTGCCATCAAGCAGGAGTGCATGCGACGTGGCGTAGAGGACCCTGGTGCCAGTACTTCTGCAGAAGGGCAGTGGCGCA
TTCAGTCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.345

92.553

0.559
  pilU Vibrio cholerae strain A1552

52.893

96.543

0.511
  pilU Acinetobacter baylyi ADP1

54.441

92.819

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.228

92.287

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.848

87.766

0.394
  pilT Vibrio cholerae strain A1552

44.848

87.766

0.394
  pilT Legionella pneumophila strain Lp02

42.09

89.096

0.375
  pilT Legionella pneumophila strain ERS1305867

42.09

89.096

0.375
  pilT Acinetobacter baylyi ADP1

42.342

88.564

0.375
  pilT Acinetobacter baumannii D1279779

40.653

89.628

0.364
  pilT Acinetobacter baumannii strain A118

40.653

89.628

0.364
  pilT Acinetobacter nosocomialis M2

40.653

89.628

0.364
  pilT Pseudomonas aeruginosa PAK

41.141

88.564

0.364
  pilT Neisseria meningitidis 8013

39.535

91.489

0.362