Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K1T74_RS12610 Genome accession   NZ_CP080462
Coordinates   2753903..2755009 (-) Length   368 a.a.
NCBI ID   WP_114743228.1    Uniprot ID   -
Organism   Vibrio cholerae strain ICDC-VC702     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748903..2760009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K1T74_RS12585 gshB 2749529..2750485 (+) 957 WP_000593313.1 glutathione synthase -
  K1T74_RS12590 - 2750521..2751084 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  K1T74_RS12595 ruvX 2751151..2751573 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  K1T74_RS12600 tyrS 2751598..2752878 (-) 1281 WP_001088149.1 tyrosine--tRNA ligase -
  K1T74_RS12605 - 2752987..2753784 (-) 798 WP_001123220.1 helix-turn-helix transcriptional regulator -
  K1T74_RS12610 pilU 2753903..2755009 (-) 1107 WP_114743228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K1T74_RS12615 pilT 2755024..2756061 (-) 1038 WP_000350195.1 type IV pilus twitching motility protein PilT Machinery gene
  K1T74_RS12620 - 2756087..2756797 (+) 711 WP_071192818.1 YggS family pyridoxal phosphate-dependent enzyme -
  K1T74_RS12625 proC 2756850..2757668 (+) 819 WP_053034663.1 pyrroline-5-carboxylate reductase -
  K1T74_RS12630 - 2757722..2758279 (+) 558 WP_220105833.1 YggT family protein -
  K1T74_RS12635 yggU 2758279..2758572 (+) 294 WP_000741913.1 DUF167 family protein YggU -
  K1T74_RS12640 - 2758666..2759097 (+) 432 WP_220105834.1 DUF4426 domain-containing protein -
  K1T74_RS12645 - 2759167..2759769 (+) 603 WP_000725016.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41277.24 Da        Isoelectric Point: 6.5076

>NTDB_id=593067 K1T74_RS12610 WP_114743228.1 2753903..2755009(-) (pilU) [Vibrio cholerae strain ICDC-VC702]
MELNQYLDGMLAHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEVLLNSPRIADLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=593067 K1T74_RS12610 WP_114743228.1 2753903..2755009(-) (pilU) [Vibrio cholerae strain ICDC-VC702]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGGCTCATAAAGCATCGGATCTGTACATTACGGTGGGTGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCACAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
CAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTTGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCCATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGTGAAATTCGCAGCCGAGAAACCATGGAATACGCAATGA
CTTTCGCGGAAACGGGTCATCTGTGTATGGCCACGCTGCACGCCAACAACGCTAACCAAGCATTAGAGCGGATTTTGCAT
CTTGTGCCAAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCCCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAGTGTTGCTCAATAGCCCGCGTATCGCGGATTTAATTCGCC
GCGGTGAACTGCATGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACTTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGCGGCTCTTTGCAGAATGTGAAGATTGATATGGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.728

100

0.997
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.806

84.239

0.386
  pilT Vibrio cholerae strain A1552

42.216

90.761

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

90.761

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

42.462

88.315

0.375
  pilT Neisseria gonorrhoeae MS11

38.329

94.293

0.361
  pilT Neisseria meningitidis 8013

38.551

93.75

0.361