Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K0I62_RS14335 Genome accession   NZ_CP080419
Coordinates   3236913..3238025 (-) Length   370 a.a.
NCBI ID   WP_220068758.1    Uniprot ID   -
Organism   Shewanella psychrotolerans strain FJAT-53749     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3231913..3243025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0I62_RS14310 (K0I62_14310) lolB 3232173..3232829 (+) 657 WP_220068754.1 lipoprotein insertase outer membrane protein LolB -
  K0I62_RS14315 (K0I62_14315) ispE 3232819..3233673 (+) 855 WP_220068755.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  K0I62_RS14320 (K0I62_14320) - 3233741..3234688 (+) 948 WP_220063398.1 ribose-phosphate pyrophosphokinase -
  K0I62_RS14325 (K0I62_14325) hemH 3235204..3236220 (-) 1017 WP_220068756.1 ferrochelatase -
  K0I62_RS14330 (K0I62_14330) - 3236245..3236787 (-) 543 WP_220068757.1 glutathione peroxidase -
  K0I62_RS14335 (K0I62_14335) pilU 3236913..3238025 (-) 1113 WP_220068758.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0I62_RS14340 (K0I62_14340) pilT 3238039..3239076 (-) 1038 WP_220068759.1 type IV pilus twitching motility protein PilT Machinery gene
  K0I62_RS14345 (K0I62_14345) - 3239112..3239822 (+) 711 WP_220068760.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0I62_RS14350 (K0I62_14350) proC 3239895..3240713 (+) 819 WP_220068761.1 pyrroline-5-carboxylate reductase -
  K0I62_RS14355 (K0I62_14355) - 3240751..3241299 (+) 549 WP_220068762.1 YggT family protein -
  K0I62_RS14360 (K0I62_14360) yggU 3241299..3241586 (+) 288 WP_220068763.1 DUF167 family protein YggU -
  K0I62_RS14365 (K0I62_14365) - 3241679..3242113 (+) 435 WP_220068764.1 DUF4426 domain-containing protein -
  K0I62_RS14370 (K0I62_14370) rdgB 3242254..3242856 (+) 603 WP_220068765.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41282.33 Da        Isoelectric Point: 6.1389

>NTDB_id=592786 K0I62_RS14335 WP_220068758.1 3236913..3238025(-) (pilU) [Shewanella psychrotolerans strain FJAT-53749]
MEVRPFLKVMIDRKASDLFITAGFPPSAKIDGEVRPLGENPFTPEQSLEFVESLMTEAQRKEFHESRECNFAFAAKDLGR
FRVSAFWQRESPGCVMRRIETQIPEVEDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHNHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRIASLIQKNELHLLKETMAKSNEQGMQTFDQA
LLKLYSENEISYADALHYADSPNDLRLMIKLQSKEGASSGFMEGVTLDLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=592786 K0I62_RS14335 WP_220068758.1 3236913..3238025(-) (pilU) [Shewanella psychrotolerans strain FJAT-53749]
ATGGAAGTTCGTCCATTTTTAAAAGTAATGATAGATCGTAAAGCATCGGATCTGTTTATTACCGCTGGGTTTCCACCGAG
TGCCAAAATCGATGGCGAGGTGAGACCGTTAGGGGAGAATCCGTTTACCCCAGAGCAATCGTTAGAGTTTGTTGAATCGT
TAATGACAGAGGCTCAGCGTAAAGAGTTCCATGAAAGCCGTGAATGTAACTTTGCGTTTGCCGCTAAAGATCTGGGACGT
TTTCGTGTGAGTGCCTTTTGGCAGCGTGAGTCTCCTGGCTGCGTCATGCGTCGAATAGAAACTCAAATTCCGGAAGTCGA
AGATTTAAAGTTACCCCCTATACTAAAAGATCTGGTCATGAGTAAACGTGGCTTGATCATCATGGTGGGTGGTACGGGTA
CCGGTAAATCTACTTCGCTTGCGGCGCTAGTGGGTTATCGTAATGCCCATGCTCGTGGACATATTTTAACCATCGAAGAC
CCAGTCGAATTTGTTCACAATCATCGCAAGAGCATTATTACTCAGCGCGAAGTGGGTATTGATACCGAGTCGTTTGATGC
AGCGCTCAAGAGTTCGCTACGCCAAGCTCCAGATGTGATTTTGATTGGTGAGATCCGTAGTCAAGAAACTATGGAGTTCG
CGTTGTCATTTGCCGAGACTGGGCATTTGTGTATGGCAACTTTGCATGCCAACAATGCTAACCAAGCGTTAGATCGTATT
ATGCACTTGGTGCCTGAGAGTAAGCATCAACAGCTGTTATTCGATCTGTCGCTCAATCTGCGTGGTATTGTTGCGCAACA
ACTGGTACCCAAGAGTGATGGCAGCGGTCGACGAGCCGCCATTGAGGTATTGATCAATACGCCTCGTATTGCCAGTCTTA
TTCAGAAAAATGAGCTGCACCTGCTAAAAGAAACGATGGCTAAATCTAATGAGCAGGGGATGCAGACATTCGATCAGGCT
TTGCTTAAGCTTTATTCAGAGAATGAGATTAGTTATGCAGACGCACTGCACTATGCTGATTCTCCAAATGATCTACGCTT
AATGATTAAATTGCAGAGTAAAGAAGGCGCAAGCTCGGGCTTTATGGAAGGGGTGACCTTAGATCTCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Vibrio cholerae strain A1552

58.378

100

0.584
  pilU Acinetobacter baylyi ADP1

60.227

95.135

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.449

93.243

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

44.514

86.216

0.384
  pilT Vibrio cholerae strain A1552

44.514

86.216

0.384
  pilT Legionella pneumophila strain Lp02

41.692

89.459

0.373
  pilT Legionella pneumophila strain ERS1305867

41.692

89.459

0.373
  pilT Acinetobacter nosocomialis M2

39.474

92.432

0.365
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362