Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   K0H81_RS14405 Genome accession   NZ_CP080417
Coordinates   3318032..3319069 (-) Length   345 a.a.
NCBI ID   WP_011864942.1    Uniprot ID   A3QC11
Organism   Shewanella halotolerans strain FJAT-53555     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3313032..3324069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H81_RS14385 (K0H81_14385) - 3313404..3314606 (-) 1203 WP_220058389.1 IS4 family transposase -
  K0H81_RS14390 (K0H81_14390) - 3314650..3315057 (-) 408 WP_220058767.1 hypothetical protein -
  K0H81_RS14395 (K0H81_14395) hemH 3315713..3316726 (-) 1014 WP_220058768.1 ferrochelatase -
  K0H81_RS14400 (K0H81_14400) pilU 3316907..3318019 (-) 1113 WP_011864943.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K0H81_RS14405 (K0H81_14405) pilT 3318032..3319069 (-) 1038 WP_011864942.1 type IV pilus twitching motility protein PilT Machinery gene
  K0H81_RS14410 (K0H81_14410) - 3319105..3319806 (+) 702 WP_220058769.1 YggS family pyridoxal phosphate-dependent enzyme -
  K0H81_RS14415 (K0H81_14415) proC 3319891..3320709 (+) 819 WP_220058770.1 pyrroline-5-carboxylate reductase -
  K0H81_RS14420 (K0H81_14420) - 3320769..3321317 (+) 549 WP_144201230.1 YggT family protein -
  K0H81_RS14425 (K0H81_14425) yggU 3321318..3321608 (+) 291 WP_220044600.1 DUF167 family protein YggU -
  K0H81_RS14430 (K0H81_14430) - 3321695..3322129 (+) 435 WP_011864937.1 DUF4426 domain-containing protein -
  K0H81_RS14435 (K0H81_14435) rdgB 3322271..3322870 (+) 600 WP_220058771.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  K0H81_RS14440 (K0H81_14440) hemW 3322870..3324009 (+) 1140 WP_220058772.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38271.86 Da        Isoelectric Point: 6.9096

>NTDB_id=592744 K0H81_RS14405 WP_011864942.1 3318032..3319069(-) (pilT) [Shewanella halotolerans strain FJAT-53555]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEKLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAGMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKSMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGLITREDAMAKSSNKNASF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=592744 K0H81_RS14405 WP_011864942.1 3318032..3319069(-) (pilT) [Shewanella halotolerans strain FJAT-53555]
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAACGCGTCGGATCTACACCTTTCTGCGGGAGTCTCTCCCAT
GATACGTGTTGATGGTGAGGTCAGAAAGATTAATCTGCCGGCCCTGGATCATCAAGGCGTACACAGCCTCGTTTATGACA
TCATGAACGACAAACAGCGTAAAGATTATGAAGAGCATTTAGAGATAGATTTCTCGTTTGAAGTGCCCAACTTAGCCCGT
TTCCGTGTGAACGCCTTTAACCAGTCCCGCGGCGCCGCGGCGGTGTTCCGAACCATTCCCAGCGAGATCTTAAGCCTGGA
GAAACTGGGCGCCCCTGAGATTTTCAAGAAGATTGCCAGCTTTCCCCGTGGTCTGGTGTTGGTAACCGGCCCGACAGGTT
CGGGTAAGAGTACCACCCTGGCGGGCATGATAGATTATGTAAACGAGAACCGTCACGACCATATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCACCAGAACAAGCAGTGTCTGATCAACCAACGTGAGGTGCACAGACACACCCACAGCTTCAACGC
CGCGCTGCGTAGCGCACTGCGTGAAGACCCGGACGTGATCCTGGTGGGTGAGATGCGTGACCTCGAAACCATACGTCTGG
CGATGACGGCGGCGGAAACCGGTCACTTAGTATTTGGTACCCTGCACACCACCTCGGCGGCGAAAACCATCGACCGTGTG
GTCGACGTATTCCCCGAGGGCGAGAAGAGTATGGTGCGTACCATGTTGTCTGAGTCCCTGCAGGCGGTAATCTCCCAGAC
CCTGATCAAGAAGGTCGGCGGCGGTCGTGTGGCGGCCCACGAGATCATGATGGGTACCCCGGCGATTCGTAACCTTATTC
GCGAAGATAAGGTGGCACAGATGTACTCGGCCATTCAAACGGGTATGGCCCACGGCATGCAGACCCTAGATCAGTGTCTG
CAGAATCTGGTCAACCGTGGCCTGATCACCCGTGAAGATGCGATGGCCAAGAGTTCTAATAAAAACGCTAGTTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A3QC11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

81.138

96.812

0.786
  pilT Acinetobacter baumannii D1279779

81.138

96.812

0.786
  pilT Acinetobacter baumannii strain A118

81.138

96.812

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.402

97.971

0.768
  pilT Pseudomonas aeruginosa PAK

78.402

97.971

0.768
  pilT Acinetobacter baylyi ADP1

78.571

97.391

0.765
  pilT Legionella pneumophila strain Lp02

75.146

99.13

0.745
  pilT Legionella pneumophila strain ERS1305867

75.146

99.13

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.404

97.681

0.707
  pilT Vibrio cholerae strain A1552

72.404

97.681

0.707
  pilT Neisseria meningitidis 8013

69.186

99.71

0.69
  pilT Neisseria gonorrhoeae MS11

68.895

99.71

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.442

98.261

0.496
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

40.525

99.42

0.403
  pilU Acinetobacter baylyi ADP1

39.535

99.71

0.394