Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0I62_RS14340 Genome accession   NZ_CP080419
Coordinates   3238039..3239076 (-) Length   345 a.a.
NCBI ID   WP_220068759.1    Uniprot ID   -
Organism   Shewanella psychrotolerans strain FJAT-53749     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3233039..3244076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I62_RS14320 (K0I62_14320) - 3233741..3234688 (+) 948 WP_220063398.1 ribose-phosphate pyrophosphokinase -
  K0I62_RS14325 (K0I62_14325) hemH 3235204..3236220 (-) 1017 WP_220068756.1 ferrochelatase -
  K0I62_RS14330 (K0I62_14330) - 3236245..3236787 (-) 543 WP_220068757.1 glutathione peroxidase -
  K0I62_RS14335 (K0I62_14335) pilU 3236913..3238025 (-) 1113 WP_220068758.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0I62_RS14340 (K0I62_14340) pilT 3238039..3239076 (-) 1038 WP_220068759.1 type IV pilus twitching motility protein PilT Machinery gene
  K0I62_RS14345 (K0I62_14345) - 3239112..3239822 (+) 711 WP_220068760.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0I62_RS14350 (K0I62_14350) proC 3239895..3240713 (+) 819 WP_220068761.1 pyrroline-5-carboxylate reductase -
  K0I62_RS14355 (K0I62_14355) - 3240751..3241299 (+) 549 WP_220068762.1 YggT family protein -
  K0I62_RS14360 (K0I62_14360) yggU 3241299..3241586 (+) 288 WP_220068763.1 DUF167 family protein YggU -
  K0I62_RS14365 (K0I62_14365) - 3241679..3242113 (+) 435 WP_220068764.1 DUF4426 domain-containing protein -
  K0I62_RS14370 (K0I62_14370) rdgB 3242254..3242856 (+) 603 WP_220068765.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0I62_RS14375 (K0I62_14375) hemW 3242856..3243995 (+) 1140 WP_220068766.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.83 Da        Isoelectric Point: 6.9097

>NTDB_id=592787 K0I62_RS14340 WP_220068759.1 3238039..3239076(-) (pilT) [Shewanella psychrotolerans strain FJAT-53749]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDFEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAGMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMSKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592787 K0I62_RS14340 WP_220068759.1 3238039..3239076(-) (pilT) [Shewanella psychrotolerans strain FJAT-53749]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAGCACAATGCGTCAGATCTACACCTTTCTGCGGGAGTATCTCCAAT
GATACGTGTAGATGGTGAGGTCAGAAAGATTAATCTGCCTGCATTAGATCATCAAGGTGTGCATAGTCTCGTTTATGACA
TCATGAATGATAAGCAGCGCAAAGATTTTGAGGAACATCTTGAGATCGATTTCTCGTTCGAAGTGCCTAATTTAGCCCGT
TTTCGTGTTAATGCTTTTAACCAGTCTCGCGGTGCCGCCGCGGTATTTCGTACGATTCCAAGTGAGATCTTGAGTCTCGA
GAAACTGGGAGCGCCAGAAATTTTCAAAAAAATCGCTAGTTTCCCTCGTGGACTAGTGCTGGTTACGGGCCCAACGGGAT
CGGGTAAGAGTACCACCTTGGCGGGGATGGTCGATTATGTGAACGAGAATCGCCATGACCATATTTTGACCATTGAAGAC
CCTATCGAATTTGTCCACCAAAACAAACAATGTTTGATTAACCAACGGGAAGTACATCGTCATACTCACAGCTTTAATGC
TGCACTAAGAAGCGCGCTGCGTGAAGATCCTGATGTGATCCTTGTGGGTGAGATGCGTGACCTTGAAACCATTAGACTGG
CGATGACGGCGGCAGAAACGGGTCACTTAGTCTTTGGTACCTTACATACCACCTCGGCGGCGAAAACCATTGACCGTGTA
GTCGACGTTTTCCCCGAAGGTGAAAAAAGCATGGTCAGAACCATGTTATCTGAATCACTGCAAGCGGTTATCTCGCAAAC
CCTTATCAAGAAAGTGGGCGGCGGCCGTGTTGCCGCACATGAGATCATGATGGGAACGCCTGCTATCCGTAACCTGATTC
GCGAAGATAAGGTGGCGCAGATGTACTCGGCAATTCAGACCGGTATGGCGCATGGAATGCAGACACTCGATCAATGTTTA
CAAAATTTGGTTAATCGAGGCCTTATCACTCGCGAAGATGCGATGTCTAAGAGTTCGAATAAGAATGCTAGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.838

96.812

0.783
  pilT Acinetobacter baumannii D1279779

80.838

96.812

0.783
  pilT Acinetobacter baumannii strain A118

80.838

96.812

0.783
  pilT Pseudomonas aeruginosa PAK

78.698

97.971

0.771
  pilT Pseudomonas stutzeri DSM 10701

78.698

97.971

0.771
  pilT Acinetobacter baylyi ADP1

79.331

95.362

0.757
  pilT Legionella pneumophila strain Lp02

74.561

99.13

0.739
  pilT Legionella pneumophila strain ERS1305867

74.561

99.13

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.42

0.412
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Acinetobacter baylyi ADP1

39.535

99.71

0.394