Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K1T74_RS12615 Genome accession   NZ_CP080462
Coordinates   2755024..2756061 (-) Length   345 a.a.
NCBI ID   WP_000350195.1    Uniprot ID   Q9KUQ3
Organism   Vibrio cholerae strain ICDC-VC702     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2750024..2761061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1T74_RS12590 - 2750521..2751084 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  K1T74_RS12595 ruvX 2751151..2751573 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  K1T74_RS12600 tyrS 2751598..2752878 (-) 1281 WP_001088149.1 tyrosine--tRNA ligase -
  K1T74_RS12605 - 2752987..2753784 (-) 798 WP_001123220.1 helix-turn-helix transcriptional regulator -
  K1T74_RS12610 pilU 2753903..2755009 (-) 1107 WP_114743228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1T74_RS12615 pilT 2755024..2756061 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  K1T74_RS12620 - 2756087..2756797 (+) 711 WP_071192818.1 YggS family pyridoxal phosphate-dependent enzyme -
  K1T74_RS12625 proC 2756850..2757668 (+) 819 WP_053034663.1 pyrroline-5-carboxylate reductase -
  K1T74_RS12630 - 2757722..2758279 (+) 558 WP_220105833.1 YggT family protein -
  K1T74_RS12635 yggU 2758279..2758572 (+) 294 WP_000741913.1 DUF167 family protein YggU -
  K1T74_RS12640 - 2758666..2759097 (+) 432 WP_220105834.1 DUF4426 domain-containing protein -
  K1T74_RS12645 - 2759167..2759769 (+) 603 WP_000725016.1 XTP/dITP diphosphatase -
  K1T74_RS12650 hemW 2759763..2760938 (+) 1176 WP_220105835.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.83 Da        Isoelectric Point: 6.6672

>NTDB_id=593068 K1T74_RS12615 WP_000350195.1 2755024..2756061(-) (pilT) [Vibrio cholerae strain ICDC-VC702]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=593068 K1T74_RS12615 WP_000350195.1 2755024..2756061(-) (pilT) [Vibrio cholerae strain ICDC-VC702]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTACCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGTTAGA
GCAACTGGATGCTCCAGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGGT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGCTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATTTTGGTTGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCGACGCCGGCAATTCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KUQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilT Vibrio cholerae strain A1552

100

100

1
  pilT Acinetobacter baumannii D1279779

71.726

97.391

0.699
  pilT Acinetobacter baumannii strain A118

71.726

97.391

0.699
  pilT Acinetobacter nosocomialis M2

71.429

97.391

0.696
  pilT Acinetobacter baylyi ADP1

72.424

95.652

0.693
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.477

95.362

0.643
  pilT Legionella pneumophila strain ERS1305867

67.477

95.362

0.643
  pilT Neisseria meningitidis 8013

64.545

95.652

0.617
  pilT Neisseria gonorrhoeae MS11

64.242

95.652

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377