Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0I73_RS13500 Genome accession   NZ_CP080421
Coordinates   3043919..3044956 (-) Length   345 a.a.
NCBI ID   WP_220061599.1    Uniprot ID   -
Organism   Shewanella mesophila strain FJAT-53870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038919..3049956
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I73_RS13470 (K0I73_13470) yciH 3039151..3039480 (+) 330 WP_220064386.1 stress response translation initiation inhibitor YciH -
  K0I73_RS13475 (K0I73_13475) - 3039499..3040056 (+) 558 WP_220061594.1 YqgE/AlgH family protein -
  K0I73_RS13480 (K0I73_13480) ruvX 3040074..3040493 (+) 420 WP_220061595.1 Holliday junction resolvase RuvX -
  K0I73_RS13485 (K0I73_13485) hemH 3041070..3042086 (-) 1017 WP_220061596.1 ferrochelatase -
  K0I73_RS13490 (K0I73_13490) - 3042111..3042653 (-) 543 WP_220061597.1 glutathione peroxidase -
  K0I73_RS13495 (K0I73_13495) pilU 3042793..3043905 (-) 1113 WP_220061598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0I73_RS13500 (K0I73_13500) pilT 3043919..3044956 (-) 1038 WP_220061599.1 type IV pilus twitching motility protein PilT Machinery gene
  K0I73_RS13505 (K0I73_13505) - 3044992..3045702 (+) 711 WP_220061600.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0I73_RS13510 (K0I73_13510) proC 3045775..3046593 (+) 819 WP_220061601.1 pyrroline-5-carboxylate reductase -
  K0I73_RS13515 (K0I73_13515) - 3046631..3047179 (+) 549 WP_220061602.1 YggT family protein -
  K0I73_RS13520 (K0I73_13520) yggU 3047179..3047466 (+) 288 WP_220061603.1 DUF167 family protein YggU -
  K0I73_RS13525 (K0I73_13525) - 3047557..3047991 (+) 435 WP_220061604.1 DUF4426 domain-containing protein -
  K0I73_RS13530 (K0I73_13530) rdgB 3048132..3048734 (+) 603 WP_220061605.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0I73_RS13535 (K0I73_13535) hemW 3048734..3049873 (+) 1140 WP_220061606.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.83 Da        Isoelectric Point: 6.9097

>NTDB_id=592838 K0I73_RS13500 WP_220061599.1 3043919..3044956(-) (pilT) [Shewanella mesophila strain FJAT-53870]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMSKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592838 K0I73_RS13500 WP_220061599.1 3043919..3044956(-) (pilT) [Shewanella mesophila strain FJAT-53870]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAGCACAATGCGTCAGATCTACACCTTTCTGCGGGAGTATCTCCAAT
GATACGTGTAGATGGTGAGGTCAGAAAGATTAATCTGCCTGCATTAGATCATCAAGGTGTGCATAGTCTTGTTTATGACA
TCATGAATGATAAGCAGCGTAAAGATTTTGAAGAACATCTCGAGATCGATTTCTCGTTCGAAGTTCCTAATTTAGCCCGT
TTTCGTGTCAATGCGTTTAACCAGTCTCGTGGTGCCGCCGCGGTATTTCGTACTATTCCAAGTGAGATCTTGAGTCTTGA
AAAACTGGGCGCACCCGAAATTTTTAAAAAGATCTCCAGTTTCCCTCGCGGATTAGTGCTGGTAACAGGCCCCACAGGTT
CGGGTAAGAGTACCACTCTGGCCGCTATGGTCGATTACGTGAATGAGAATCGCCATGATCATATTTTGACCATTGAAGAC
CCTATCGAATTTGTCCACCAGAACAAGCAATGTCTAATCAACCAGCGTGAAGTGCACAGACACACCCACAGCTTCAACGC
CGCGCTGAGAAGTGCGCTGCGTGAAGATCCGGATGTGATCCTTGTGGGTGAGATGCGTGACCTTGAAACCATTAGACTGG
CGATGACGGCGGCAGAAACGGGTCACTTAGTTTTTGGTACCCTACATACCACCTCAGCGGCGAAAACCATTGACCGTGTG
GTCGACGTTTTCCCCGAAGGCGAGAAGGGCATGGTCAGAACCATGTTATCTGAATCGCTGCAAGCGGTTATCTCGCAAAC
CCTCATTAAGAAAGTGGGTGGGGGGCGAGTTGCCGCACATGAGATCATGATGGGAACGCCTGCGATCCGTAACCTTATTC
GTGAAGATAAGGTAGCGCAGATGTACTCGGCAATTCAGACTGGTATGGCTCATGGAATGCAGACACTCGATCAGTGTTTA
CAAAATTTAGTTAATCGAGGGCTTATCACTCGCGAAGACGCGATGTCTAAGAGTTCGAACAAGAATGCTAGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.138

96.812

0.786
  pilT Acinetobacter baumannii D1279779

81.138

96.812

0.786
  pilT Acinetobacter baumannii strain A118

81.138

96.812

0.786
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

78.698

97.971

0.771
  pilT Acinetobacter baylyi ADP1

79.635

95.362

0.759
  pilT Legionella pneumophila strain Lp02

74.269

99.13

0.736
  pilT Legionella pneumophila strain ERS1305867

74.269

99.13

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.261

0.493
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.42

0.412
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

39.244

99.71

0.391