Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0I73_RS13495 Genome accession   NZ_CP080421
Coordinates   3042793..3043905 (-) Length   370 a.a.
NCBI ID   WP_220061598.1    Uniprot ID   -
Organism   Shewanella mesophila strain FJAT-53870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3037793..3048905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I73_RS13460 (K0I73_13460) - 3037886..3038446 (-) 561 WP_220061592.1 DUF4136 domain-containing protein -
  K0I73_RS13465 (K0I73_13465) - 3038622..3038936 (+) 315 WP_220061593.1 hypothetical protein -
  K0I73_RS13470 (K0I73_13470) yciH 3039151..3039480 (+) 330 WP_220064386.1 stress response translation initiation inhibitor YciH -
  K0I73_RS13475 (K0I73_13475) - 3039499..3040056 (+) 558 WP_220061594.1 YqgE/AlgH family protein -
  K0I73_RS13480 (K0I73_13480) ruvX 3040074..3040493 (+) 420 WP_220061595.1 Holliday junction resolvase RuvX -
  K0I73_RS13485 (K0I73_13485) hemH 3041070..3042086 (-) 1017 WP_220061596.1 ferrochelatase -
  K0I73_RS13490 (K0I73_13490) - 3042111..3042653 (-) 543 WP_220061597.1 glutathione peroxidase -
  K0I73_RS13495 (K0I73_13495) pilU 3042793..3043905 (-) 1113 WP_220061598.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0I73_RS13500 (K0I73_13500) pilT 3043919..3044956 (-) 1038 WP_220061599.1 type IV pilus twitching motility protein PilT Machinery gene
  K0I73_RS13505 (K0I73_13505) - 3044992..3045702 (+) 711 WP_220061600.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0I73_RS13510 (K0I73_13510) proC 3045775..3046593 (+) 819 WP_220061601.1 pyrroline-5-carboxylate reductase -
  K0I73_RS13515 (K0I73_13515) - 3046631..3047179 (+) 549 WP_220061602.1 YggT family protein -
  K0I73_RS13520 (K0I73_13520) yggU 3047179..3047466 (+) 288 WP_220061603.1 DUF167 family protein YggU -
  K0I73_RS13525 (K0I73_13525) - 3047557..3047991 (+) 435 WP_220061604.1 DUF4426 domain-containing protein -
  K0I73_RS13530 (K0I73_13530) rdgB 3048132..3048734 (+) 603 WP_220061605.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41315.40 Da        Isoelectric Point: 6.1403

>NTDB_id=592837 K0I73_RS13495 WP_220061598.1 3042793..3043905(-) (pilU) [Shewanella mesophila strain FJAT-53870]
MEVRPFLKVMTDRKASDLFITAGFPPSAKIDGEVRPLGENAFTPEQSLEFVESLMTEAQRKEFHESRECNFAYAAKDLGR
FRVSAFWQRESPGCVMRRIETKIPEVEELKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRIASLIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYLENEISYADALHYADSPNDLRLMIKLQSKEAASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592837 K0I73_RS13495 WP_220061598.1 3042793..3043905(-) (pilU) [Shewanella mesophila strain FJAT-53870]
ATGGAAGTTCGTCCATTTTTAAAAGTAATGACAGATCGTAAAGCATCGGATCTATTTATTACCGCAGGTTTTCCGCCTAG
CGCCAAAATTGACGGTGAGGTGAGGCCGCTAGGGGAAAACGCATTTACGCCGGAGCAGTCATTAGAGTTTGTTGAATCGT
TAATGACAGAGGCTCAACGTAAAGAGTTTCATGAAAGCCGCGAATGTAACTTTGCTTATGCCGCTAAGGATCTTGGTCGT
TTTCGCGTGAGTGCTTTTTGGCAGCGTGAATCTCCTGGCTGCGTAATGCGCCGTATTGAGACCAAAATACCTGAGGTTGA
AGAATTAAAGCTACCACCGATCTTAAAAGATTTGGTGATGAGTAAACGCGGTCTGATCATCATGGTGGGTGGTACGGGTA
CCGGTAAGTCGACTTCACTTGCCGCGCTAGTCGGTTATCGAAATGCCCATGCTCGCGGACATATTTTAACCATCGAAGAT
CCAGTCGAGTTTGTTCATGATCACCGCAAGAGTATTATTACTCAGCGTGAAGTGGGTATTGATACCGAGTCGTTTGATGC
GGCGCTGAAAAGTTCTCTGCGTCAGGCACCCGACGTGATTTTGATTGGTGAGATCCGTAGCCAAGAAACCATGGAGTTTG
CCCTCTCCTTCGCGGAAACGGGGCACCTGTGTATGGCCACCTTGCACGCCAACAACGCTAACCAAGCGTTGGATCGTATT
ATGCATTTAGTGCCAGAGAGTAAGCATCAACAGCTGTTGTTCGATCTGTCTCTTAACCTTCGTGGTATTGTCGCCCAACA
ACTGGTCCCTAAGAGCGATGGCAGCGGTCGAAGAGCTGCCATTGAGGTATTGATCAACACGCCTCGTATTGCCAGTCTTA
TTCAGAAAAATGAGCTACACCTGCTAAAAGAAACCATGGCTAAATCTAATGAGCAGGGGATGCAGACATTTGATCAGGCC
TTGCTTAAGCTTTATTTAGAGAATGAGATTAGTTATGCAGATGCACTGCACTATGCCGATTCTCCAAATGATCTGCGCTT
AATGATTAAACTGCAGAGTAAAGAAGCCGCAAGTTCAGGCTTTATGGAAGGGGTGACCTTAGATCTCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Vibrio cholerae strain A1552

58.919

100

0.589
  pilU Acinetobacter baylyi ADP1

60.224

96.486

0.581
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.514

86.216

0.384
  pilT Vibrio cholerae strain A1552

44.514

86.216

0.384
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.159

93.243

0.384
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362